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Characterization of the Liriodendron Chinense MYB Gene Family and Its Role in Abiotic Stress Response

Liriodendron chinense (Lchi) is a Magnoliaceae plant, which is a basic angiosperm left behind by the Pleistocene and mainly distributed in the south of the Yangtze River. Liriodendron hybrids has good wood properties and is widely used in furniture and in other fields. It is not clear if they can ad...

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Autores principales: Wu, Weihuang, Zhu, Sheng, Zhu, Liming, Wang, Dandan, Liu, Yang, Liu, Siqin, Zhang, Jiaji, Hao, Zhaodong, Lu, Ye, Cheng, Tielong, Shi, Jisen, Chen, Jinhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350534/
https://www.ncbi.nlm.nih.gov/pubmed/34381467
http://dx.doi.org/10.3389/fpls.2021.641280
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author Wu, Weihuang
Zhu, Sheng
Zhu, Liming
Wang, Dandan
Liu, Yang
Liu, Siqin
Zhang, Jiaji
Hao, Zhaodong
Lu, Ye
Cheng, Tielong
Shi, Jisen
Chen, Jinhui
author_facet Wu, Weihuang
Zhu, Sheng
Zhu, Liming
Wang, Dandan
Liu, Yang
Liu, Siqin
Zhang, Jiaji
Hao, Zhaodong
Lu, Ye
Cheng, Tielong
Shi, Jisen
Chen, Jinhui
author_sort Wu, Weihuang
collection PubMed
description Liriodendron chinense (Lchi) is a Magnoliaceae plant, which is a basic angiosperm left behind by the Pleistocene and mainly distributed in the south of the Yangtze River. Liriodendron hybrids has good wood properties and is widely used in furniture and in other fields. It is not clear if they can adapt to different environmental conditions, such as drought and high and low temperatures, and the molecular mechanisms for this adaptation are unknown. Among plant transcription factors (TFs), the MYB gene family is one of the largest and is often involved in stress or adversity response signaling, growth, and development. Therefore, studying the role of MYBTFs in regulating abiotic stress signaling, growth, and development in Lchi is helpful to promote afforestation in different environments. In our research, a genome-wide analysis of the LchiMYB gene family was performed, including the phylogenetic relationship tree, gene exon-intron structure, collinearity, and chromosomal position. According to the evolutionary tree, 190 LchiMYBs were divided into three main branches. LchiMYBs were evenly distributed across 19 chromosomes, with their collinearity, suggesting that segment duplication events may have contributed to LchiMYB gene expansion. Transcriptomes from eight tissues, 11 stages of somatic embryogenesis, and leaves after cold, heat, and drought stress were used to analyze the function of the MYB gene family. The results of tissue expression analysis showed that most LchiMYB genes regulated bark, leaf, bud, sepal, stigma, and stamen development, as well as the four important stages (ES3, ES4, ES9, and PL) of somatic embryogenesis. More than 60 LchiMYBs responded to heat, cold, and drought stress; some of which underwent gene duplication during evolution. LchiMYB3 was highly expressed under all three forms of stress, while LchiMYB121 was strongly induced by both cold and heat stress. Eight genes with different expression patterns were selected and verified by quantitative real-time PCR (qRT-PCR) experiments. The results suggested that these LchiMYBs may regulate Lchi growth development and resistance to abiotic stress. This study shows the cross-regulatory function of LchiMYBs in the growth and development, asexual reproduction, and abiotic resistance of Lchi. This information will prove pivotal to directing further studies on the biological function of Lchi MYBTFs in genetic improvement and abiotic stress response.
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spelling pubmed-83505342021-08-10 Characterization of the Liriodendron Chinense MYB Gene Family and Its Role in Abiotic Stress Response Wu, Weihuang Zhu, Sheng Zhu, Liming Wang, Dandan Liu, Yang Liu, Siqin Zhang, Jiaji Hao, Zhaodong Lu, Ye Cheng, Tielong Shi, Jisen Chen, Jinhui Front Plant Sci Plant Science Liriodendron chinense (Lchi) is a Magnoliaceae plant, which is a basic angiosperm left behind by the Pleistocene and mainly distributed in the south of the Yangtze River. Liriodendron hybrids has good wood properties and is widely used in furniture and in other fields. It is not clear if they can adapt to different environmental conditions, such as drought and high and low temperatures, and the molecular mechanisms for this adaptation are unknown. Among plant transcription factors (TFs), the MYB gene family is one of the largest and is often involved in stress or adversity response signaling, growth, and development. Therefore, studying the role of MYBTFs in regulating abiotic stress signaling, growth, and development in Lchi is helpful to promote afforestation in different environments. In our research, a genome-wide analysis of the LchiMYB gene family was performed, including the phylogenetic relationship tree, gene exon-intron structure, collinearity, and chromosomal position. According to the evolutionary tree, 190 LchiMYBs were divided into three main branches. LchiMYBs were evenly distributed across 19 chromosomes, with their collinearity, suggesting that segment duplication events may have contributed to LchiMYB gene expansion. Transcriptomes from eight tissues, 11 stages of somatic embryogenesis, and leaves after cold, heat, and drought stress were used to analyze the function of the MYB gene family. The results of tissue expression analysis showed that most LchiMYB genes regulated bark, leaf, bud, sepal, stigma, and stamen development, as well as the four important stages (ES3, ES4, ES9, and PL) of somatic embryogenesis. More than 60 LchiMYBs responded to heat, cold, and drought stress; some of which underwent gene duplication during evolution. LchiMYB3 was highly expressed under all three forms of stress, while LchiMYB121 was strongly induced by both cold and heat stress. Eight genes with different expression patterns were selected and verified by quantitative real-time PCR (qRT-PCR) experiments. The results suggested that these LchiMYBs may regulate Lchi growth development and resistance to abiotic stress. This study shows the cross-regulatory function of LchiMYBs in the growth and development, asexual reproduction, and abiotic resistance of Lchi. This information will prove pivotal to directing further studies on the biological function of Lchi MYBTFs in genetic improvement and abiotic stress response. Frontiers Media S.A. 2021-07-26 /pmc/articles/PMC8350534/ /pubmed/34381467 http://dx.doi.org/10.3389/fpls.2021.641280 Text en Copyright © 2021 Wu, Zhu, Zhu, Wang, Liu, Liu, Zhang, Hao, Lu, Cheng, Shi and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wu, Weihuang
Zhu, Sheng
Zhu, Liming
Wang, Dandan
Liu, Yang
Liu, Siqin
Zhang, Jiaji
Hao, Zhaodong
Lu, Ye
Cheng, Tielong
Shi, Jisen
Chen, Jinhui
Characterization of the Liriodendron Chinense MYB Gene Family and Its Role in Abiotic Stress Response
title Characterization of the Liriodendron Chinense MYB Gene Family and Its Role in Abiotic Stress Response
title_full Characterization of the Liriodendron Chinense MYB Gene Family and Its Role in Abiotic Stress Response
title_fullStr Characterization of the Liriodendron Chinense MYB Gene Family and Its Role in Abiotic Stress Response
title_full_unstemmed Characterization of the Liriodendron Chinense MYB Gene Family and Its Role in Abiotic Stress Response
title_short Characterization of the Liriodendron Chinense MYB Gene Family and Its Role in Abiotic Stress Response
title_sort characterization of the liriodendron chinense myb gene family and its role in abiotic stress response
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8350534/
https://www.ncbi.nlm.nih.gov/pubmed/34381467
http://dx.doi.org/10.3389/fpls.2021.641280
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