Cargando…

Ovary Development: Insights From a Three-Dimensional Imaging Revolution

The ovary is an indispensable unit of female reproduction and health. However, the study of ovarian function in mammals is hindered by unique challenges, which include the desynchronized development of oocytes, irregular distribution and vast size discrepancy of follicles, and dynamic tissue remodel...

Descripción completa

Detalles Bibliográficos
Autores principales: Soygur, Bikem, Laird, Diana J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351467/
https://www.ncbi.nlm.nih.gov/pubmed/34381780
http://dx.doi.org/10.3389/fcell.2021.698315
_version_ 1783735981253328896
author Soygur, Bikem
Laird, Diana J.
author_facet Soygur, Bikem
Laird, Diana J.
author_sort Soygur, Bikem
collection PubMed
description The ovary is an indispensable unit of female reproduction and health. However, the study of ovarian function in mammals is hindered by unique challenges, which include the desynchronized development of oocytes, irregular distribution and vast size discrepancy of follicles, and dynamic tissue remodeling during each hormonal cycle. Overcoming the limitations of traditional histology, recent advances in optical tissue clearing and three-dimensional (3D) visualization offer an advanced platform to explore the architecture of intact organs at a single cell level and reveal new relationships and levels of organization. Here we summarize the development and function of ovarian compartments that have been delineated by conventional two-dimensional (2D) methods and the limits of what can be learned by these approaches. We compare types of optical tissue clearing, 3D analysis technologies, and their application to the mammalian ovary. We discuss how 3D modeling of the ovary has extended our knowledge and propose future directions to unravel ovarian structure toward therapeutic applications for ovarian disease and extending female reproductive lifespan.
format Online
Article
Text
id pubmed-8351467
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-83514672021-08-10 Ovary Development: Insights From a Three-Dimensional Imaging Revolution Soygur, Bikem Laird, Diana J. Front Cell Dev Biol Cell and Developmental Biology The ovary is an indispensable unit of female reproduction and health. However, the study of ovarian function in mammals is hindered by unique challenges, which include the desynchronized development of oocytes, irregular distribution and vast size discrepancy of follicles, and dynamic tissue remodeling during each hormonal cycle. Overcoming the limitations of traditional histology, recent advances in optical tissue clearing and three-dimensional (3D) visualization offer an advanced platform to explore the architecture of intact organs at a single cell level and reveal new relationships and levels of organization. Here we summarize the development and function of ovarian compartments that have been delineated by conventional two-dimensional (2D) methods and the limits of what can be learned by these approaches. We compare types of optical tissue clearing, 3D analysis technologies, and their application to the mammalian ovary. We discuss how 3D modeling of the ovary has extended our knowledge and propose future directions to unravel ovarian structure toward therapeutic applications for ovarian disease and extending female reproductive lifespan. Frontiers Media S.A. 2021-07-26 /pmc/articles/PMC8351467/ /pubmed/34381780 http://dx.doi.org/10.3389/fcell.2021.698315 Text en Copyright © 2021 Soygur and Laird. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Soygur, Bikem
Laird, Diana J.
Ovary Development: Insights From a Three-Dimensional Imaging Revolution
title Ovary Development: Insights From a Three-Dimensional Imaging Revolution
title_full Ovary Development: Insights From a Three-Dimensional Imaging Revolution
title_fullStr Ovary Development: Insights From a Three-Dimensional Imaging Revolution
title_full_unstemmed Ovary Development: Insights From a Three-Dimensional Imaging Revolution
title_short Ovary Development: Insights From a Three-Dimensional Imaging Revolution
title_sort ovary development: insights from a three-dimensional imaging revolution
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351467/
https://www.ncbi.nlm.nih.gov/pubmed/34381780
http://dx.doi.org/10.3389/fcell.2021.698315
work_keys_str_mv AT soygurbikem ovarydevelopmentinsightsfromathreedimensionalimagingrevolution
AT lairddianaj ovarydevelopmentinsightsfromathreedimensionalimagingrevolution