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Ovary Development: Insights From a Three-Dimensional Imaging Revolution
The ovary is an indispensable unit of female reproduction and health. However, the study of ovarian function in mammals is hindered by unique challenges, which include the desynchronized development of oocytes, irregular distribution and vast size discrepancy of follicles, and dynamic tissue remodel...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351467/ https://www.ncbi.nlm.nih.gov/pubmed/34381780 http://dx.doi.org/10.3389/fcell.2021.698315 |
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author | Soygur, Bikem Laird, Diana J. |
author_facet | Soygur, Bikem Laird, Diana J. |
author_sort | Soygur, Bikem |
collection | PubMed |
description | The ovary is an indispensable unit of female reproduction and health. However, the study of ovarian function in mammals is hindered by unique challenges, which include the desynchronized development of oocytes, irregular distribution and vast size discrepancy of follicles, and dynamic tissue remodeling during each hormonal cycle. Overcoming the limitations of traditional histology, recent advances in optical tissue clearing and three-dimensional (3D) visualization offer an advanced platform to explore the architecture of intact organs at a single cell level and reveal new relationships and levels of organization. Here we summarize the development and function of ovarian compartments that have been delineated by conventional two-dimensional (2D) methods and the limits of what can be learned by these approaches. We compare types of optical tissue clearing, 3D analysis technologies, and their application to the mammalian ovary. We discuss how 3D modeling of the ovary has extended our knowledge and propose future directions to unravel ovarian structure toward therapeutic applications for ovarian disease and extending female reproductive lifespan. |
format | Online Article Text |
id | pubmed-8351467 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83514672021-08-10 Ovary Development: Insights From a Three-Dimensional Imaging Revolution Soygur, Bikem Laird, Diana J. Front Cell Dev Biol Cell and Developmental Biology The ovary is an indispensable unit of female reproduction and health. However, the study of ovarian function in mammals is hindered by unique challenges, which include the desynchronized development of oocytes, irregular distribution and vast size discrepancy of follicles, and dynamic tissue remodeling during each hormonal cycle. Overcoming the limitations of traditional histology, recent advances in optical tissue clearing and three-dimensional (3D) visualization offer an advanced platform to explore the architecture of intact organs at a single cell level and reveal new relationships and levels of organization. Here we summarize the development and function of ovarian compartments that have been delineated by conventional two-dimensional (2D) methods and the limits of what can be learned by these approaches. We compare types of optical tissue clearing, 3D analysis technologies, and their application to the mammalian ovary. We discuss how 3D modeling of the ovary has extended our knowledge and propose future directions to unravel ovarian structure toward therapeutic applications for ovarian disease and extending female reproductive lifespan. Frontiers Media S.A. 2021-07-26 /pmc/articles/PMC8351467/ /pubmed/34381780 http://dx.doi.org/10.3389/fcell.2021.698315 Text en Copyright © 2021 Soygur and Laird. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Soygur, Bikem Laird, Diana J. Ovary Development: Insights From a Three-Dimensional Imaging Revolution |
title | Ovary Development: Insights From a Three-Dimensional Imaging Revolution |
title_full | Ovary Development: Insights From a Three-Dimensional Imaging Revolution |
title_fullStr | Ovary Development: Insights From a Three-Dimensional Imaging Revolution |
title_full_unstemmed | Ovary Development: Insights From a Three-Dimensional Imaging Revolution |
title_short | Ovary Development: Insights From a Three-Dimensional Imaging Revolution |
title_sort | ovary development: insights from a three-dimensional imaging revolution |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351467/ https://www.ncbi.nlm.nih.gov/pubmed/34381780 http://dx.doi.org/10.3389/fcell.2021.698315 |
work_keys_str_mv | AT soygurbikem ovarydevelopmentinsightsfromathreedimensionalimagingrevolution AT lairddianaj ovarydevelopmentinsightsfromathreedimensionalimagingrevolution |