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Epigenetic signatures differentiate uterine and soft tissue leiomyosarcoma
Leiomyosarcomas (LMS) are diverse, rare, and aggressive mesenchymal soft tissue sarcomas. Epigenetic alterations influence multiple aspects of cancer, however epigenetic profiling of LMS has been limited. The goal of this study was to delineate the molecular landscape of LMS for subtype-specific dif...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351604/ https://www.ncbi.nlm.nih.gov/pubmed/34381562 http://dx.doi.org/10.18632/oncotarget.28032 |
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author | Hasan, Nesrin M. Sharma, Anup Ruzgar, Nensi M. Deshpande, Hari Olino, Kelly Khan, Sajid Ahuja, Nita |
author_facet | Hasan, Nesrin M. Sharma, Anup Ruzgar, Nensi M. Deshpande, Hari Olino, Kelly Khan, Sajid Ahuja, Nita |
author_sort | Hasan, Nesrin M. |
collection | PubMed |
description | Leiomyosarcomas (LMS) are diverse, rare, and aggressive mesenchymal soft tissue sarcomas. Epigenetic alterations influence multiple aspects of cancer, however epigenetic profiling of LMS has been limited. The goal of this study was to delineate the molecular landscape of LMS for subtype-specific differences (uterine LMS (ULMS) vs soft tissue LMS (STLMS)) based on integrated analysis of DNA methylation and gene expression to identify potential targets for therapeutic intervention and diagnosis. We identified differentially methylated and differentially expressed genes associated with ULMS and STLMS using DNA methylation and RNA-seq data from primary tumors. Two main clusters were identified through unsupervised hierarchical clustering: ULMS-enriched cluster and STLMS-enriched cluster. The integrated analysis demonstrated 34 genes associated with hypermethylation of the promoter CpG islands and downregulation of gene expression in ULMS or STLMS. In summary, these results indicate that differential DNA methylation and gene expression patterns are associated with ULMS and STLMS. Further studies are needed to delineate the contribution of epigenetic regulation to LMS subtype-specific gene expression and determine the roles of the differentially methylated and differentially expressed genes as potential therapeutic targets or biomarkers. |
format | Online Article Text |
id | pubmed-8351604 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-83516042021-08-10 Epigenetic signatures differentiate uterine and soft tissue leiomyosarcoma Hasan, Nesrin M. Sharma, Anup Ruzgar, Nensi M. Deshpande, Hari Olino, Kelly Khan, Sajid Ahuja, Nita Oncotarget Research Paper Leiomyosarcomas (LMS) are diverse, rare, and aggressive mesenchymal soft tissue sarcomas. Epigenetic alterations influence multiple aspects of cancer, however epigenetic profiling of LMS has been limited. The goal of this study was to delineate the molecular landscape of LMS for subtype-specific differences (uterine LMS (ULMS) vs soft tissue LMS (STLMS)) based on integrated analysis of DNA methylation and gene expression to identify potential targets for therapeutic intervention and diagnosis. We identified differentially methylated and differentially expressed genes associated with ULMS and STLMS using DNA methylation and RNA-seq data from primary tumors. Two main clusters were identified through unsupervised hierarchical clustering: ULMS-enriched cluster and STLMS-enriched cluster. The integrated analysis demonstrated 34 genes associated with hypermethylation of the promoter CpG islands and downregulation of gene expression in ULMS or STLMS. In summary, these results indicate that differential DNA methylation and gene expression patterns are associated with ULMS and STLMS. Further studies are needed to delineate the contribution of epigenetic regulation to LMS subtype-specific gene expression and determine the roles of the differentially methylated and differentially expressed genes as potential therapeutic targets or biomarkers. Impact Journals LLC 2021-08-03 /pmc/articles/PMC8351604/ /pubmed/34381562 http://dx.doi.org/10.18632/oncotarget.28032 Text en Copyright: © 2021 Hasan et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Hasan, Nesrin M. Sharma, Anup Ruzgar, Nensi M. Deshpande, Hari Olino, Kelly Khan, Sajid Ahuja, Nita Epigenetic signatures differentiate uterine and soft tissue leiomyosarcoma |
title | Epigenetic signatures differentiate uterine and soft tissue leiomyosarcoma |
title_full | Epigenetic signatures differentiate uterine and soft tissue leiomyosarcoma |
title_fullStr | Epigenetic signatures differentiate uterine and soft tissue leiomyosarcoma |
title_full_unstemmed | Epigenetic signatures differentiate uterine and soft tissue leiomyosarcoma |
title_short | Epigenetic signatures differentiate uterine and soft tissue leiomyosarcoma |
title_sort | epigenetic signatures differentiate uterine and soft tissue leiomyosarcoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351604/ https://www.ncbi.nlm.nih.gov/pubmed/34381562 http://dx.doi.org/10.18632/oncotarget.28032 |
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