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Cross-talk-dependent cortical patterning of Rho GTPases during cell repair

Rho GTPases such as Rho, Rac, and Cdc42 are important regulators of the cortical cytoskeleton in processes including cell division, locomotion, and repair. In these processes, Rho GTPases assume characteristic patterns wherein the active GTPases occupy mutually exclusive “zones” in the cell cortex....

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Autores principales: Moe, Alison, Holmes, William, Golding, Adriana E., Zola, Jessica, Swider, Zachary T., Edelstein-Keshet, Leah, Bement, William
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351735/
https://www.ncbi.nlm.nih.gov/pubmed/34133216
http://dx.doi.org/10.1091/mbc.E20-07-0481
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author Moe, Alison
Holmes, William
Golding, Adriana E.
Zola, Jessica
Swider, Zachary T.
Edelstein-Keshet, Leah
Bement, William
author_facet Moe, Alison
Holmes, William
Golding, Adriana E.
Zola, Jessica
Swider, Zachary T.
Edelstein-Keshet, Leah
Bement, William
author_sort Moe, Alison
collection PubMed
description Rho GTPases such as Rho, Rac, and Cdc42 are important regulators of the cortical cytoskeleton in processes including cell division, locomotion, and repair. In these processes, Rho GTPases assume characteristic patterns wherein the active GTPases occupy mutually exclusive “zones” in the cell cortex. During cell wound repair, for example, a Rho zone encircles the wound edge and is in turn encircled by a Cdc42 zone. Here we evaluated the contributions of cross-talk between Rho and Cdc42 to the patterning of their respective zones in wounded Xenopus oocytes using experimental manipulations in combination with mathematical modeling. The results show that the position of the Cdc42 zone relative to the Rho zone and relative to the wound edge is controlled by the level of Rho activity. In contrast, the outer boundary of the Rho zone is limited by the level of Cdc42 activity. Models based on positive feedback within zones and negative feedback from Rho to the GEF-GAP Abr to Cdc42 capture some, but not all, of the observed behaviors. We conclude that GTPase zone positioning is controlled at the level of Rho activity and we speculate that the Cdc42 zone or something associated with it limits the spread of Rho activity.
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spelling pubmed-83517352021-10-16 Cross-talk-dependent cortical patterning of Rho GTPases during cell repair Moe, Alison Holmes, William Golding, Adriana E. Zola, Jessica Swider, Zachary T. Edelstein-Keshet, Leah Bement, William Mol Biol Cell Articles Rho GTPases such as Rho, Rac, and Cdc42 are important regulators of the cortical cytoskeleton in processes including cell division, locomotion, and repair. In these processes, Rho GTPases assume characteristic patterns wherein the active GTPases occupy mutually exclusive “zones” in the cell cortex. During cell wound repair, for example, a Rho zone encircles the wound edge and is in turn encircled by a Cdc42 zone. Here we evaluated the contributions of cross-talk between Rho and Cdc42 to the patterning of their respective zones in wounded Xenopus oocytes using experimental manipulations in combination with mathematical modeling. The results show that the position of the Cdc42 zone relative to the Rho zone and relative to the wound edge is controlled by the level of Rho activity. In contrast, the outer boundary of the Rho zone is limited by the level of Cdc42 activity. Models based on positive feedback within zones and negative feedback from Rho to the GEF-GAP Abr to Cdc42 capture some, but not all, of the observed behaviors. We conclude that GTPase zone positioning is controlled at the level of Rho activity and we speculate that the Cdc42 zone or something associated with it limits the spread of Rho activity. The American Society for Cell Biology 2021-08-01 /pmc/articles/PMC8351735/ /pubmed/34133216 http://dx.doi.org/10.1091/mbc.E20-07-0481 Text en © 2021 Moe et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. https://creativecommons.org/licenses/by-nc-sa/3.0/This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Moe, Alison
Holmes, William
Golding, Adriana E.
Zola, Jessica
Swider, Zachary T.
Edelstein-Keshet, Leah
Bement, William
Cross-talk-dependent cortical patterning of Rho GTPases during cell repair
title Cross-talk-dependent cortical patterning of Rho GTPases during cell repair
title_full Cross-talk-dependent cortical patterning of Rho GTPases during cell repair
title_fullStr Cross-talk-dependent cortical patterning of Rho GTPases during cell repair
title_full_unstemmed Cross-talk-dependent cortical patterning of Rho GTPases during cell repair
title_short Cross-talk-dependent cortical patterning of Rho GTPases during cell repair
title_sort cross-talk-dependent cortical patterning of rho gtpases during cell repair
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351735/
https://www.ncbi.nlm.nih.gov/pubmed/34133216
http://dx.doi.org/10.1091/mbc.E20-07-0481
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