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CARD11 is a prognostic biomarker and correlated with immune infiltrates in uveal melanoma
Uveal melanoma (UVM), the most common primary intraocular malignancy, has a high mortality because of a high propensity to metastasize. Our study analyzed prognostic value and immune-related characteristics of CARD11 in UVM, hoping to provide a potential management and research direction. The RNA-se...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351993/ https://www.ncbi.nlm.nih.gov/pubmed/34370778 http://dx.doi.org/10.1371/journal.pone.0255293 |
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author | Shi, Xueying Xia, Shilin Chu, Yingming Yang, Nan Zheng, Jingyuan Chen, Qianyi Fen, Zeng Jiang, Yuankuan Fang, Shifeng Lin, Jingrong |
author_facet | Shi, Xueying Xia, Shilin Chu, Yingming Yang, Nan Zheng, Jingyuan Chen, Qianyi Fen, Zeng Jiang, Yuankuan Fang, Shifeng Lin, Jingrong |
author_sort | Shi, Xueying |
collection | PubMed |
description | Uveal melanoma (UVM), the most common primary intraocular malignancy, has a high mortality because of a high propensity to metastasize. Our study analyzed prognostic value and immune-related characteristics of CARD11 in UVM, hoping to provide a potential management and research direction. The RNA-sequence data of 80 UVM patients were downloaded from The Cancer Genome Atlas database and divided them into high- and low-expression groups. We analyzed the differentially expressed genes, enrichment analyses and the infiltration of immune cells using the R package and Gene-Set Enrichment Analysis. A clinical prediction nomogram and protein-protein interaction network were constructed and the first 8 genes were considered as the hub-genes. Finally, we constructed a competing endogenous RNA (ceRNA) network by Cytoscape and analyzed the statistical data via the R software. Here we found that CARD11 expression had notable correlation with UVM clinicopathological features, which was also an independent predictor for overall survival (OS). Intriguingly, CARD11 had a positively correlation to autophagy, cellular senescence and apoptosis. Infiltration of monocytes was significantly higher in low CARD11 expression group, and infiltration of T cells regulatory was lower in the same group. Functional enrichment analyses revealed that CARD11 was positively related to T cell activation pathways and cell adhesion molecules. The expressions of hub-genes were all increased in the high CARD11 expression group and the ceRNA network showed the interaction among mRNA, miRNA and lncRNA. These findings show that high CARD11 expression in UVM is associated with poor OS, indicating that CARD11 may serve as a potential biomarker for the diagnosis and prognosis of the UVM. |
format | Online Article Text |
id | pubmed-8351993 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-83519932021-08-10 CARD11 is a prognostic biomarker and correlated with immune infiltrates in uveal melanoma Shi, Xueying Xia, Shilin Chu, Yingming Yang, Nan Zheng, Jingyuan Chen, Qianyi Fen, Zeng Jiang, Yuankuan Fang, Shifeng Lin, Jingrong PLoS One Research Article Uveal melanoma (UVM), the most common primary intraocular malignancy, has a high mortality because of a high propensity to metastasize. Our study analyzed prognostic value and immune-related characteristics of CARD11 in UVM, hoping to provide a potential management and research direction. The RNA-sequence data of 80 UVM patients were downloaded from The Cancer Genome Atlas database and divided them into high- and low-expression groups. We analyzed the differentially expressed genes, enrichment analyses and the infiltration of immune cells using the R package and Gene-Set Enrichment Analysis. A clinical prediction nomogram and protein-protein interaction network were constructed and the first 8 genes were considered as the hub-genes. Finally, we constructed a competing endogenous RNA (ceRNA) network by Cytoscape and analyzed the statistical data via the R software. Here we found that CARD11 expression had notable correlation with UVM clinicopathological features, which was also an independent predictor for overall survival (OS). Intriguingly, CARD11 had a positively correlation to autophagy, cellular senescence and apoptosis. Infiltration of monocytes was significantly higher in low CARD11 expression group, and infiltration of T cells regulatory was lower in the same group. Functional enrichment analyses revealed that CARD11 was positively related to T cell activation pathways and cell adhesion molecules. The expressions of hub-genes were all increased in the high CARD11 expression group and the ceRNA network showed the interaction among mRNA, miRNA and lncRNA. These findings show that high CARD11 expression in UVM is associated with poor OS, indicating that CARD11 may serve as a potential biomarker for the diagnosis and prognosis of the UVM. Public Library of Science 2021-08-09 /pmc/articles/PMC8351993/ /pubmed/34370778 http://dx.doi.org/10.1371/journal.pone.0255293 Text en © 2021 Shi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Shi, Xueying Xia, Shilin Chu, Yingming Yang, Nan Zheng, Jingyuan Chen, Qianyi Fen, Zeng Jiang, Yuankuan Fang, Shifeng Lin, Jingrong CARD11 is a prognostic biomarker and correlated with immune infiltrates in uveal melanoma |
title | CARD11 is a prognostic biomarker and correlated with immune infiltrates in uveal melanoma |
title_full | CARD11 is a prognostic biomarker and correlated with immune infiltrates in uveal melanoma |
title_fullStr | CARD11 is a prognostic biomarker and correlated with immune infiltrates in uveal melanoma |
title_full_unstemmed | CARD11 is a prognostic biomarker and correlated with immune infiltrates in uveal melanoma |
title_short | CARD11 is a prognostic biomarker and correlated with immune infiltrates in uveal melanoma |
title_sort | card11 is a prognostic biomarker and correlated with immune infiltrates in uveal melanoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351993/ https://www.ncbi.nlm.nih.gov/pubmed/34370778 http://dx.doi.org/10.1371/journal.pone.0255293 |
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