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Purkinje cell outputs selectively inhibit a subset of unipolar brush cells in the input layer of the cerebellar cortex

Circuitry of the cerebellar cortex is regionally and functionally specialized. Unipolar brush cells (UBCs), and Purkinje cell (PC) synapses made by axon collaterals in the granular layer, are both enriched in areas that control balance and eye movement. Here, we find a link between these specializat...

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Autores principales: Guo, Chong, Rudolph, Stephanie, Neuwirth, Morgan E, Regehr, Wade G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352585/
https://www.ncbi.nlm.nih.gov/pubmed/34369877
http://dx.doi.org/10.7554/eLife.68802
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author Guo, Chong
Rudolph, Stephanie
Neuwirth, Morgan E
Regehr, Wade G
author_facet Guo, Chong
Rudolph, Stephanie
Neuwirth, Morgan E
Regehr, Wade G
author_sort Guo, Chong
collection PubMed
description Circuitry of the cerebellar cortex is regionally and functionally specialized. Unipolar brush cells (UBCs), and Purkinje cell (PC) synapses made by axon collaterals in the granular layer, are both enriched in areas that control balance and eye movement. Here, we find a link between these specializations in mice: PCs preferentially inhibit metabotropic glutamate receptor type 1 (mGluR1)-expressing UBCs that respond to mossy fiber (MF) inputs with long lasting increases in firing, but PCs do not inhibit mGluR1-lacking UBCs. PCs inhibit about 29% of mGluR1-expressing UBCs by activating GABA(A) receptors (GABA(A)Rs) and inhibit almost all mGluR1-expressing UBCs by activating GABA(B) receptors (GABA(B)Rs). PC to UBC synapses allow PC output to regulate the input layer of the cerebellar cortex in diverse ways. Based on optogenetic studies and a small number of paired recordings, GABA(A)R-mediated feedback is fast and unreliable. GABA(B)R-mediated inhibition is slower and is sufficiently large to strongly influence the input-output transformations of mGluR1-expressing UBCs.
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spelling pubmed-83525852021-08-11 Purkinje cell outputs selectively inhibit a subset of unipolar brush cells in the input layer of the cerebellar cortex Guo, Chong Rudolph, Stephanie Neuwirth, Morgan E Regehr, Wade G eLife Neuroscience Circuitry of the cerebellar cortex is regionally and functionally specialized. Unipolar brush cells (UBCs), and Purkinje cell (PC) synapses made by axon collaterals in the granular layer, are both enriched in areas that control balance and eye movement. Here, we find a link between these specializations in mice: PCs preferentially inhibit metabotropic glutamate receptor type 1 (mGluR1)-expressing UBCs that respond to mossy fiber (MF) inputs with long lasting increases in firing, but PCs do not inhibit mGluR1-lacking UBCs. PCs inhibit about 29% of mGluR1-expressing UBCs by activating GABA(A) receptors (GABA(A)Rs) and inhibit almost all mGluR1-expressing UBCs by activating GABA(B) receptors (GABA(B)Rs). PC to UBC synapses allow PC output to regulate the input layer of the cerebellar cortex in diverse ways. Based on optogenetic studies and a small number of paired recordings, GABA(A)R-mediated feedback is fast and unreliable. GABA(B)R-mediated inhibition is slower and is sufficiently large to strongly influence the input-output transformations of mGluR1-expressing UBCs. eLife Sciences Publications, Ltd 2021-08-09 /pmc/articles/PMC8352585/ /pubmed/34369877 http://dx.doi.org/10.7554/eLife.68802 Text en © 2021, Guo et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Guo, Chong
Rudolph, Stephanie
Neuwirth, Morgan E
Regehr, Wade G
Purkinje cell outputs selectively inhibit a subset of unipolar brush cells in the input layer of the cerebellar cortex
title Purkinje cell outputs selectively inhibit a subset of unipolar brush cells in the input layer of the cerebellar cortex
title_full Purkinje cell outputs selectively inhibit a subset of unipolar brush cells in the input layer of the cerebellar cortex
title_fullStr Purkinje cell outputs selectively inhibit a subset of unipolar brush cells in the input layer of the cerebellar cortex
title_full_unstemmed Purkinje cell outputs selectively inhibit a subset of unipolar brush cells in the input layer of the cerebellar cortex
title_short Purkinje cell outputs selectively inhibit a subset of unipolar brush cells in the input layer of the cerebellar cortex
title_sort purkinje cell outputs selectively inhibit a subset of unipolar brush cells in the input layer of the cerebellar cortex
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352585/
https://www.ncbi.nlm.nih.gov/pubmed/34369877
http://dx.doi.org/10.7554/eLife.68802
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