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Molecular characterization of projection neuron subtypes in the mouse olfactory bulb
Projection neurons (PNs) in the mammalian olfactory bulb (OB) receive input from the nose and project to diverse cortical and subcortical areas. Morphological and physiological studies have highlighted functional heterogeneity, yet no molecular markers have been described that delineate PN subtypes....
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352594/ https://www.ncbi.nlm.nih.gov/pubmed/34292150 http://dx.doi.org/10.7554/eLife.65445 |
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author | Zeppilli, Sara Ackels, Tobias Attey, Robin Klimpert, Nell Ritola, Kimberly D Boeing, Stefan Crombach, Anton Schaefer, Andreas T Fleischmann, Alexander |
author_facet | Zeppilli, Sara Ackels, Tobias Attey, Robin Klimpert, Nell Ritola, Kimberly D Boeing, Stefan Crombach, Anton Schaefer, Andreas T Fleischmann, Alexander |
author_sort | Zeppilli, Sara |
collection | PubMed |
description | Projection neurons (PNs) in the mammalian olfactory bulb (OB) receive input from the nose and project to diverse cortical and subcortical areas. Morphological and physiological studies have highlighted functional heterogeneity, yet no molecular markers have been described that delineate PN subtypes. Here, we used viral injections into olfactory cortex and fluorescent nucleus sorting to enrich PNs for high-throughput single nucleus and bulk RNA deep sequencing. Transcriptome analysis and RNA in situ hybridization identified distinct mitral and tufted cell populations with characteristic transcription factor network topology, cell adhesion, and excitability-related gene expression. Finally, we describe a new computational approach for integrating bulk and snRNA-seq data and provide evidence that different mitral cell populations preferentially project to different target regions. Together, we have identified potential molecular and gene regulatory mechanisms underlying PN diversity and provide new molecular entry points into studying the diverse functional roles of mitral and tufted cell subtypes. |
format | Online Article Text |
id | pubmed-8352594 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-83525942021-08-11 Molecular characterization of projection neuron subtypes in the mouse olfactory bulb Zeppilli, Sara Ackels, Tobias Attey, Robin Klimpert, Nell Ritola, Kimberly D Boeing, Stefan Crombach, Anton Schaefer, Andreas T Fleischmann, Alexander eLife Neuroscience Projection neurons (PNs) in the mammalian olfactory bulb (OB) receive input from the nose and project to diverse cortical and subcortical areas. Morphological and physiological studies have highlighted functional heterogeneity, yet no molecular markers have been described that delineate PN subtypes. Here, we used viral injections into olfactory cortex and fluorescent nucleus sorting to enrich PNs for high-throughput single nucleus and bulk RNA deep sequencing. Transcriptome analysis and RNA in situ hybridization identified distinct mitral and tufted cell populations with characteristic transcription factor network topology, cell adhesion, and excitability-related gene expression. Finally, we describe a new computational approach for integrating bulk and snRNA-seq data and provide evidence that different mitral cell populations preferentially project to different target regions. Together, we have identified potential molecular and gene regulatory mechanisms underlying PN diversity and provide new molecular entry points into studying the diverse functional roles of mitral and tufted cell subtypes. eLife Sciences Publications, Ltd 2021-07-22 /pmc/articles/PMC8352594/ /pubmed/34292150 http://dx.doi.org/10.7554/eLife.65445 Text en © 2021, Zeppilli et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Zeppilli, Sara Ackels, Tobias Attey, Robin Klimpert, Nell Ritola, Kimberly D Boeing, Stefan Crombach, Anton Schaefer, Andreas T Fleischmann, Alexander Molecular characterization of projection neuron subtypes in the mouse olfactory bulb |
title | Molecular characterization of projection neuron subtypes in the mouse olfactory bulb |
title_full | Molecular characterization of projection neuron subtypes in the mouse olfactory bulb |
title_fullStr | Molecular characterization of projection neuron subtypes in the mouse olfactory bulb |
title_full_unstemmed | Molecular characterization of projection neuron subtypes in the mouse olfactory bulb |
title_short | Molecular characterization of projection neuron subtypes in the mouse olfactory bulb |
title_sort | molecular characterization of projection neuron subtypes in the mouse olfactory bulb |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352594/ https://www.ncbi.nlm.nih.gov/pubmed/34292150 http://dx.doi.org/10.7554/eLife.65445 |
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