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“The effectiveness of rapid antigen test-based for SARS-CoV-2 detection in nursing homes in Valencia, Spain”
The usefulness of the PANBIO ™ COVID-19 Ag rapid test for SARS-CoV-2 infection detection has not been widely studied, especially in specific population groups such as the elderly who are institutionalized. Rapid diagnostic tests have the potential to benefit testing strategies, as they have short tu...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352650/ https://www.ncbi.nlm.nih.gov/pubmed/34399104 http://dx.doi.org/10.1016/j.jcv.2021.104941 |
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author | Escrivá, Begoña Fuster Mochón, María Dolores Ocete González, Rafael Medina García, Carme Salvador Pla, Ana Tornero Ricart, Amparo Sahuquillo García, Mercedes Melero Aranda, Irene Tur García, Fernando Grossón Cardona, Concepción Gimeno |
author_facet | Escrivá, Begoña Fuster Mochón, María Dolores Ocete González, Rafael Medina García, Carme Salvador Pla, Ana Tornero Ricart, Amparo Sahuquillo García, Mercedes Melero Aranda, Irene Tur García, Fernando Grossón Cardona, Concepción Gimeno |
author_sort | Escrivá, Begoña Fuster |
collection | PubMed |
description | The usefulness of the PANBIO ™ COVID-19 Ag rapid test for SARS-CoV-2 infection detection has not been widely studied, especially in specific population groups such as the elderly who are institutionalized. Rapid diagnostic tests have the potential to benefit testing strategies, as they have short turnaround times, they are cheap, simple to perform and can be used in decentralized testing. The objective of this study is to show the performance of the PANBIO™ COVID-19 Ag Rapid test device conducted at geriatric institutions and to compare results to those obtained from RT-PCR. A total of 448 individuals were enrolled in the study, including both residents and employees. Nasopharyngeal swabs were collected for both PANBIO™ COVID-19 Ag Rapid test and RT-PCR testing. All the samples were analyzed by specialized microbiologists. A total of 117 out of 448 individuals (26%) tested positive by RT-PCR, of whom 99 (85%) returned positive Antigen test results. There were 18 Antigen negative cases with positive RT-PCR results. Accordingly, concordance between RT-PCR and Antigen test results was acceptable (K index, 0.89; 95% IC 0.8455–0.9345). Overall sensitivity and specificity of Antigen test was 85% and 100%, respectively. When defining RT-PCR CT positivity on a cut-off value of 35, LFA sensitivity was 90%. In case a cut-off value of 30 was used, LFA would increase up to 99%. In this real-life evaluation of the PANBIO™ COVID-Ag rapid test, the assay reliably identified SARS-CoV-2 infected individuals with low CT-values by RT-PCR. False negative results were observed only at high CT-values, meaning low viral loads in nasopharyngeal samples. |
format | Online Article Text |
id | pubmed-8352650 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83526502021-08-10 “The effectiveness of rapid antigen test-based for SARS-CoV-2 detection in nursing homes in Valencia, Spain” Escrivá, Begoña Fuster Mochón, María Dolores Ocete González, Rafael Medina García, Carme Salvador Pla, Ana Tornero Ricart, Amparo Sahuquillo García, Mercedes Melero Aranda, Irene Tur García, Fernando Grossón Cardona, Concepción Gimeno J Clin Virol Article The usefulness of the PANBIO ™ COVID-19 Ag rapid test for SARS-CoV-2 infection detection has not been widely studied, especially in specific population groups such as the elderly who are institutionalized. Rapid diagnostic tests have the potential to benefit testing strategies, as they have short turnaround times, they are cheap, simple to perform and can be used in decentralized testing. The objective of this study is to show the performance of the PANBIO™ COVID-19 Ag Rapid test device conducted at geriatric institutions and to compare results to those obtained from RT-PCR. A total of 448 individuals were enrolled in the study, including both residents and employees. Nasopharyngeal swabs were collected for both PANBIO™ COVID-19 Ag Rapid test and RT-PCR testing. All the samples were analyzed by specialized microbiologists. A total of 117 out of 448 individuals (26%) tested positive by RT-PCR, of whom 99 (85%) returned positive Antigen test results. There were 18 Antigen negative cases with positive RT-PCR results. Accordingly, concordance between RT-PCR and Antigen test results was acceptable (K index, 0.89; 95% IC 0.8455–0.9345). Overall sensitivity and specificity of Antigen test was 85% and 100%, respectively. When defining RT-PCR CT positivity on a cut-off value of 35, LFA sensitivity was 90%. In case a cut-off value of 30 was used, LFA would increase up to 99%. In this real-life evaluation of the PANBIO™ COVID-Ag rapid test, the assay reliably identified SARS-CoV-2 infected individuals with low CT-values by RT-PCR. False negative results were observed only at high CT-values, meaning low viral loads in nasopharyngeal samples. Elsevier B.V. 2021-10 2021-08-10 /pmc/articles/PMC8352650/ /pubmed/34399104 http://dx.doi.org/10.1016/j.jcv.2021.104941 Text en © 2021 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Escrivá, Begoña Fuster Mochón, María Dolores Ocete González, Rafael Medina García, Carme Salvador Pla, Ana Tornero Ricart, Amparo Sahuquillo García, Mercedes Melero Aranda, Irene Tur García, Fernando Grossón Cardona, Concepción Gimeno “The effectiveness of rapid antigen test-based for SARS-CoV-2 detection in nursing homes in Valencia, Spain” |
title | “The effectiveness of rapid antigen test-based for SARS-CoV-2 detection in nursing homes in Valencia, Spain” |
title_full | “The effectiveness of rapid antigen test-based for SARS-CoV-2 detection in nursing homes in Valencia, Spain” |
title_fullStr | “The effectiveness of rapid antigen test-based for SARS-CoV-2 detection in nursing homes in Valencia, Spain” |
title_full_unstemmed | “The effectiveness of rapid antigen test-based for SARS-CoV-2 detection in nursing homes in Valencia, Spain” |
title_short | “The effectiveness of rapid antigen test-based for SARS-CoV-2 detection in nursing homes in Valencia, Spain” |
title_sort | “the effectiveness of rapid antigen test-based for sars-cov-2 detection in nursing homes in valencia, spain” |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352650/ https://www.ncbi.nlm.nih.gov/pubmed/34399104 http://dx.doi.org/10.1016/j.jcv.2021.104941 |
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