Cargando…

Long Noncoding RNA MIR100HG Knockdown Attenuates Hepatocellular Carcinoma Progression by Regulating MicroRNA-146b-5p/Chromobox 6

PURPOSE: Hepatocellular carcinoma (HCC) accounts for approximately ninety percent of primary liver cancer. This study attempted to investigate the effects of the long noncoding RNA MIR100HG (MIR100HG) in HCC and the underlying molecular mechanism. MATERIALS AND METHODS: qRT-PCR was implemented to an...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Fushun, Sun, Xianghua, Liu, Qing, Liu, Xilu, Zhang, Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352691/
https://www.ncbi.nlm.nih.gov/pubmed/34381502
http://dx.doi.org/10.1155/2021/6832518
_version_ 1783736238450147328
author Li, Fushun
Sun, Xianghua
Liu, Qing
Liu, Xilu
Zhang, Jia
author_facet Li, Fushun
Sun, Xianghua
Liu, Qing
Liu, Xilu
Zhang, Jia
author_sort Li, Fushun
collection PubMed
description PURPOSE: Hepatocellular carcinoma (HCC) accounts for approximately ninety percent of primary liver cancer. This study attempted to investigate the effects of the long noncoding RNA MIR100HG (MIR100HG) in HCC and the underlying molecular mechanism. MATERIALS AND METHODS: qRT-PCR was implemented to analyze the expression of MIR100HG, microRNA-146b-5p (miR-146b-5p), and Chromobox 6 (CBX6). The correlation between MIR100HG and clinicopathological features of HCC patients was assessed. Additionally, the effects of MIR100HG knockdown on HCC cell viability, migration, and invasion were explored. The interactions among MIR100HG, miR-146b-5p, and CBX6 were confirmed. Furthermore, rescue experiments were conducted to investigate whether MIR100HG knockdown modulates HCC cell behaviors through modulating the miR-146b-5p/CBX6 axis. RESULTS: The expression of MIR100HG and CBX6 was enhanced, while miR-146b-5p was inhibited in HCC cells. High MIR100HG expression was positively associated with the TNM tumor stage and Edmondson-Steiner grading in HCC patients. MIR100HG knockdown considerably reduced the HCC cell viability, migration, and invasion. In addition, MIR100HG directly targeted miR-146b-5p, and miR-146b-5p directly targeted CBX6 in HCC cells. Moreover, miR-146b-5p suppression or CBX6 elevation evidently rescued the suppressed viability, migration, and invasion of HCC cells caused by MIR100HG knockdown. CONCLUSIONS: Knockdown of MIR100HG inhibited the viability, migration, and invasion of HCC cells by targeting the miR-146b-5p/CBX6 axis, offering a potential therapeutic target for HCC therapy.
format Online
Article
Text
id pubmed-8352691
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-83526912021-08-10 Long Noncoding RNA MIR100HG Knockdown Attenuates Hepatocellular Carcinoma Progression by Regulating MicroRNA-146b-5p/Chromobox 6 Li, Fushun Sun, Xianghua Liu, Qing Liu, Xilu Zhang, Jia Gastroenterol Res Pract Research Article PURPOSE: Hepatocellular carcinoma (HCC) accounts for approximately ninety percent of primary liver cancer. This study attempted to investigate the effects of the long noncoding RNA MIR100HG (MIR100HG) in HCC and the underlying molecular mechanism. MATERIALS AND METHODS: qRT-PCR was implemented to analyze the expression of MIR100HG, microRNA-146b-5p (miR-146b-5p), and Chromobox 6 (CBX6). The correlation between MIR100HG and clinicopathological features of HCC patients was assessed. Additionally, the effects of MIR100HG knockdown on HCC cell viability, migration, and invasion were explored. The interactions among MIR100HG, miR-146b-5p, and CBX6 were confirmed. Furthermore, rescue experiments were conducted to investigate whether MIR100HG knockdown modulates HCC cell behaviors through modulating the miR-146b-5p/CBX6 axis. RESULTS: The expression of MIR100HG and CBX6 was enhanced, while miR-146b-5p was inhibited in HCC cells. High MIR100HG expression was positively associated with the TNM tumor stage and Edmondson-Steiner grading in HCC patients. MIR100HG knockdown considerably reduced the HCC cell viability, migration, and invasion. In addition, MIR100HG directly targeted miR-146b-5p, and miR-146b-5p directly targeted CBX6 in HCC cells. Moreover, miR-146b-5p suppression or CBX6 elevation evidently rescued the suppressed viability, migration, and invasion of HCC cells caused by MIR100HG knockdown. CONCLUSIONS: Knockdown of MIR100HG inhibited the viability, migration, and invasion of HCC cells by targeting the miR-146b-5p/CBX6 axis, offering a potential therapeutic target for HCC therapy. Hindawi 2021-07-30 /pmc/articles/PMC8352691/ /pubmed/34381502 http://dx.doi.org/10.1155/2021/6832518 Text en Copyright © 2021 Fushun Li et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Fushun
Sun, Xianghua
Liu, Qing
Liu, Xilu
Zhang, Jia
Long Noncoding RNA MIR100HG Knockdown Attenuates Hepatocellular Carcinoma Progression by Regulating MicroRNA-146b-5p/Chromobox 6
title Long Noncoding RNA MIR100HG Knockdown Attenuates Hepatocellular Carcinoma Progression by Regulating MicroRNA-146b-5p/Chromobox 6
title_full Long Noncoding RNA MIR100HG Knockdown Attenuates Hepatocellular Carcinoma Progression by Regulating MicroRNA-146b-5p/Chromobox 6
title_fullStr Long Noncoding RNA MIR100HG Knockdown Attenuates Hepatocellular Carcinoma Progression by Regulating MicroRNA-146b-5p/Chromobox 6
title_full_unstemmed Long Noncoding RNA MIR100HG Knockdown Attenuates Hepatocellular Carcinoma Progression by Regulating MicroRNA-146b-5p/Chromobox 6
title_short Long Noncoding RNA MIR100HG Knockdown Attenuates Hepatocellular Carcinoma Progression by Regulating MicroRNA-146b-5p/Chromobox 6
title_sort long noncoding rna mir100hg knockdown attenuates hepatocellular carcinoma progression by regulating microrna-146b-5p/chromobox 6
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8352691/
https://www.ncbi.nlm.nih.gov/pubmed/34381502
http://dx.doi.org/10.1155/2021/6832518
work_keys_str_mv AT lifushun longnoncodingrnamir100hgknockdownattenuateshepatocellularcarcinomaprogressionbyregulatingmicrorna146b5pchromobox6
AT sunxianghua longnoncodingrnamir100hgknockdownattenuateshepatocellularcarcinomaprogressionbyregulatingmicrorna146b5pchromobox6
AT liuqing longnoncodingrnamir100hgknockdownattenuateshepatocellularcarcinomaprogressionbyregulatingmicrorna146b5pchromobox6
AT liuxilu longnoncodingrnamir100hgknockdownattenuateshepatocellularcarcinomaprogressionbyregulatingmicrorna146b5pchromobox6
AT zhangjia longnoncodingrnamir100hgknockdownattenuateshepatocellularcarcinomaprogressionbyregulatingmicrorna146b5pchromobox6