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Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank

One of the main concerns in human milk banks (HMB) is the transmission of human cytomegalovirus (HCMV) that could be present in the milk of infected women. There are consistent data showing that this virus is destroyed by Holder pasteurization (62.5°C for 30 min), but there is a lack of information...

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Autores principales: Gayà, Antoni, Rittà, Massimo, Lembo, David, Tonetto, Paola, Cresi, Francesco, Sottemano, Stefano, Bertino, Enrico, Moro, Guido E., Calvo, Javier, Donalisio, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353116/
https://www.ncbi.nlm.nih.gov/pubmed/34386465
http://dx.doi.org/10.3389/fped.2021.640638
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author Gayà, Antoni
Rittà, Massimo
Lembo, David
Tonetto, Paola
Cresi, Francesco
Sottemano, Stefano
Bertino, Enrico
Moro, Guido E.
Calvo, Javier
Donalisio, Manuela
author_facet Gayà, Antoni
Rittà, Massimo
Lembo, David
Tonetto, Paola
Cresi, Francesco
Sottemano, Stefano
Bertino, Enrico
Moro, Guido E.
Calvo, Javier
Donalisio, Manuela
author_sort Gayà, Antoni
collection PubMed
description One of the main concerns in human milk banks (HMB) is the transmission of human cytomegalovirus (HCMV) that could be present in the milk of infected women. There are consistent data showing that this virus is destroyed by Holder pasteurization (62.5°C for 30 min), but there is a lack of information about the response of the virus to the treatment at lower temperatures in strict HMB conditions. In order to analyze the effectiveness of different temperatures of pasteurization to eliminate HCMV in human milk, a preliminary assay was performed incubating HCMV-spiked raw milk samples from donor mothers at tested temperatures in a PCR thermocycler and the viral infectivity was assayed on cell cultures. No signs of viral replication were observed after treatments at temperatures equal or >53°C for 30, 20, and 10 min, 58°C for 5 min, 59°C for 2 min, and 60°C for 1 min. These data were confirmed in a pasteurizer-like model introducing HCMV-spiked milk in disposable baby bottles. No viral infectivity was detected on cell cultures after heating treatment of milk for 30 min at temperatures from 56 to 60°C. Thus, our results show that by using conventional pasteurization conditions, temperatures in the range of 56–60°C are enough to inactivate HCMV. Consequently, we consider that, in order to provide a higher quality product, the current recommendation to pasteurize both mother's own milk and donated milk at 62.5°C must be re-evaluated.
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spelling pubmed-83531162021-08-11 Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank Gayà, Antoni Rittà, Massimo Lembo, David Tonetto, Paola Cresi, Francesco Sottemano, Stefano Bertino, Enrico Moro, Guido E. Calvo, Javier Donalisio, Manuela Front Pediatr Pediatrics One of the main concerns in human milk banks (HMB) is the transmission of human cytomegalovirus (HCMV) that could be present in the milk of infected women. There are consistent data showing that this virus is destroyed by Holder pasteurization (62.5°C for 30 min), but there is a lack of information about the response of the virus to the treatment at lower temperatures in strict HMB conditions. In order to analyze the effectiveness of different temperatures of pasteurization to eliminate HCMV in human milk, a preliminary assay was performed incubating HCMV-spiked raw milk samples from donor mothers at tested temperatures in a PCR thermocycler and the viral infectivity was assayed on cell cultures. No signs of viral replication were observed after treatments at temperatures equal or >53°C for 30, 20, and 10 min, 58°C for 5 min, 59°C for 2 min, and 60°C for 1 min. These data were confirmed in a pasteurizer-like model introducing HCMV-spiked milk in disposable baby bottles. No viral infectivity was detected on cell cultures after heating treatment of milk for 30 min at temperatures from 56 to 60°C. Thus, our results show that by using conventional pasteurization conditions, temperatures in the range of 56–60°C are enough to inactivate HCMV. Consequently, we consider that, in order to provide a higher quality product, the current recommendation to pasteurize both mother's own milk and donated milk at 62.5°C must be re-evaluated. Frontiers Media S.A. 2021-07-27 /pmc/articles/PMC8353116/ /pubmed/34386465 http://dx.doi.org/10.3389/fped.2021.640638 Text en Copyright © 2021 Gayà, Rittà, Lembo, Tonetto, Cresi, Sottemano, Bertino, Moro, Calvo and Donalisio. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pediatrics
Gayà, Antoni
Rittà, Massimo
Lembo, David
Tonetto, Paola
Cresi, Francesco
Sottemano, Stefano
Bertino, Enrico
Moro, Guido E.
Calvo, Javier
Donalisio, Manuela
Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
title Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
title_full Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
title_fullStr Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
title_full_unstemmed Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
title_short Analysis of Thermal Sensitivity of Human Cytomegalovirus Assayed in the Conventional Conditions of a Human Milk Bank
title_sort analysis of thermal sensitivity of human cytomegalovirus assayed in the conventional conditions of a human milk bank
topic Pediatrics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353116/
https://www.ncbi.nlm.nih.gov/pubmed/34386465
http://dx.doi.org/10.3389/fped.2021.640638
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