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Base Editing in Plants: Applications, Challenges, and Future Prospects

The ability to create targeted modifications in the genomes of plants using genome editing technologies has revolutionized research in crop improvement in the current dispensation of molecular biology. This technology has attracted global attention and has been employed in functional analysis studie...

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Autores principales: Azameti, Mawuli K., Dauda, Wadzani Palnam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353127/
https://www.ncbi.nlm.nih.gov/pubmed/34386023
http://dx.doi.org/10.3389/fpls.2021.664997
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author Azameti, Mawuli K.
Dauda, Wadzani Palnam
author_facet Azameti, Mawuli K.
Dauda, Wadzani Palnam
author_sort Azameti, Mawuli K.
collection PubMed
description The ability to create targeted modifications in the genomes of plants using genome editing technologies has revolutionized research in crop improvement in the current dispensation of molecular biology. This technology has attracted global attention and has been employed in functional analysis studies in crop plants. Since many important agronomic traits are confirmed to be determined by single-nucleotide polymorphisms, improved crop varieties could be developed by the programmed and precise conversion of targeted single bases in the genomes of plants. One novel genome editing approach which serves for this purpose is base editing. Base editing directly makes targeted and irreversible base conversion without creating double-strand breaks (DSBs). This technology has recently gained quick acceptance and adaptation because of its precision, simplicity, and multiplex capabilities. This review focuses on generating different base-editing technologies and how efficient they are in editing nucleic acids. Emphasis is placed on the exploration and applications of these base-editing technologies to enhance crop production. The review also highlights the drawbacks and the prospects of this new technology.
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spelling pubmed-83531272021-08-11 Base Editing in Plants: Applications, Challenges, and Future Prospects Azameti, Mawuli K. Dauda, Wadzani Palnam Front Plant Sci Plant Science The ability to create targeted modifications in the genomes of plants using genome editing technologies has revolutionized research in crop improvement in the current dispensation of molecular biology. This technology has attracted global attention and has been employed in functional analysis studies in crop plants. Since many important agronomic traits are confirmed to be determined by single-nucleotide polymorphisms, improved crop varieties could be developed by the programmed and precise conversion of targeted single bases in the genomes of plants. One novel genome editing approach which serves for this purpose is base editing. Base editing directly makes targeted and irreversible base conversion without creating double-strand breaks (DSBs). This technology has recently gained quick acceptance and adaptation because of its precision, simplicity, and multiplex capabilities. This review focuses on generating different base-editing technologies and how efficient they are in editing nucleic acids. Emphasis is placed on the exploration and applications of these base-editing technologies to enhance crop production. The review also highlights the drawbacks and the prospects of this new technology. Frontiers Media S.A. 2021-07-27 /pmc/articles/PMC8353127/ /pubmed/34386023 http://dx.doi.org/10.3389/fpls.2021.664997 Text en Copyright © 2021 Azameti and Dauda. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Azameti, Mawuli K.
Dauda, Wadzani Palnam
Base Editing in Plants: Applications, Challenges, and Future Prospects
title Base Editing in Plants: Applications, Challenges, and Future Prospects
title_full Base Editing in Plants: Applications, Challenges, and Future Prospects
title_fullStr Base Editing in Plants: Applications, Challenges, and Future Prospects
title_full_unstemmed Base Editing in Plants: Applications, Challenges, and Future Prospects
title_short Base Editing in Plants: Applications, Challenges, and Future Prospects
title_sort base editing in plants: applications, challenges, and future prospects
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353127/
https://www.ncbi.nlm.nih.gov/pubmed/34386023
http://dx.doi.org/10.3389/fpls.2021.664997
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