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Altered Metabolic Phenotype of Immune Cells in a Spontaneous Autoimmune Uveitis Model

As one of the leading causes of blindness worldwide, uveitis is an important disease. The exact pathogenesis of autoimmune uveitis is not entirely elucidated to date. Equine recurrent uveitis (ERU) represents the only spontaneous animal model for autoimmune uveitis in humans. As the metabolism of im...

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Autores principales: Barfüßer, Claudia, Wiedemann, Carmen, Hoffmann, Anne L. C., Hirmer, Sieglinde, Deeg, Cornelia A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353246/
https://www.ncbi.nlm.nih.gov/pubmed/34385998
http://dx.doi.org/10.3389/fimmu.2021.601619
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author Barfüßer, Claudia
Wiedemann, Carmen
Hoffmann, Anne L. C.
Hirmer, Sieglinde
Deeg, Cornelia A.
author_facet Barfüßer, Claudia
Wiedemann, Carmen
Hoffmann, Anne L. C.
Hirmer, Sieglinde
Deeg, Cornelia A.
author_sort Barfüßer, Claudia
collection PubMed
description As one of the leading causes of blindness worldwide, uveitis is an important disease. The exact pathogenesis of autoimmune uveitis is not entirely elucidated to date. Equine recurrent uveitis (ERU) represents the only spontaneous animal model for autoimmune uveitis in humans. As the metabolism of immune cells is an emerging field in research and gains more and more significance to take part in the pathogenesis of various diseases, we conducted experiments to investigate the metabolism of immune cells of ERU cases and healthy controls. To our knowledge, the link between a deviant immunometabolism and the pathogenesis of autoimmune uveitis was not investigated so far. We showed that PBMC of ERU cases had a more active metabolic phenotype in basal state by upregulating both the oxidative phosphorylation and the glycolytic pathway. We further revealed an increased compensatory glycolytic rate of PBMC and CD4(+) T cells of ERU cases under mitochondrial stress conditions. These findings are in line with metabolic alterations of immune cells in other autoimmune diseases and basic research, where it was shown that activated immune cells have an increased need of energy and molecule demand for their effector function. We demonstrated a clear difference in the metabolic phenotypes of PBMC and, more specifically, CD4(+) T cells of ERU cases and controls. These findings are another important step in understanding the pathogenesis of ERU and figuratively, human autoimmune uveitis.
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spelling pubmed-83532462021-08-11 Altered Metabolic Phenotype of Immune Cells in a Spontaneous Autoimmune Uveitis Model Barfüßer, Claudia Wiedemann, Carmen Hoffmann, Anne L. C. Hirmer, Sieglinde Deeg, Cornelia A. Front Immunol Immunology As one of the leading causes of blindness worldwide, uveitis is an important disease. The exact pathogenesis of autoimmune uveitis is not entirely elucidated to date. Equine recurrent uveitis (ERU) represents the only spontaneous animal model for autoimmune uveitis in humans. As the metabolism of immune cells is an emerging field in research and gains more and more significance to take part in the pathogenesis of various diseases, we conducted experiments to investigate the metabolism of immune cells of ERU cases and healthy controls. To our knowledge, the link between a deviant immunometabolism and the pathogenesis of autoimmune uveitis was not investigated so far. We showed that PBMC of ERU cases had a more active metabolic phenotype in basal state by upregulating both the oxidative phosphorylation and the glycolytic pathway. We further revealed an increased compensatory glycolytic rate of PBMC and CD4(+) T cells of ERU cases under mitochondrial stress conditions. These findings are in line with metabolic alterations of immune cells in other autoimmune diseases and basic research, where it was shown that activated immune cells have an increased need of energy and molecule demand for their effector function. We demonstrated a clear difference in the metabolic phenotypes of PBMC and, more specifically, CD4(+) T cells of ERU cases and controls. These findings are another important step in understanding the pathogenesis of ERU and figuratively, human autoimmune uveitis. Frontiers Media S.A. 2021-07-27 /pmc/articles/PMC8353246/ /pubmed/34385998 http://dx.doi.org/10.3389/fimmu.2021.601619 Text en Copyright © 2021 Barfüßer, Wiedemann, Hoffmann, Hirmer and Deeg https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Barfüßer, Claudia
Wiedemann, Carmen
Hoffmann, Anne L. C.
Hirmer, Sieglinde
Deeg, Cornelia A.
Altered Metabolic Phenotype of Immune Cells in a Spontaneous Autoimmune Uveitis Model
title Altered Metabolic Phenotype of Immune Cells in a Spontaneous Autoimmune Uveitis Model
title_full Altered Metabolic Phenotype of Immune Cells in a Spontaneous Autoimmune Uveitis Model
title_fullStr Altered Metabolic Phenotype of Immune Cells in a Spontaneous Autoimmune Uveitis Model
title_full_unstemmed Altered Metabolic Phenotype of Immune Cells in a Spontaneous Autoimmune Uveitis Model
title_short Altered Metabolic Phenotype of Immune Cells in a Spontaneous Autoimmune Uveitis Model
title_sort altered metabolic phenotype of immune cells in a spontaneous autoimmune uveitis model
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353246/
https://www.ncbi.nlm.nih.gov/pubmed/34385998
http://dx.doi.org/10.3389/fimmu.2021.601619
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