ALKBH10B, an mRNA m(6)A Demethylase, Modulates ABA Response During Seed Germination in Arabidopsis

As the most abundant and reversible chemical modification in eukaryotic mRNA, the epitranscriptomic mark N(6)-methyladenine (m(6)A) regulates plant development and stress response. We have previously characterized that ALKBH10B is an Arabidopsis mRNA m(6)A demethylase and regulates floral transition. However, it is unclear whether ALKBH10B plays a role in abiotic stress response. Here, we found that the expression of ALKBH10B is increased in response to abscisic acid (ABA), osmotic, and salt stress. The alkbh10b mutants showed hypersensitive to ABA, osmotic, and salt stress during seed germination. Transcriptome analysis revealed that the expression of several ABA response genes is upregulated in alkbh10b-1 than that of wild type, indicating ALKBH10B negatively affects the ABA signaling. Furthermore, m(6)A sequencing showed that ABA signaling genes, including PYR1, PYL7, PYL9, ABI1, and SnRK2.2 are m(6)A hypermethylated in alkbh10b-1 after ABA treatment. Taken together, our work demonstrated that ALKBH10B negatively modulates ABA response during seed germination in Arabidopsis.