Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration

Fever is a complex physiological response enhancing immune surveillance during infection and inflammation. Fever-range whole-body hyperthermia (WBH) treatment can experimentally mimic the febrile condition in mice. Here, we describe a protocol for the treatment of mice with WBH and normothermia. We...

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Detalles Bibliográficos
Autores principales: Lin, ChangDong, Liu, ZhaoYuan, Li, Yue, Chen, JianFeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353337/
https://www.ncbi.nlm.nih.gov/pubmed/34401786
http://dx.doi.org/10.1016/j.xpro.2021.100720
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author Lin, ChangDong
Liu, ZhaoYuan
Li, Yue
Chen, JianFeng
author_facet Lin, ChangDong
Liu, ZhaoYuan
Li, Yue
Chen, JianFeng
author_sort Lin, ChangDong
collection PubMed
description Fever is a complex physiological response enhancing immune surveillance during infection and inflammation. Fever-range whole-body hyperthermia (WBH) treatment can experimentally mimic the febrile condition in mice. Here, we describe a protocol for the treatment of mice with WBH and normothermia. We describe the isolation of T cells from mouse spleen followed by the evaluation of T-cell adhesion and transmigration. This animal model can be applied to studying the dysfunction of the immune system induced by fever. For complete details on the use and execution of this protocol, please refer to Lin et al. (2019).
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spelling pubmed-83533372021-08-15 Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration Lin, ChangDong Liu, ZhaoYuan Li, Yue Chen, JianFeng STAR Protoc Protocol Fever is a complex physiological response enhancing immune surveillance during infection and inflammation. Fever-range whole-body hyperthermia (WBH) treatment can experimentally mimic the febrile condition in mice. Here, we describe a protocol for the treatment of mice with WBH and normothermia. We describe the isolation of T cells from mouse spleen followed by the evaluation of T-cell adhesion and transmigration. This animal model can be applied to studying the dysfunction of the immune system induced by fever. For complete details on the use and execution of this protocol, please refer to Lin et al. (2019). Elsevier 2021-08-05 /pmc/articles/PMC8353337/ /pubmed/34401786 http://dx.doi.org/10.1016/j.xpro.2021.100720 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lin, ChangDong
Liu, ZhaoYuan
Li, Yue
Chen, JianFeng
Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration
title Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration
title_full Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration
title_fullStr Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration
title_full_unstemmed Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration
title_short Protocol for fever-range whole-body hyperthermia (WBH) in mice to study febrile effect on T-cell adhesion and migration
title_sort protocol for fever-range whole-body hyperthermia (wbh) in mice to study febrile effect on t-cell adhesion and migration
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353337/
https://www.ncbi.nlm.nih.gov/pubmed/34401786
http://dx.doi.org/10.1016/j.xpro.2021.100720
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