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Collection of cells for single-cell RNA sequencing using high-resolution fluorescence microscopy

FACS sorting followed by single-cell RNA-sequencing (SORT-Seq) is a popular procedure to select cells of interest for single-cell transcriptomics. However, FACS is not suitable for measurement of subcellular distribution of fluorescence or for small samples (<1,000 cells). The VYCAP puncher syste...

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Detalles Bibliográficos
Autores principales:	Segeren, Hendrika A., Andree, Kiki C., Oomens, Lisa, Westendorp, Bart
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353340/ https://www.ncbi.nlm.nih.gov/pubmed/34401784 http://dx.doi.org/10.1016/j.xpro.2021.100718

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