Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM)

Disruption of mitochondrial morphology occurs during various diseases, but the biological significance is not entirely clear. Here, we describe a detailed step-by-step protocol for a chemically inducible dimerization system-based synthetic protein device, termed inducible counter mitochondrial morph...

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Detalles Bibliográficos
Autores principales: Miyamoto, Takafumi, Han, Song-Iee, Shimano, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353406/
https://www.ncbi.nlm.nih.gov/pubmed/34401787
http://dx.doi.org/10.1016/j.xpro.2021.100721
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author Miyamoto, Takafumi
Han, Song-Iee
Shimano, Hitoshi
author_facet Miyamoto, Takafumi
Han, Song-Iee
Shimano, Hitoshi
author_sort Miyamoto, Takafumi
collection PubMed
description Disruption of mitochondrial morphology occurs during various diseases, but the biological significance is not entirely clear. Here, we describe a detailed step-by-step protocol for a chemically inducible dimerization system-based synthetic protein device, termed inducible counter mitochondrial morphology. This system allows artificial manipulation of mitochondrial morphology on a timescale of minutes in living mammalian cells. We also describe an AI-assisted imaging processing approach. For complete details on the use and execution of this protocol, please refer to Miyamoto et al., 2021.
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spelling pubmed-83534062021-08-15 Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM) Miyamoto, Takafumi Han, Song-Iee Shimano, Hitoshi STAR Protoc Protocol Disruption of mitochondrial morphology occurs during various diseases, but the biological significance is not entirely clear. Here, we describe a detailed step-by-step protocol for a chemically inducible dimerization system-based synthetic protein device, termed inducible counter mitochondrial morphology. This system allows artificial manipulation of mitochondrial morphology on a timescale of minutes in living mammalian cells. We also describe an AI-assisted imaging processing approach. For complete details on the use and execution of this protocol, please refer to Miyamoto et al., 2021. Elsevier 2021-08-05 /pmc/articles/PMC8353406/ /pubmed/34401787 http://dx.doi.org/10.1016/j.xpro.2021.100721 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Miyamoto, Takafumi
Han, Song-Iee
Shimano, Hitoshi
Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM)
title Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM)
title_full Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM)
title_fullStr Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM)
title_full_unstemmed Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM)
title_short Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (iCMM)
title_sort protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology (icmm)
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353406/
https://www.ncbi.nlm.nih.gov/pubmed/34401787
http://dx.doi.org/10.1016/j.xpro.2021.100721
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