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Molecular study of metallo-β-lactamases and integrons in Acinetobacter baumannii isolates from burn patients
BACKGROUND: Productions of metallo-β-lactamases enzymes are the most common mechanism of antibiotic resistance to all beta-lactam classes (except monobactams) in Acinetobacter baumannii. MBLs are usually associated with gene cassettes of integrons and spread easily among bacteria. The current study...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353788/ https://www.ncbi.nlm.nih.gov/pubmed/34372787 http://dx.doi.org/10.1186/s12879-021-06513-w |
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author | Nikibakhsh, Mahnaz Firoozeh, Farzaneh Badmasti, Farzad Kabir, Kourosh Zibaei, Mohammad |
author_facet | Nikibakhsh, Mahnaz Firoozeh, Farzaneh Badmasti, Farzad Kabir, Kourosh Zibaei, Mohammad |
author_sort | Nikibakhsh, Mahnaz |
collection | PubMed |
description | BACKGROUND: Productions of metallo-β-lactamases enzymes are the most common mechanism of antibiotic resistance to all beta-lactam classes (except monobactams) in Acinetobacter baumannii. MBLs are usually associated with gene cassettes of integrons and spread easily among bacteria. The current study was performed to detect the genes encoding MBLs and integron structures in A. baumannii isolates from burn patients. METHODS: This study was performed on 106 non-duplicate A. baumannii isolates from burn patients referred to Shahid Motahari Hospital in Tehran. Antibiotic susceptibility of A. baumannii isolates was performed using disk diffusion and broth microdilution method in accordance with the CLSI guidelines. The presence of class 1 integron and associated gene cassettes as well as MBLs-encoding genes including bla(VIM), and bla(IMP) were investigated using PCR and sequencing techniques. RESULTS: In this cross-sectional study all (100%) of the A. baumannii isolates examined were multidrug resistant. All isolates were sensitive to colistin and simultaneously all were resistant to imipenem. PCR assays showed the presence of bla(VIM) and bla(IMP) genes in 102 (96.2%) and 62 (58.5%) isolates of A. baumannii respectively. In addition, 62 (58.5%) of the A. baumannii isolates carried integron class 1, of which 49 (79.0%) were identified with at least one gene cassette. Three types of integron class 1 gene cassettes were identified including: arr2, cmlA5, qacE1 (2300 bp); arr-2, ereC, aadA1, cmlA7, qacE1 (4800 bp); and aac(3)-Ic, cmlA5 (2250 bp). CONCLUSION: A high prevalence of MBLs genes, especially bla(VIM), was identified in the studied MDR A. baumannii isolates. In addition, most of the strains carried class 1 integrons. Furthermore, the gene cassettes arrays of integrons including cmlA5 and cmlA7 were detected, for the first time, in A. baumannii strains in Iran. |
format | Online Article Text |
id | pubmed-8353788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-83537882021-08-10 Molecular study of metallo-β-lactamases and integrons in Acinetobacter baumannii isolates from burn patients Nikibakhsh, Mahnaz Firoozeh, Farzaneh Badmasti, Farzad Kabir, Kourosh Zibaei, Mohammad BMC Infect Dis Research BACKGROUND: Productions of metallo-β-lactamases enzymes are the most common mechanism of antibiotic resistance to all beta-lactam classes (except monobactams) in Acinetobacter baumannii. MBLs are usually associated with gene cassettes of integrons and spread easily among bacteria. The current study was performed to detect the genes encoding MBLs and integron structures in A. baumannii isolates from burn patients. METHODS: This study was performed on 106 non-duplicate A. baumannii isolates from burn patients referred to Shahid Motahari Hospital in Tehran. Antibiotic susceptibility of A. baumannii isolates was performed using disk diffusion and broth microdilution method in accordance with the CLSI guidelines. The presence of class 1 integron and associated gene cassettes as well as MBLs-encoding genes including bla(VIM), and bla(IMP) were investigated using PCR and sequencing techniques. RESULTS: In this cross-sectional study all (100%) of the A. baumannii isolates examined were multidrug resistant. All isolates were sensitive to colistin and simultaneously all were resistant to imipenem. PCR assays showed the presence of bla(VIM) and bla(IMP) genes in 102 (96.2%) and 62 (58.5%) isolates of A. baumannii respectively. In addition, 62 (58.5%) of the A. baumannii isolates carried integron class 1, of which 49 (79.0%) were identified with at least one gene cassette. Three types of integron class 1 gene cassettes were identified including: arr2, cmlA5, qacE1 (2300 bp); arr-2, ereC, aadA1, cmlA7, qacE1 (4800 bp); and aac(3)-Ic, cmlA5 (2250 bp). CONCLUSION: A high prevalence of MBLs genes, especially bla(VIM), was identified in the studied MDR A. baumannii isolates. In addition, most of the strains carried class 1 integrons. Furthermore, the gene cassettes arrays of integrons including cmlA5 and cmlA7 were detected, for the first time, in A. baumannii strains in Iran. BioMed Central 2021-08-09 /pmc/articles/PMC8353788/ /pubmed/34372787 http://dx.doi.org/10.1186/s12879-021-06513-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Nikibakhsh, Mahnaz Firoozeh, Farzaneh Badmasti, Farzad Kabir, Kourosh Zibaei, Mohammad Molecular study of metallo-β-lactamases and integrons in Acinetobacter baumannii isolates from burn patients |
title | Molecular study of metallo-β-lactamases and integrons in Acinetobacter baumannii isolates from burn patients |
title_full | Molecular study of metallo-β-lactamases and integrons in Acinetobacter baumannii isolates from burn patients |
title_fullStr | Molecular study of metallo-β-lactamases and integrons in Acinetobacter baumannii isolates from burn patients |
title_full_unstemmed | Molecular study of metallo-β-lactamases and integrons in Acinetobacter baumannii isolates from burn patients |
title_short | Molecular study of metallo-β-lactamases and integrons in Acinetobacter baumannii isolates from burn patients |
title_sort | molecular study of metallo-β-lactamases and integrons in acinetobacter baumannii isolates from burn patients |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353788/ https://www.ncbi.nlm.nih.gov/pubmed/34372787 http://dx.doi.org/10.1186/s12879-021-06513-w |
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