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Mycobacterium tuberculosis Specific Protein Rv1509 Evokes Efficient Innate and Adaptive Immune Response Indicative of Protective Th1 Immune Signature

Dissecting the function(s) of proteins present exclusively in Mycobacterium tuberculosis (M.tb) will provide important clues regarding the role of these proteins in mycobacterial pathogenesis. Using extensive computational approaches, we shortlisted ORFs/proteins unique to M.tb among 13 different sp...

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Autores principales: P, Manjunath, Ahmad, Javeed, Samal, Jasmine, Sheikh, Javaid Ahmad, Arora, Simran Kaur, Khubaib, Mohd, Aggarwal, Heena, Kumari, Indu, Luthra, Kalpana, Rahman, Syed Asad, Hasnain, Seyed E., Ehtesham, Nasreen Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354026/
https://www.ncbi.nlm.nih.gov/pubmed/34386011
http://dx.doi.org/10.3389/fimmu.2021.706081
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author P, Manjunath
Ahmad, Javeed
Samal, Jasmine
Sheikh, Javaid Ahmad
Arora, Simran Kaur
Khubaib, Mohd
Aggarwal, Heena
Kumari, Indu
Luthra, Kalpana
Rahman, Syed Asad
Hasnain, Seyed E.
Ehtesham, Nasreen Z.
author_facet P, Manjunath
Ahmad, Javeed
Samal, Jasmine
Sheikh, Javaid Ahmad
Arora, Simran Kaur
Khubaib, Mohd
Aggarwal, Heena
Kumari, Indu
Luthra, Kalpana
Rahman, Syed Asad
Hasnain, Seyed E.
Ehtesham, Nasreen Z.
author_sort P, Manjunath
collection PubMed
description Dissecting the function(s) of proteins present exclusively in Mycobacterium tuberculosis (M.tb) will provide important clues regarding the role of these proteins in mycobacterial pathogenesis. Using extensive computational approaches, we shortlisted ORFs/proteins unique to M.tb among 13 different species of mycobacteria and identified a hypothetical protein Rv1509 as a ‘signature protein’ of M.tb. This unique protein was found to be present only in M.tb and absent in all other mycobacterial species, including BCG. In silico analysis identified numerous putative T cell and B cell epitopes in Rv1509. Initial in vitro experiments using innate immune cells demonstrated Rv1509 to be immunogenic with potential to modulate innate immune responses. Macrophages treated with Rv1509 exhibited higher activation status along with substantial release of pro-inflammatory cytokines. Besides, Rv1509 protein boosts dendritic cell maturation by increasing the expression of activation markers such as CD80, HLA-DR and decreasing DC-SIGN expression and this interaction was mediated by innate immune receptor TLR2. Further, in vivo experiments in mice demonstrated that Rv1509 protein promotes the expansion of multifunctional CD4+ and CD8+T cells and induces effector memory response along with evoking a canonical Th1 type of immune response. Rv1509 also induces substantial B cell response as revealed by increased IgG reactivity in sera of immunized animals. This allowed us to demonstrate the diagnostic efficacy of this protein in sera of human TB patients compared to the healthy controls. Taken together, our results reveal that Rv1509 signature protein has immunomodulatory functions evoking immunological memory response with possible implications in serodiagnosis and TB vaccine development.
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spelling pubmed-83540262021-08-11 Mycobacterium tuberculosis Specific Protein Rv1509 Evokes Efficient Innate and Adaptive Immune Response Indicative of Protective Th1 Immune Signature P, Manjunath Ahmad, Javeed Samal, Jasmine Sheikh, Javaid Ahmad Arora, Simran Kaur Khubaib, Mohd Aggarwal, Heena Kumari, Indu Luthra, Kalpana Rahman, Syed Asad Hasnain, Seyed E. Ehtesham, Nasreen Z. Front Immunol Immunology Dissecting the function(s) of proteins present exclusively in Mycobacterium tuberculosis (M.tb) will provide important clues regarding the role of these proteins in mycobacterial pathogenesis. Using extensive computational approaches, we shortlisted ORFs/proteins unique to M.tb among 13 different species of mycobacteria and identified a hypothetical protein Rv1509 as a ‘signature protein’ of M.tb. This unique protein was found to be present only in M.tb and absent in all other mycobacterial species, including BCG. In silico analysis identified numerous putative T cell and B cell epitopes in Rv1509. Initial in vitro experiments using innate immune cells demonstrated Rv1509 to be immunogenic with potential to modulate innate immune responses. Macrophages treated with Rv1509 exhibited higher activation status along with substantial release of pro-inflammatory cytokines. Besides, Rv1509 protein boosts dendritic cell maturation by increasing the expression of activation markers such as CD80, HLA-DR and decreasing DC-SIGN expression and this interaction was mediated by innate immune receptor TLR2. Further, in vivo experiments in mice demonstrated that Rv1509 protein promotes the expansion of multifunctional CD4+ and CD8+T cells and induces effector memory response along with evoking a canonical Th1 type of immune response. Rv1509 also induces substantial B cell response as revealed by increased IgG reactivity in sera of immunized animals. This allowed us to demonstrate the diagnostic efficacy of this protein in sera of human TB patients compared to the healthy controls. Taken together, our results reveal that Rv1509 signature protein has immunomodulatory functions evoking immunological memory response with possible implications in serodiagnosis and TB vaccine development. Frontiers Media S.A. 2021-07-27 /pmc/articles/PMC8354026/ /pubmed/34386011 http://dx.doi.org/10.3389/fimmu.2021.706081 Text en Copyright © 2021 P, Ahmad, Samal, Sheikh, Arora, Khubaib, Aggarwal, Kumari, Luthra, Rahman, Hasnain and Ehtesham https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
P, Manjunath
Ahmad, Javeed
Samal, Jasmine
Sheikh, Javaid Ahmad
Arora, Simran Kaur
Khubaib, Mohd
Aggarwal, Heena
Kumari, Indu
Luthra, Kalpana
Rahman, Syed Asad
Hasnain, Seyed E.
Ehtesham, Nasreen Z.
Mycobacterium tuberculosis Specific Protein Rv1509 Evokes Efficient Innate and Adaptive Immune Response Indicative of Protective Th1 Immune Signature
title Mycobacterium tuberculosis Specific Protein Rv1509 Evokes Efficient Innate and Adaptive Immune Response Indicative of Protective Th1 Immune Signature
title_full Mycobacterium tuberculosis Specific Protein Rv1509 Evokes Efficient Innate and Adaptive Immune Response Indicative of Protective Th1 Immune Signature
title_fullStr Mycobacterium tuberculosis Specific Protein Rv1509 Evokes Efficient Innate and Adaptive Immune Response Indicative of Protective Th1 Immune Signature
title_full_unstemmed Mycobacterium tuberculosis Specific Protein Rv1509 Evokes Efficient Innate and Adaptive Immune Response Indicative of Protective Th1 Immune Signature
title_short Mycobacterium tuberculosis Specific Protein Rv1509 Evokes Efficient Innate and Adaptive Immune Response Indicative of Protective Th1 Immune Signature
title_sort mycobacterium tuberculosis specific protein rv1509 evokes efficient innate and adaptive immune response indicative of protective th1 immune signature
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354026/
https://www.ncbi.nlm.nih.gov/pubmed/34386011
http://dx.doi.org/10.3389/fimmu.2021.706081
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