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Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica)

The Australian finger lime is a unique citrus species that has gained importance due to its unique fruit characteristics and perceived tolerance to Huanglongbing (HLB), an often-fatal disease of citrus trees. In this study, we developed allotetraploid finger lime hybrids and cybrids by utilizing som...

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Autores principales: Dutt, Manjul, Mahmoud, Lamiaa M., Chamusco, Karen, Stanton, Daniel, Chase, Christine D., Nielsen, Ethan, Quirico, Maria, Yu, Qibin, Gmitter, Frederick G., Grosser, Jude W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354479/
https://www.ncbi.nlm.nih.gov/pubmed/34375348
http://dx.doi.org/10.1371/journal.pone.0255842
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author Dutt, Manjul
Mahmoud, Lamiaa M.
Chamusco, Karen
Stanton, Daniel
Chase, Christine D.
Nielsen, Ethan
Quirico, Maria
Yu, Qibin
Gmitter, Frederick G.
Grosser, Jude W.
author_facet Dutt, Manjul
Mahmoud, Lamiaa M.
Chamusco, Karen
Stanton, Daniel
Chase, Christine D.
Nielsen, Ethan
Quirico, Maria
Yu, Qibin
Gmitter, Frederick G.
Grosser, Jude W.
author_sort Dutt, Manjul
collection PubMed
description The Australian finger lime is a unique citrus species that has gained importance due to its unique fruit characteristics and perceived tolerance to Huanglongbing (HLB), an often-fatal disease of citrus trees. In this study, we developed allotetraploid finger lime hybrids and cybrids by utilizing somatic cell fusion techniques to fuse diploid ‘OLL8’ sweet orange or ‘Page’ tangelo callus-derived protoplasts with finger lime (FL) mesophyll-derived protoplasts. Six somatic fusions were regenerated from the ‘OLL8’ + FL fusion, while three putative cybrids were regenerated from the ‘Page’ + FL fusion. Ploidy levels and nuclear-expressed sequence tag derived simple sequence repeat (EST-SSR) markers confirmed the somatic hybrid production, and mitochondrial DNA primer sets confirmed the cybrid nature. Several trees produced by the somatic fusion remained HLB negative even after 6 years of growth in an HLB-endemic environment. Pathogenesis related (PR) and other genes that are often upregulated in HLB-tolerant trees were also upregulated in our somatic fusions. These newly developed somatic fusions and cybrids could potentially be used as breeding parents to develop the next generation of improved HLB-tolerant rootstocks and scions.
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spelling pubmed-83544792021-08-11 Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica) Dutt, Manjul Mahmoud, Lamiaa M. Chamusco, Karen Stanton, Daniel Chase, Christine D. Nielsen, Ethan Quirico, Maria Yu, Qibin Gmitter, Frederick G. Grosser, Jude W. PLoS One Research Article The Australian finger lime is a unique citrus species that has gained importance due to its unique fruit characteristics and perceived tolerance to Huanglongbing (HLB), an often-fatal disease of citrus trees. In this study, we developed allotetraploid finger lime hybrids and cybrids by utilizing somatic cell fusion techniques to fuse diploid ‘OLL8’ sweet orange or ‘Page’ tangelo callus-derived protoplasts with finger lime (FL) mesophyll-derived protoplasts. Six somatic fusions were regenerated from the ‘OLL8’ + FL fusion, while three putative cybrids were regenerated from the ‘Page’ + FL fusion. Ploidy levels and nuclear-expressed sequence tag derived simple sequence repeat (EST-SSR) markers confirmed the somatic hybrid production, and mitochondrial DNA primer sets confirmed the cybrid nature. Several trees produced by the somatic fusion remained HLB negative even after 6 years of growth in an HLB-endemic environment. Pathogenesis related (PR) and other genes that are often upregulated in HLB-tolerant trees were also upregulated in our somatic fusions. These newly developed somatic fusions and cybrids could potentially be used as breeding parents to develop the next generation of improved HLB-tolerant rootstocks and scions. Public Library of Science 2021-08-10 /pmc/articles/PMC8354479/ /pubmed/34375348 http://dx.doi.org/10.1371/journal.pone.0255842 Text en © 2021 Dutt et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dutt, Manjul
Mahmoud, Lamiaa M.
Chamusco, Karen
Stanton, Daniel
Chase, Christine D.
Nielsen, Ethan
Quirico, Maria
Yu, Qibin
Gmitter, Frederick G.
Grosser, Jude W.
Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica)
title Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica)
title_full Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica)
title_fullStr Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica)
title_full_unstemmed Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica)
title_short Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica)
title_sort utilization of somatic fusion techniques for the development of hlb tolerant breeding resources employing the australian finger lime (citrus australasica)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354479/
https://www.ncbi.nlm.nih.gov/pubmed/34375348
http://dx.doi.org/10.1371/journal.pone.0255842
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