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CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy
OBJECTIVE: The role of cell death‐inducing DFF45‐like effector C (CIDEC) in insulin resistance has been established, and it is considered to be an important trigger factor for the progression of diabetic nephropathy (DN). We intend to explore whether CIDEC plays an important role in the regulation o...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354488/ https://www.ncbi.nlm.nih.gov/pubmed/33655702 http://dx.doi.org/10.1111/jdi.13534 |
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author | Zheng, Gao‐shu Tan, Yan‐min Shang, Yuan‐yuan Liu, Ya‐peng Hu, Bo‐ang Wang, Di Han, Lu Wang, Zhi‐hao Zhang, Wei Ti, Yun Zhong, Ming |
author_facet | Zheng, Gao‐shu Tan, Yan‐min Shang, Yuan‐yuan Liu, Ya‐peng Hu, Bo‐ang Wang, Di Han, Lu Wang, Zhi‐hao Zhang, Wei Ti, Yun Zhong, Ming |
author_sort | Zheng, Gao‐shu |
collection | PubMed |
description | OBJECTIVE: The role of cell death‐inducing DFF45‐like effector C (CIDEC) in insulin resistance has been established, and it is considered to be an important trigger factor for the progression of diabetic nephropathy (DN). We intend to explore whether CIDEC plays an important role in the regulation of DN and its potential mechanism. METHODS: High‐fat diet and low dose streptozotocin were used to establish type 2 diabetic rat model. We investigate the role of CIDEC in the pathogenesis and process of DN through histopathological analysis, western blot and gene silencing. Meanwhile, the effect of CIDEC on renal tubular epithelial cells stimulated by high glucose was also verified. RESULTS: DM group exhibited glucose and lipid metabolic disturbance, with hypertrophy of kidneys, damaged renal function, increased apoptosis, decreased autophagy, glomerulosclerosis and interstitial fibrosis. CIDEC gene silencing improved metabolic disorder and insulin resistance, alleviated renal hypertrophy and renal function damage, decreased glomerular and tubular apoptosis, increased autophagy and inhibited renal fibrosis. At the cellular level, high glucose stimulation increased CIDEC expression in renal tubular epithelial cells, accompanied by increased apoptosis and decreased autophagy. CIDEC gene silencing can improve autophagy and reduce apoptosis. At the molecular level, CIDEC gene silencing also decreased the expression of early growth response factor (EGR)1 and increased the expression of adipose triglyceride lipase (ATGL). CONCLUSION: CIDEC gene silencing may delay the progression of DN by restoring autophagy activity and inhibiting apoptosis with the participation of EGR1and ATGL. |
format | Online Article Text |
id | pubmed-8354488 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83544882021-08-15 CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy Zheng, Gao‐shu Tan, Yan‐min Shang, Yuan‐yuan Liu, Ya‐peng Hu, Bo‐ang Wang, Di Han, Lu Wang, Zhi‐hao Zhang, Wei Ti, Yun Zhong, Ming J Diabetes Investig Articles OBJECTIVE: The role of cell death‐inducing DFF45‐like effector C (CIDEC) in insulin resistance has been established, and it is considered to be an important trigger factor for the progression of diabetic nephropathy (DN). We intend to explore whether CIDEC plays an important role in the regulation of DN and its potential mechanism. METHODS: High‐fat diet and low dose streptozotocin were used to establish type 2 diabetic rat model. We investigate the role of CIDEC in the pathogenesis and process of DN through histopathological analysis, western blot and gene silencing. Meanwhile, the effect of CIDEC on renal tubular epithelial cells stimulated by high glucose was also verified. RESULTS: DM group exhibited glucose and lipid metabolic disturbance, with hypertrophy of kidneys, damaged renal function, increased apoptosis, decreased autophagy, glomerulosclerosis and interstitial fibrosis. CIDEC gene silencing improved metabolic disorder and insulin resistance, alleviated renal hypertrophy and renal function damage, decreased glomerular and tubular apoptosis, increased autophagy and inhibited renal fibrosis. At the cellular level, high glucose stimulation increased CIDEC expression in renal tubular epithelial cells, accompanied by increased apoptosis and decreased autophagy. CIDEC gene silencing can improve autophagy and reduce apoptosis. At the molecular level, CIDEC gene silencing also decreased the expression of early growth response factor (EGR)1 and increased the expression of adipose triglyceride lipase (ATGL). CONCLUSION: CIDEC gene silencing may delay the progression of DN by restoring autophagy activity and inhibiting apoptosis with the participation of EGR1and ATGL. John Wiley and Sons Inc. 2021-08-10 2021-08 /pmc/articles/PMC8354488/ /pubmed/33655702 http://dx.doi.org/10.1111/jdi.13534 Text en © 2021 The Authors. Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zheng, Gao‐shu Tan, Yan‐min Shang, Yuan‐yuan Liu, Ya‐peng Hu, Bo‐ang Wang, Di Han, Lu Wang, Zhi‐hao Zhang, Wei Ti, Yun Zhong, Ming CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy |
title | CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy |
title_full | CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy |
title_fullStr | CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy |
title_full_unstemmed | CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy |
title_short | CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy |
title_sort | cidec silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354488/ https://www.ncbi.nlm.nih.gov/pubmed/33655702 http://dx.doi.org/10.1111/jdi.13534 |
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