Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing

Clostridium botulinum group III is the anaerobic Gram-positive bacterium producing the deadly neurotoxin responsible for animal botulism. Here, we used long-read sequencing to produce four complete genomes from Clostridium botulinum group III neurotoxin types C, D, C/D, and D/C. The protocol for obt...

Descripción completa

Detalles Bibliográficos
Autores principales: Woudstra, Cedric, Mäklin, Tommi, Derman, Yagmur, Bano, Luca, Skarin, Hanna, Mazuet, Christelle, Honkela, Antti, Lindström, Miia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Genome Sequences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354527/
https://www.ncbi.nlm.nih.gov/pubmed/34080898
http://dx.doi.org/10.1128/MRA.01364-20
_version_ 1783736612276928512
author Woudstra, Cedric
Mäklin, Tommi
Derman, Yagmur
Bano, Luca
Skarin, Hanna
Mazuet, Christelle
Honkela, Antti
Lindström, Miia
author_facet Woudstra, Cedric
Mäklin, Tommi
Derman, Yagmur
Bano, Luca
Skarin, Hanna
Mazuet, Christelle
Honkela, Antti
Lindström, Miia
author_sort Woudstra, Cedric
collection PubMed
description Clostridium botulinum group III is the anaerobic Gram-positive bacterium producing the deadly neurotoxin responsible for animal botulism. Here, we used long-read sequencing to produce four complete genomes from Clostridium botulinum group III neurotoxin types C, D, C/D, and D/C. The protocol for obtaining high-molecular-weight DNA from C. botulinum group III is described.
format Online
Article
Text
id pubmed-8354527
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-83545272021-08-13 Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing Woudstra, Cedric Mäklin, Tommi Derman, Yagmur Bano, Luca Skarin, Hanna Mazuet, Christelle Honkela, Antti Lindström, Miia Microbiol Resour Announc Genome Sequences Clostridium botulinum group III is the anaerobic Gram-positive bacterium producing the deadly neurotoxin responsible for animal botulism. Here, we used long-read sequencing to produce four complete genomes from Clostridium botulinum group III neurotoxin types C, D, C/D, and D/C. The protocol for obtaining high-molecular-weight DNA from C. botulinum group III is described. American Society for Microbiology 2021-06-03 /pmc/articles/PMC8354527/ /pubmed/34080898 http://dx.doi.org/10.1128/MRA.01364-20 Text en Copyright © 2021 Woudstra et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Genome Sequences
Woudstra, Cedric
Mäklin, Tommi
Derman, Yagmur
Bano, Luca
Skarin, Hanna
Mazuet, Christelle
Honkela, Antti
Lindström, Miia
Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing
title Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing
title_full Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing
title_fullStr Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing
title_full_unstemmed Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing
title_short Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing
title_sort closing clostridium botulinum group iii genomes using long-read sequencing
topic Genome Sequences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354527/
https://www.ncbi.nlm.nih.gov/pubmed/34080898
http://dx.doi.org/10.1128/MRA.01364-20
work_keys_str_mv AT woudstracedric closingclostridiumbotulinumgroupiiigenomesusinglongreadsequencing
AT maklintommi closingclostridiumbotulinumgroupiiigenomesusinglongreadsequencing
AT dermanyagmur closingclostridiumbotulinumgroupiiigenomesusinglongreadsequencing
AT banoluca closingclostridiumbotulinumgroupiiigenomesusinglongreadsequencing
AT skarinhanna closingclostridiumbotulinumgroupiiigenomesusinglongreadsequencing
AT mazuetchristelle closingclostridiumbotulinumgroupiiigenomesusinglongreadsequencing
AT honkelaantti closingclostridiumbotulinumgroupiiigenomesusinglongreadsequencing
AT lindstrommiia closingclostridiumbotulinumgroupiiigenomesusinglongreadsequencing

Ejemplares similares