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Genome-Wide Identification and Characterization of the Potato IQD Family During Development and Stress

Calmodulin-binding proteins belong to the IQ67 domain (IQD) gene family and play essential roles in plant development and stress responses. However, the role of IQD gene family in potato (Solanum tuberosum L.) is yet to be known. In the present study, 23 StIQDs were identified in the potato genome a...

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Autores principales: Mei, Chao, Liu, Yuwei, Dong, Xue, Song, Qianna, Wang, Huijie, Shi, Hongwei, Feng, Ruiyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354571/
https://www.ncbi.nlm.nih.gov/pubmed/34386041
http://dx.doi.org/10.3389/fgene.2021.693936
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author Mei, Chao
Liu, Yuwei
Dong, Xue
Song, Qianna
Wang, Huijie
Shi, Hongwei
Feng, Ruiyun
author_facet Mei, Chao
Liu, Yuwei
Dong, Xue
Song, Qianna
Wang, Huijie
Shi, Hongwei
Feng, Ruiyun
author_sort Mei, Chao
collection PubMed
description Calmodulin-binding proteins belong to the IQ67 domain (IQD) gene family and play essential roles in plant development and stress responses. However, the role of IQD gene family in potato (Solanum tuberosum L.) is yet to be known. In the present study, 23 StIQDs were identified in the potato genome and named StIQD1 to StIQD23. They were unevenly distributed on 10 of the 12 chromosomes. Phylogenetic analysis divided the IQDs into four subfamilies (IQD I–IV). StIQDs found in three of the four subfamilies. Synteny analysis confirmed that potato and tomato shared a close evolutionary relationship. Besides, RNA-Seq data analysis revealed that the expression of 19 of the 23 StIQDs was detected in at least one of the 12 tissues, and some of which showed a tissue-specific pattern. Quantitative reverse transcriptase–polymerase chain reaction results further confirmed that 14 StIQDs responded differently to various abiotic stresses, including drought, extreme temperature, and CaCl(2) treatment, suggesting their significance in stress response. This study presents a comprehensive overview of the potato IQD gene family and lays a foundation for further analysis of the StIQDs functions in plant development and stress response.
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spelling pubmed-83545712021-08-11 Genome-Wide Identification and Characterization of the Potato IQD Family During Development and Stress Mei, Chao Liu, Yuwei Dong, Xue Song, Qianna Wang, Huijie Shi, Hongwei Feng, Ruiyun Front Genet Genetics Calmodulin-binding proteins belong to the IQ67 domain (IQD) gene family and play essential roles in plant development and stress responses. However, the role of IQD gene family in potato (Solanum tuberosum L.) is yet to be known. In the present study, 23 StIQDs were identified in the potato genome and named StIQD1 to StIQD23. They were unevenly distributed on 10 of the 12 chromosomes. Phylogenetic analysis divided the IQDs into four subfamilies (IQD I–IV). StIQDs found in three of the four subfamilies. Synteny analysis confirmed that potato and tomato shared a close evolutionary relationship. Besides, RNA-Seq data analysis revealed that the expression of 19 of the 23 StIQDs was detected in at least one of the 12 tissues, and some of which showed a tissue-specific pattern. Quantitative reverse transcriptase–polymerase chain reaction results further confirmed that 14 StIQDs responded differently to various abiotic stresses, including drought, extreme temperature, and CaCl(2) treatment, suggesting their significance in stress response. This study presents a comprehensive overview of the potato IQD gene family and lays a foundation for further analysis of the StIQDs functions in plant development and stress response. Frontiers Media S.A. 2021-07-27 /pmc/articles/PMC8354571/ /pubmed/34386041 http://dx.doi.org/10.3389/fgene.2021.693936 Text en Copyright © 2021 Mei, Liu, Dong, Song, Wang, Shi and Feng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Mei, Chao
Liu, Yuwei
Dong, Xue
Song, Qianna
Wang, Huijie
Shi, Hongwei
Feng, Ruiyun
Genome-Wide Identification and Characterization of the Potato IQD Family During Development and Stress
title Genome-Wide Identification and Characterization of the Potato IQD Family During Development and Stress
title_full Genome-Wide Identification and Characterization of the Potato IQD Family During Development and Stress
title_fullStr Genome-Wide Identification and Characterization of the Potato IQD Family During Development and Stress
title_full_unstemmed Genome-Wide Identification and Characterization of the Potato IQD Family During Development and Stress
title_short Genome-Wide Identification and Characterization of the Potato IQD Family During Development and Stress
title_sort genome-wide identification and characterization of the potato iqd family during development and stress
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354571/
https://www.ncbi.nlm.nih.gov/pubmed/34386041
http://dx.doi.org/10.3389/fgene.2021.693936
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