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Quantitative mapping of the cellular small RNA landscape with AQRNA-seq
Current next-generation RNA sequencing methods do not provide accurate quantification of small RNAs within a sample due to sequence-dependent biases in capture, ligation, and amplification during library preparation. We present a method, Absolute Quantification (AQ) RNA-seq, that minimizes biases an...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355021/ https://www.ncbi.nlm.nih.gov/pubmed/33859402 http://dx.doi.org/10.1038/s41587-021-00874-y |