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Quantitative mapping of the cellular small RNA landscape with AQRNA-seq

Current next-generation RNA sequencing methods do not provide accurate quantification of small RNAs within a sample due to sequence-dependent biases in capture, ligation, and amplification during library preparation. We present a method, Absolute Quantification (AQ) RNA-seq, that minimizes biases an...

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Detalles Bibliográficos
Autores principales: Hu, Jennifer F., Yim, Daniel, Ma, Duanduan, Huber, Sabrina M., Davis, Nick, Bacusmo, Jo Marie, Vermeulen, Sidney, Zhou, Jieliang, Begley, Thomas J., DeMott, Michael S., Levine, Stuart S., de Crécy-Lagard, Valerie, Dedon, Peter C., Cao, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355021/
https://www.ncbi.nlm.nih.gov/pubmed/33859402
http://dx.doi.org/10.1038/s41587-021-00874-y