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Clinical validation of optimised RT-LAMP for the diagnosis of SARS-CoV-2 infection

We have optimised a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of SARS-CoV-2 from extracted RNA for clinical application. We improved the stability and reliability of the RT-LAMP assay by the addition of a temperature-dependent switch oligonucleoti...

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Detalles Bibliográficos
Autores principales: Lim, Boon, Ratcliff, Jeremy, Nawrot, Dorota A., Yu, Yejiong, Sanghani, Harshmeena R., Hsu, Chia-Chen, Peto, Leon, Evans, Simon, Hodgson, Susanne H., Skeva, Aikaterini, Adam, Maria, Panopoulou, Maria, Zois, Christos E., Poncin, Katy, Vasudevan, Sridhar R., Dai, Siqi, Ren, Shuai, Chang, Hong, Cui, Zhanfeng, Simmonds, Peter, Huang, Wei E., Andersson, Monique I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355225/
https://www.ncbi.nlm.nih.gov/pubmed/34376716
http://dx.doi.org/10.1038/s41598-021-95607-1

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