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Clinical validation of optimised RT-LAMP for the diagnosis of SARS-CoV-2 infection
We have optimised a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of SARS-CoV-2 from extracted RNA for clinical application. We improved the stability and reliability of the RT-LAMP assay by the addition of a temperature-dependent switch oligonucleoti...
Autores principales: | Lim, Boon, Ratcliff, Jeremy, Nawrot, Dorota A., Yu, Yejiong, Sanghani, Harshmeena R., Hsu, Chia-Chen, Peto, Leon, Evans, Simon, Hodgson, Susanne H., Skeva, Aikaterini, Adam, Maria, Panopoulou, Maria, Zois, Christos E., Poncin, Katy, Vasudevan, Sridhar R., Dai, Siqi, Ren, Shuai, Chang, Hong, Cui, Zhanfeng, Simmonds, Peter, Huang, Wei E., Andersson, Monique I. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355225/ https://www.ncbi.nlm.nih.gov/pubmed/34376716 http://dx.doi.org/10.1038/s41598-021-95607-1 |
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