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Natural deep eutectic systems, an emerging class of cryoprotectant agents

This work aimed at evaluating the potential of using natural deep eutectic systems (NADES) as cryoprotectant agents (CPAs). Several combinations between natural primary metabolites that have been identified in animals that live in extreme cold climates were prepared. All systems showed very little c...

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Detalles Bibliográficos
Autores principales: Jesus, Ana Rita, Meneses, Liane, Duarte, Ana Rita C., Paiva, Alexandre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355309/
https://www.ncbi.nlm.nih.gov/pubmed/33974836
http://dx.doi.org/10.1016/j.cryobiol.2021.05.002
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author Jesus, Ana Rita
Meneses, Liane
Duarte, Ana Rita C.
Paiva, Alexandre
author_facet Jesus, Ana Rita
Meneses, Liane
Duarte, Ana Rita C.
Paiva, Alexandre
author_sort Jesus, Ana Rita
collection PubMed
description This work aimed at evaluating the potential of using natural deep eutectic systems (NADES) as cryoprotectant agents (CPAs). Several combinations between natural primary metabolites that have been identified in animals that live in extreme cold climates were prepared. All systems showed very little cytoxicity towards L929 cells at concentrations high as 1–2 M. Moreover, this cell line was highly tolerant to 10% (w/v) of NADES when compared to Me(2)SO. To test NADES as CPAs, two cell lines were used, L929 and HacaT cells. After freeze/thawing cycle, it was possible to observe that for L929 cells, NADES presented similar behaviour to Me(2)SO. For Hacat cell line a significant improvement on post-thawing recovery was observed. Moreover, the results presented herein showed that NADES do not need to be removed from the freezing media after thawing the cells, which is a great advantage of these materials. Additionally, we have shown that NADES can act as CPA when cells are frozen at −20 °C. In overall, the results demonstrate the high potential of NADES to be used in cryobiology as alternative CPAs.
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spelling pubmed-83553092021-08-15 Natural deep eutectic systems, an emerging class of cryoprotectant agents Jesus, Ana Rita Meneses, Liane Duarte, Ana Rita C. Paiva, Alexandre Cryobiology Article This work aimed at evaluating the potential of using natural deep eutectic systems (NADES) as cryoprotectant agents (CPAs). Several combinations between natural primary metabolites that have been identified in animals that live in extreme cold climates were prepared. All systems showed very little cytoxicity towards L929 cells at concentrations high as 1–2 M. Moreover, this cell line was highly tolerant to 10% (w/v) of NADES when compared to Me(2)SO. To test NADES as CPAs, two cell lines were used, L929 and HacaT cells. After freeze/thawing cycle, it was possible to observe that for L929 cells, NADES presented similar behaviour to Me(2)SO. For Hacat cell line a significant improvement on post-thawing recovery was observed. Moreover, the results presented herein showed that NADES do not need to be removed from the freezing media after thawing the cells, which is a great advantage of these materials. Additionally, we have shown that NADES can act as CPA when cells are frozen at −20 °C. In overall, the results demonstrate the high potential of NADES to be used in cryobiology as alternative CPAs. Elsevier 2021-08 /pmc/articles/PMC8355309/ /pubmed/33974836 http://dx.doi.org/10.1016/j.cryobiol.2021.05.002 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Jesus, Ana Rita
Meneses, Liane
Duarte, Ana Rita C.
Paiva, Alexandre
Natural deep eutectic systems, an emerging class of cryoprotectant agents
title Natural deep eutectic systems, an emerging class of cryoprotectant agents
title_full Natural deep eutectic systems, an emerging class of cryoprotectant agents
title_fullStr Natural deep eutectic systems, an emerging class of cryoprotectant agents
title_full_unstemmed Natural deep eutectic systems, an emerging class of cryoprotectant agents
title_short Natural deep eutectic systems, an emerging class of cryoprotectant agents
title_sort natural deep eutectic systems, an emerging class of cryoprotectant agents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355309/
https://www.ncbi.nlm.nih.gov/pubmed/33974836
http://dx.doi.org/10.1016/j.cryobiol.2021.05.002
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