Cargando…
Natural deep eutectic systems, an emerging class of cryoprotectant agents
This work aimed at evaluating the potential of using natural deep eutectic systems (NADES) as cryoprotectant agents (CPAs). Several combinations between natural primary metabolites that have been identified in animals that live in extreme cold climates were prepared. All systems showed very little c...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355309/ https://www.ncbi.nlm.nih.gov/pubmed/33974836 http://dx.doi.org/10.1016/j.cryobiol.2021.05.002 |
_version_ | 1783736734689787904 |
---|---|
author | Jesus, Ana Rita Meneses, Liane Duarte, Ana Rita C. Paiva, Alexandre |
author_facet | Jesus, Ana Rita Meneses, Liane Duarte, Ana Rita C. Paiva, Alexandre |
author_sort | Jesus, Ana Rita |
collection | PubMed |
description | This work aimed at evaluating the potential of using natural deep eutectic systems (NADES) as cryoprotectant agents (CPAs). Several combinations between natural primary metabolites that have been identified in animals that live in extreme cold climates were prepared. All systems showed very little cytoxicity towards L929 cells at concentrations high as 1–2 M. Moreover, this cell line was highly tolerant to 10% (w/v) of NADES when compared to Me(2)SO. To test NADES as CPAs, two cell lines were used, L929 and HacaT cells. After freeze/thawing cycle, it was possible to observe that for L929 cells, NADES presented similar behaviour to Me(2)SO. For Hacat cell line a significant improvement on post-thawing recovery was observed. Moreover, the results presented herein showed that NADES do not need to be removed from the freezing media after thawing the cells, which is a great advantage of these materials. Additionally, we have shown that NADES can act as CPA when cells are frozen at −20 °C. In overall, the results demonstrate the high potential of NADES to be used in cryobiology as alternative CPAs. |
format | Online Article Text |
id | pubmed-8355309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-83553092021-08-15 Natural deep eutectic systems, an emerging class of cryoprotectant agents Jesus, Ana Rita Meneses, Liane Duarte, Ana Rita C. Paiva, Alexandre Cryobiology Article This work aimed at evaluating the potential of using natural deep eutectic systems (NADES) as cryoprotectant agents (CPAs). Several combinations between natural primary metabolites that have been identified in animals that live in extreme cold climates were prepared. All systems showed very little cytoxicity towards L929 cells at concentrations high as 1–2 M. Moreover, this cell line was highly tolerant to 10% (w/v) of NADES when compared to Me(2)SO. To test NADES as CPAs, two cell lines were used, L929 and HacaT cells. After freeze/thawing cycle, it was possible to observe that for L929 cells, NADES presented similar behaviour to Me(2)SO. For Hacat cell line a significant improvement on post-thawing recovery was observed. Moreover, the results presented herein showed that NADES do not need to be removed from the freezing media after thawing the cells, which is a great advantage of these materials. Additionally, we have shown that NADES can act as CPA when cells are frozen at −20 °C. In overall, the results demonstrate the high potential of NADES to be used in cryobiology as alternative CPAs. Elsevier 2021-08 /pmc/articles/PMC8355309/ /pubmed/33974836 http://dx.doi.org/10.1016/j.cryobiol.2021.05.002 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Jesus, Ana Rita Meneses, Liane Duarte, Ana Rita C. Paiva, Alexandre Natural deep eutectic systems, an emerging class of cryoprotectant agents |
title | Natural deep eutectic systems, an emerging class of cryoprotectant agents |
title_full | Natural deep eutectic systems, an emerging class of cryoprotectant agents |
title_fullStr | Natural deep eutectic systems, an emerging class of cryoprotectant agents |
title_full_unstemmed | Natural deep eutectic systems, an emerging class of cryoprotectant agents |
title_short | Natural deep eutectic systems, an emerging class of cryoprotectant agents |
title_sort | natural deep eutectic systems, an emerging class of cryoprotectant agents |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355309/ https://www.ncbi.nlm.nih.gov/pubmed/33974836 http://dx.doi.org/10.1016/j.cryobiol.2021.05.002 |
work_keys_str_mv | AT jesusanarita naturaldeepeutecticsystemsanemergingclassofcryoprotectantagents AT menesesliane naturaldeepeutecticsystemsanemergingclassofcryoprotectantagents AT duarteanaritac naturaldeepeutecticsystemsanemergingclassofcryoprotectantagents AT paivaalexandre naturaldeepeutecticsystemsanemergingclassofcryoprotectantagents |