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Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing
Hydrogel wound dressings can play critical roles in wound healing protecting the wound from trauma or contamination and providing an ideal environment to support the growth of endogenous cells and promote wound closure. This work presents a self-assembling hydrogel dressing that can assist the wound...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355605/ https://www.ncbi.nlm.nih.gov/pubmed/34386265 http://dx.doi.org/10.1093/rb/rbab040 |
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author | Ajovalasit, Alessia Redondo-Gómez, Carlos Sabatino, Maria Antonietta Okesola, Babatunde O Braun, Kristin Mata, Alvaro Dispenza, Clelia |
author_facet | Ajovalasit, Alessia Redondo-Gómez, Carlos Sabatino, Maria Antonietta Okesola, Babatunde O Braun, Kristin Mata, Alvaro Dispenza, Clelia |
author_sort | Ajovalasit, Alessia |
collection | PubMed |
description | Hydrogel wound dressings can play critical roles in wound healing protecting the wound from trauma or contamination and providing an ideal environment to support the growth of endogenous cells and promote wound closure. This work presents a self-assembling hydrogel dressing that can assist the wound repair process mimicking the hierarchical structure of skin extracellular matrix. To this aim, the co-assembly behaviour of a carboxylated variant of xyloglucan (CXG) with a peptide amphiphile (PA-H3) has been investigated to generate hierarchical constructs with tuneable molecular composition, structure, and properties. Transmission electron microscopy and circular dichroism at a low concentration shows that CXG and PA-H3 co-assemble into nanofibres by hydrophobic and electrostatic interactions and further aggregate into nanofibre bundles and networks. At a higher concentration, CXG and PA-H3 yield hydrogels that have been characterized for their morphology by scanning electron microscopy and for the mechanical properties by small-amplitude oscillatory shear rheological measurements and compression tests at different CXG/PA-H3 ratios. A preliminary biological evaluation has been carried out both in vitro with HaCat cells and in vivo in a mouse model. |
format | Online Article Text |
id | pubmed-8355605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-83556052021-08-11 Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing Ajovalasit, Alessia Redondo-Gómez, Carlos Sabatino, Maria Antonietta Okesola, Babatunde O Braun, Kristin Mata, Alvaro Dispenza, Clelia Regen Biomater Research Article Hydrogel wound dressings can play critical roles in wound healing protecting the wound from trauma or contamination and providing an ideal environment to support the growth of endogenous cells and promote wound closure. This work presents a self-assembling hydrogel dressing that can assist the wound repair process mimicking the hierarchical structure of skin extracellular matrix. To this aim, the co-assembly behaviour of a carboxylated variant of xyloglucan (CXG) with a peptide amphiphile (PA-H3) has been investigated to generate hierarchical constructs with tuneable molecular composition, structure, and properties. Transmission electron microscopy and circular dichroism at a low concentration shows that CXG and PA-H3 co-assemble into nanofibres by hydrophobic and electrostatic interactions and further aggregate into nanofibre bundles and networks. At a higher concentration, CXG and PA-H3 yield hydrogels that have been characterized for their morphology by scanning electron microscopy and for the mechanical properties by small-amplitude oscillatory shear rheological measurements and compression tests at different CXG/PA-H3 ratios. A preliminary biological evaluation has been carried out both in vitro with HaCat cells and in vivo in a mouse model. Oxford University Press 2021-08-11 /pmc/articles/PMC8355605/ /pubmed/34386265 http://dx.doi.org/10.1093/rb/rbab040 Text en © The Author(s) 2021. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ajovalasit, Alessia Redondo-Gómez, Carlos Sabatino, Maria Antonietta Okesola, Babatunde O Braun, Kristin Mata, Alvaro Dispenza, Clelia Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing |
title | Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing |
title_full | Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing |
title_fullStr | Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing |
title_full_unstemmed | Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing |
title_short | Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing |
title_sort | carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355605/ https://www.ncbi.nlm.nih.gov/pubmed/34386265 http://dx.doi.org/10.1093/rb/rbab040 |
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