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Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing

Hydrogel wound dressings can play critical roles in wound healing protecting the wound from trauma or contamination and providing an ideal environment to support the growth of endogenous cells and promote wound closure. This work presents a self-assembling hydrogel dressing that can assist the wound...

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Autores principales: Ajovalasit, Alessia, Redondo-Gómez, Carlos, Sabatino, Maria Antonietta, Okesola, Babatunde O, Braun, Kristin, Mata, Alvaro, Dispenza, Clelia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355605/
https://www.ncbi.nlm.nih.gov/pubmed/34386265
http://dx.doi.org/10.1093/rb/rbab040
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author Ajovalasit, Alessia
Redondo-Gómez, Carlos
Sabatino, Maria Antonietta
Okesola, Babatunde O
Braun, Kristin
Mata, Alvaro
Dispenza, Clelia
author_facet Ajovalasit, Alessia
Redondo-Gómez, Carlos
Sabatino, Maria Antonietta
Okesola, Babatunde O
Braun, Kristin
Mata, Alvaro
Dispenza, Clelia
author_sort Ajovalasit, Alessia
collection PubMed
description Hydrogel wound dressings can play critical roles in wound healing protecting the wound from trauma or contamination and providing an ideal environment to support the growth of endogenous cells and promote wound closure. This work presents a self-assembling hydrogel dressing that can assist the wound repair process mimicking the hierarchical structure of skin extracellular matrix. To this aim, the co-assembly behaviour of a carboxylated variant of xyloglucan (CXG) with a peptide amphiphile (PA-H3) has been investigated to generate hierarchical constructs with tuneable molecular composition, structure, and properties. Transmission electron microscopy and circular dichroism at a low concentration shows that CXG and PA-H3 co-assemble into nanofibres by hydrophobic and electrostatic interactions and further aggregate into nanofibre bundles and networks. At a higher concentration, CXG and PA-H3 yield hydrogels that have been characterized for their morphology by scanning electron microscopy and for the mechanical properties by small-amplitude oscillatory shear rheological measurements and compression tests at different CXG/PA-H3 ratios. A preliminary biological evaluation has been carried out both in vitro with HaCat cells and in vivo in a mouse model.
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spelling pubmed-83556052021-08-11 Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing Ajovalasit, Alessia Redondo-Gómez, Carlos Sabatino, Maria Antonietta Okesola, Babatunde O Braun, Kristin Mata, Alvaro Dispenza, Clelia Regen Biomater Research Article Hydrogel wound dressings can play critical roles in wound healing protecting the wound from trauma or contamination and providing an ideal environment to support the growth of endogenous cells and promote wound closure. This work presents a self-assembling hydrogel dressing that can assist the wound repair process mimicking the hierarchical structure of skin extracellular matrix. To this aim, the co-assembly behaviour of a carboxylated variant of xyloglucan (CXG) with a peptide amphiphile (PA-H3) has been investigated to generate hierarchical constructs with tuneable molecular composition, structure, and properties. Transmission electron microscopy and circular dichroism at a low concentration shows that CXG and PA-H3 co-assemble into nanofibres by hydrophobic and electrostatic interactions and further aggregate into nanofibre bundles and networks. At a higher concentration, CXG and PA-H3 yield hydrogels that have been characterized for their morphology by scanning electron microscopy and for the mechanical properties by small-amplitude oscillatory shear rheological measurements and compression tests at different CXG/PA-H3 ratios. A preliminary biological evaluation has been carried out both in vitro with HaCat cells and in vivo in a mouse model. Oxford University Press 2021-08-11 /pmc/articles/PMC8355605/ /pubmed/34386265 http://dx.doi.org/10.1093/rb/rbab040 Text en © The Author(s) 2021. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ajovalasit, Alessia
Redondo-Gómez, Carlos
Sabatino, Maria Antonietta
Okesola, Babatunde O
Braun, Kristin
Mata, Alvaro
Dispenza, Clelia
Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing
title Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing
title_full Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing
title_fullStr Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing
title_full_unstemmed Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing
title_short Carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing
title_sort carboxylated-xyloglucan and peptide amphiphile co-assembly in wound healing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355605/
https://www.ncbi.nlm.nih.gov/pubmed/34386265
http://dx.doi.org/10.1093/rb/rbab040
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