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Instant in-situ Tissue Repair by Biodegradable PLA/Gelatin Nanofibrous Membrane Using a 3D Printed Handheld Electrospinning Device

Background: This study aims to design a 3D printed handheld electrospinning device and evaluate its effect on the rapid repair of mouse skin wounds. Methods: The device was developed by Solidworks and printed by Object 350 photosensitive resin printer. The polylactic acid (PLA)/gelatin blend was use...

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Autores principales: Chen, Hongrang, Zhang, Haitao, Shen, Yun, Dai, Xingliang, Wang, Xuanzhi, Deng, Kunxue, Long, Xiaoyan, Liu, Libiao, Zhang, Xinzhi, Li, Yongsheng, Xu, Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355707/
https://www.ncbi.nlm.nih.gov/pubmed/34395397
http://dx.doi.org/10.3389/fbioe.2021.684105
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author Chen, Hongrang
Zhang, Haitao
Shen, Yun
Dai, Xingliang
Wang, Xuanzhi
Deng, Kunxue
Long, Xiaoyan
Liu, Libiao
Zhang, Xinzhi
Li, Yongsheng
Xu, Tao
author_facet Chen, Hongrang
Zhang, Haitao
Shen, Yun
Dai, Xingliang
Wang, Xuanzhi
Deng, Kunxue
Long, Xiaoyan
Liu, Libiao
Zhang, Xinzhi
Li, Yongsheng
Xu, Tao
author_sort Chen, Hongrang
collection PubMed
description Background: This study aims to design a 3D printed handheld electrospinning device and evaluate its effect on the rapid repair of mouse skin wounds. Methods: The device was developed by Solidworks and printed by Object 350 photosensitive resin printer. The polylactic acid (PLA)/gelatin blend was used as the raw material to fabricate in-situ degradable nanofiber scaffolds. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and water vapor permeability test were used to evaluate the material properties of the scaffolds; cytotoxicity test was performed to evaluate material/residual solvent toxicity, and in situ tissue repair experiments in Balb/c mouse were performed. Results: The 3D printed handheld electrospinning device successfully fabricates PLA/gelatin nanofibrous membrane with uniformly layered nanofibers and good biocompatibility. Animal experiments showed that the mice in the experimental group had complete skin repair. Conclusions: The 3D printed handheld device can achieve in situ repair of full-thickness defects in mouse skin.
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spelling pubmed-83557072021-08-12 Instant in-situ Tissue Repair by Biodegradable PLA/Gelatin Nanofibrous Membrane Using a 3D Printed Handheld Electrospinning Device Chen, Hongrang Zhang, Haitao Shen, Yun Dai, Xingliang Wang, Xuanzhi Deng, Kunxue Long, Xiaoyan Liu, Libiao Zhang, Xinzhi Li, Yongsheng Xu, Tao Front Bioeng Biotechnol Bioengineering and Biotechnology Background: This study aims to design a 3D printed handheld electrospinning device and evaluate its effect on the rapid repair of mouse skin wounds. Methods: The device was developed by Solidworks and printed by Object 350 photosensitive resin printer. The polylactic acid (PLA)/gelatin blend was used as the raw material to fabricate in-situ degradable nanofiber scaffolds. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and water vapor permeability test were used to evaluate the material properties of the scaffolds; cytotoxicity test was performed to evaluate material/residual solvent toxicity, and in situ tissue repair experiments in Balb/c mouse were performed. Results: The 3D printed handheld electrospinning device successfully fabricates PLA/gelatin nanofibrous membrane with uniformly layered nanofibers and good biocompatibility. Animal experiments showed that the mice in the experimental group had complete skin repair. Conclusions: The 3D printed handheld device can achieve in situ repair of full-thickness defects in mouse skin. Frontiers Media S.A. 2021-07-28 /pmc/articles/PMC8355707/ /pubmed/34395397 http://dx.doi.org/10.3389/fbioe.2021.684105 Text en Copyright © 2021 Chen, Zhang, Shen, Dai, Wang, Deng, Long, Liu, Zhang, Li and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Chen, Hongrang
Zhang, Haitao
Shen, Yun
Dai, Xingliang
Wang, Xuanzhi
Deng, Kunxue
Long, Xiaoyan
Liu, Libiao
Zhang, Xinzhi
Li, Yongsheng
Xu, Tao
Instant in-situ Tissue Repair by Biodegradable PLA/Gelatin Nanofibrous Membrane Using a 3D Printed Handheld Electrospinning Device
title Instant in-situ Tissue Repair by Biodegradable PLA/Gelatin Nanofibrous Membrane Using a 3D Printed Handheld Electrospinning Device
title_full Instant in-situ Tissue Repair by Biodegradable PLA/Gelatin Nanofibrous Membrane Using a 3D Printed Handheld Electrospinning Device
title_fullStr Instant in-situ Tissue Repair by Biodegradable PLA/Gelatin Nanofibrous Membrane Using a 3D Printed Handheld Electrospinning Device
title_full_unstemmed Instant in-situ Tissue Repair by Biodegradable PLA/Gelatin Nanofibrous Membrane Using a 3D Printed Handheld Electrospinning Device
title_short Instant in-situ Tissue Repair by Biodegradable PLA/Gelatin Nanofibrous Membrane Using a 3D Printed Handheld Electrospinning Device
title_sort instant in-situ tissue repair by biodegradable pla/gelatin nanofibrous membrane using a 3d printed handheld electrospinning device
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355707/
https://www.ncbi.nlm.nih.gov/pubmed/34395397
http://dx.doi.org/10.3389/fbioe.2021.684105
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