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Virus-encoded histone doublets are essential and form nucleosome-like structures

The organization of genomic DNA into defined nucleosomes has long been viewed as a hallmark of eukaryotes. This paradigm has been challenged by the identification of “minimalist” histones in archaea and more recently by the discovery of genes that encode fused remote homologs of the four eukaryotic...

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Autores principales: Liu, Yang, Bisio, Hugo, Toner, Chelsea Marie, Jeudy, Sandra, Philippe, Nadege, Zhou, Keda, Bowerman, Samuel, White, Alison, Edwards, Garrett, Abergel, Chantal, Luger, Karolin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357426/
https://www.ncbi.nlm.nih.gov/pubmed/34297924
http://dx.doi.org/10.1016/j.cell.2021.06.032
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author Liu, Yang
Bisio, Hugo
Toner, Chelsea Marie
Jeudy, Sandra
Philippe, Nadege
Zhou, Keda
Bowerman, Samuel
White, Alison
Edwards, Garrett
Abergel, Chantal
Luger, Karolin
author_facet Liu, Yang
Bisio, Hugo
Toner, Chelsea Marie
Jeudy, Sandra
Philippe, Nadege
Zhou, Keda
Bowerman, Samuel
White, Alison
Edwards, Garrett
Abergel, Chantal
Luger, Karolin
author_sort Liu, Yang
collection PubMed
description The organization of genomic DNA into defined nucleosomes has long been viewed as a hallmark of eukaryotes. This paradigm has been challenged by the identification of “minimalist” histones in archaea and more recently by the discovery of genes that encode fused remote homologs of the four eukaryotic histones in Marseilleviridae, a subfamily of giant viruses that infect amoebae. We demonstrate that viral doublet histones are essential for viral infectivity, localize to cytoplasmic viral factories after virus infection, and ultimately are found in the mature virions. Cryogenic electron microscopy (cryo-EM) structures of viral nucleosome-like particles show strong similarities to eukaryotic nucleosomes despite the limited sequence identify. The unique connectors that link the histone chains contribute to the observed instability of viral nucleosomes, and some histone tails assume structural roles. Our results further expand the range of “organisms” that require nucleosomes and suggest a specialized function of histones in the biology of these unusual viruses.
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spelling pubmed-83574262021-08-12 Virus-encoded histone doublets are essential and form nucleosome-like structures Liu, Yang Bisio, Hugo Toner, Chelsea Marie Jeudy, Sandra Philippe, Nadege Zhou, Keda Bowerman, Samuel White, Alison Edwards, Garrett Abergel, Chantal Luger, Karolin Cell Article The organization of genomic DNA into defined nucleosomes has long been viewed as a hallmark of eukaryotes. This paradigm has been challenged by the identification of “minimalist” histones in archaea and more recently by the discovery of genes that encode fused remote homologs of the four eukaryotic histones in Marseilleviridae, a subfamily of giant viruses that infect amoebae. We demonstrate that viral doublet histones are essential for viral infectivity, localize to cytoplasmic viral factories after virus infection, and ultimately are found in the mature virions. Cryogenic electron microscopy (cryo-EM) structures of viral nucleosome-like particles show strong similarities to eukaryotic nucleosomes despite the limited sequence identify. The unique connectors that link the histone chains contribute to the observed instability of viral nucleosomes, and some histone tails assume structural roles. Our results further expand the range of “organisms” that require nucleosomes and suggest a specialized function of histones in the biology of these unusual viruses. Cell Press 2021-08-05 /pmc/articles/PMC8357426/ /pubmed/34297924 http://dx.doi.org/10.1016/j.cell.2021.06.032 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Liu, Yang
Bisio, Hugo
Toner, Chelsea Marie
Jeudy, Sandra
Philippe, Nadege
Zhou, Keda
Bowerman, Samuel
White, Alison
Edwards, Garrett
Abergel, Chantal
Luger, Karolin
Virus-encoded histone doublets are essential and form nucleosome-like structures
title Virus-encoded histone doublets are essential and form nucleosome-like structures
title_full Virus-encoded histone doublets are essential and form nucleosome-like structures
title_fullStr Virus-encoded histone doublets are essential and form nucleosome-like structures
title_full_unstemmed Virus-encoded histone doublets are essential and form nucleosome-like structures
title_short Virus-encoded histone doublets are essential and form nucleosome-like structures
title_sort virus-encoded histone doublets are essential and form nucleosome-like structures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357426/
https://www.ncbi.nlm.nih.gov/pubmed/34297924
http://dx.doi.org/10.1016/j.cell.2021.06.032
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