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Virus-encoded histone doublets are essential and form nucleosome-like structures
The organization of genomic DNA into defined nucleosomes has long been viewed as a hallmark of eukaryotes. This paradigm has been challenged by the identification of “minimalist” histones in archaea and more recently by the discovery of genes that encode fused remote homologs of the four eukaryotic...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357426/ https://www.ncbi.nlm.nih.gov/pubmed/34297924 http://dx.doi.org/10.1016/j.cell.2021.06.032 |
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author | Liu, Yang Bisio, Hugo Toner, Chelsea Marie Jeudy, Sandra Philippe, Nadege Zhou, Keda Bowerman, Samuel White, Alison Edwards, Garrett Abergel, Chantal Luger, Karolin |
author_facet | Liu, Yang Bisio, Hugo Toner, Chelsea Marie Jeudy, Sandra Philippe, Nadege Zhou, Keda Bowerman, Samuel White, Alison Edwards, Garrett Abergel, Chantal Luger, Karolin |
author_sort | Liu, Yang |
collection | PubMed |
description | The organization of genomic DNA into defined nucleosomes has long been viewed as a hallmark of eukaryotes. This paradigm has been challenged by the identification of “minimalist” histones in archaea and more recently by the discovery of genes that encode fused remote homologs of the four eukaryotic histones in Marseilleviridae, a subfamily of giant viruses that infect amoebae. We demonstrate that viral doublet histones are essential for viral infectivity, localize to cytoplasmic viral factories after virus infection, and ultimately are found in the mature virions. Cryogenic electron microscopy (cryo-EM) structures of viral nucleosome-like particles show strong similarities to eukaryotic nucleosomes despite the limited sequence identify. The unique connectors that link the histone chains contribute to the observed instability of viral nucleosomes, and some histone tails assume structural roles. Our results further expand the range of “organisms” that require nucleosomes and suggest a specialized function of histones in the biology of these unusual viruses. |
format | Online Article Text |
id | pubmed-8357426 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-83574262021-08-12 Virus-encoded histone doublets are essential and form nucleosome-like structures Liu, Yang Bisio, Hugo Toner, Chelsea Marie Jeudy, Sandra Philippe, Nadege Zhou, Keda Bowerman, Samuel White, Alison Edwards, Garrett Abergel, Chantal Luger, Karolin Cell Article The organization of genomic DNA into defined nucleosomes has long been viewed as a hallmark of eukaryotes. This paradigm has been challenged by the identification of “minimalist” histones in archaea and more recently by the discovery of genes that encode fused remote homologs of the four eukaryotic histones in Marseilleviridae, a subfamily of giant viruses that infect amoebae. We demonstrate that viral doublet histones are essential for viral infectivity, localize to cytoplasmic viral factories after virus infection, and ultimately are found in the mature virions. Cryogenic electron microscopy (cryo-EM) structures of viral nucleosome-like particles show strong similarities to eukaryotic nucleosomes despite the limited sequence identify. The unique connectors that link the histone chains contribute to the observed instability of viral nucleosomes, and some histone tails assume structural roles. Our results further expand the range of “organisms” that require nucleosomes and suggest a specialized function of histones in the biology of these unusual viruses. Cell Press 2021-08-05 /pmc/articles/PMC8357426/ /pubmed/34297924 http://dx.doi.org/10.1016/j.cell.2021.06.032 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Liu, Yang Bisio, Hugo Toner, Chelsea Marie Jeudy, Sandra Philippe, Nadege Zhou, Keda Bowerman, Samuel White, Alison Edwards, Garrett Abergel, Chantal Luger, Karolin Virus-encoded histone doublets are essential and form nucleosome-like structures |
title | Virus-encoded histone doublets are essential and form nucleosome-like structures |
title_full | Virus-encoded histone doublets are essential and form nucleosome-like structures |
title_fullStr | Virus-encoded histone doublets are essential and form nucleosome-like structures |
title_full_unstemmed | Virus-encoded histone doublets are essential and form nucleosome-like structures |
title_short | Virus-encoded histone doublets are essential and form nucleosome-like structures |
title_sort | virus-encoded histone doublets are essential and form nucleosome-like structures |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357426/ https://www.ncbi.nlm.nih.gov/pubmed/34297924 http://dx.doi.org/10.1016/j.cell.2021.06.032 |
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