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A Genome-Wide Analysis of Antibiotic Producing Genes in Streptomyces globisporus SP6C4
Soil is the major source of plant-associated microbes. Several fungal and bacterial species live within plant tissues. Actinomycetes are well known for producing a variety of antibiotics, and they contribute to improving plant health. In our previous report, Streptomyces globisporus SP6C4 colonized...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Plant Pathology
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357572/ https://www.ncbi.nlm.nih.gov/pubmed/34365750 http://dx.doi.org/10.5423/PPJ.NT.03.2021.0047 |
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author | Kim, Da-Ran Kwak, Youn-Sig |
author_facet | Kim, Da-Ran Kwak, Youn-Sig |
author_sort | Kim, Da-Ran |
collection | PubMed |
description | Soil is the major source of plant-associated microbes. Several fungal and bacterial species live within plant tissues. Actinomycetes are well known for producing a variety of antibiotics, and they contribute to improving plant health. In our previous report, Streptomyces globisporus SP6C4 colonized plant tissues and was able to move to other tissues from the initially colonized ones. This strain has excellent antifungal and antibacterial activities and provides a suppressive effect upon various plant diseases. Here, we report the genome-wide analysis of antibiotic producing genes in S. globisporus SP6C4. A total of 15 secondary metabolite biosynthetic gene clusters were predicted using antiSMASH. We used the CRISPR/Cas9 mutagenesis system, and each biosynthetic gene was predicted via protein basic local alignment search tool (BLAST) and rapid annotation using subsystems technology (RAST) server. Three gene clusters were shown to exhibit antifungal or antibacterial activity, viz. cluster 16 (lasso peptide), cluster 17 (thiopeptide-lantipeptide), and cluster 20 (lantipeptide). The results of the current study showed that SP6C4 has a variety of antimicrobial activities, and this strain is beneficial in agriculture. |
format | Online Article Text |
id | pubmed-8357572 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Korean Society of Plant Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-83575722021-08-13 A Genome-Wide Analysis of Antibiotic Producing Genes in Streptomyces globisporus SP6C4 Kim, Da-Ran Kwak, Youn-Sig Plant Pathol J Note Soil is the major source of plant-associated microbes. Several fungal and bacterial species live within plant tissues. Actinomycetes are well known for producing a variety of antibiotics, and they contribute to improving plant health. In our previous report, Streptomyces globisporus SP6C4 colonized plant tissues and was able to move to other tissues from the initially colonized ones. This strain has excellent antifungal and antibacterial activities and provides a suppressive effect upon various plant diseases. Here, we report the genome-wide analysis of antibiotic producing genes in S. globisporus SP6C4. A total of 15 secondary metabolite biosynthetic gene clusters were predicted using antiSMASH. We used the CRISPR/Cas9 mutagenesis system, and each biosynthetic gene was predicted via protein basic local alignment search tool (BLAST) and rapid annotation using subsystems technology (RAST) server. Three gene clusters were shown to exhibit antifungal or antibacterial activity, viz. cluster 16 (lasso peptide), cluster 17 (thiopeptide-lantipeptide), and cluster 20 (lantipeptide). The results of the current study showed that SP6C4 has a variety of antimicrobial activities, and this strain is beneficial in agriculture. Korean Society of Plant Pathology 2021-08 2021-08-01 /pmc/articles/PMC8357572/ /pubmed/34365750 http://dx.doi.org/10.5423/PPJ.NT.03.2021.0047 Text en © The Korean Society of Plant Pathology https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Note Kim, Da-Ran Kwak, Youn-Sig A Genome-Wide Analysis of Antibiotic Producing Genes in Streptomyces globisporus SP6C4 |
title | A Genome-Wide Analysis of Antibiotic Producing Genes in Streptomyces globisporus SP6C4 |
title_full | A Genome-Wide Analysis of Antibiotic Producing Genes in Streptomyces globisporus SP6C4 |
title_fullStr | A Genome-Wide Analysis of Antibiotic Producing Genes in Streptomyces globisporus SP6C4 |
title_full_unstemmed | A Genome-Wide Analysis of Antibiotic Producing Genes in Streptomyces globisporus SP6C4 |
title_short | A Genome-Wide Analysis of Antibiotic Producing Genes in Streptomyces globisporus SP6C4 |
title_sort | genome-wide analysis of antibiotic producing genes in streptomyces globisporus sp6c4 |
topic | Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357572/ https://www.ncbi.nlm.nih.gov/pubmed/34365750 http://dx.doi.org/10.5423/PPJ.NT.03.2021.0047 |
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