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Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactylamixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae)

Sea cucumber taxonomy and systematics has in the past heavily relied on gross external and internal anatomy, ossicle assemblage in different tissues, and molecular characterisation, with coloration, habitat, and geographical and bathymethric distribution also considered important parameters. In the...

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Autores principales: Samyn, Yves, Sonet, Gontran, d’Acoz, Cédric d’Udekem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357699/
https://www.ncbi.nlm.nih.gov/pubmed/34393568
http://dx.doi.org/10.3897/zookeys.1054.67088
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author Samyn, Yves
Sonet, Gontran
d’Acoz, Cédric d’Udekem
author_facet Samyn, Yves
Sonet, Gontran
d’Acoz, Cédric d’Udekem
author_sort Samyn, Yves
collection PubMed
description Sea cucumber taxonomy and systematics has in the past heavily relied on gross external and internal anatomy, ossicle assemblage in different tissues, and molecular characterisation, with coloration, habitat, and geographical and bathymethric distribution also considered important parameters. In the present paper, we made these observations and techniques in detail and complemented them with the novel technique of micro-computed tomography of the calcareous ring. We investigated a single European species, the so-called gravel sea cucumber, Neopentadactylamixta (Östergren, 1898), using recently collected material from the Chausey Islands, Normandy, France. We redescribed the species, illustrated its ossicle assemblage through scanning electron microscopy, and visualised the calcareous ring through stacking photography and through micro-CT scanning. Additionally, a DNA fragment of 955 base pairs of the 18S ribosomal RNA gene was sequenced from one specimen, which showed a high similarity with the only sequence of N.mixta publicly available. We completed this integrative study by providing a detailed distribution of the occurrence of N.mixta based on published, verifiable accounts.
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spelling pubmed-83576992021-08-13 Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactylamixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae) Samyn, Yves Sonet, Gontran d’Acoz, Cédric d’Udekem Zookeys Research Article Sea cucumber taxonomy and systematics has in the past heavily relied on gross external and internal anatomy, ossicle assemblage in different tissues, and molecular characterisation, with coloration, habitat, and geographical and bathymethric distribution also considered important parameters. In the present paper, we made these observations and techniques in detail and complemented them with the novel technique of micro-computed tomography of the calcareous ring. We investigated a single European species, the so-called gravel sea cucumber, Neopentadactylamixta (Östergren, 1898), using recently collected material from the Chausey Islands, Normandy, France. We redescribed the species, illustrated its ossicle assemblage through scanning electron microscopy, and visualised the calcareous ring through stacking photography and through micro-CT scanning. Additionally, a DNA fragment of 955 base pairs of the 18S ribosomal RNA gene was sequenced from one specimen, which showed a high similarity with the only sequence of N.mixta publicly available. We completed this integrative study by providing a detailed distribution of the occurrence of N.mixta based on published, verifiable accounts. Pensoft Publishers 2021-08-04 /pmc/articles/PMC8357699/ /pubmed/34393568 http://dx.doi.org/10.3897/zookeys.1054.67088 Text en Yves Samyn, Gontran Sonet, Cedric d'Udekem d'Acoz https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Samyn, Yves
Sonet, Gontran
d’Acoz, Cédric d’Udekem
Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactylamixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae)
title Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactylamixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae)
title_full Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactylamixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae)
title_fullStr Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactylamixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae)
title_full_unstemmed Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactylamixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae)
title_short Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactylamixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae)
title_sort exploring the use of micro-computed tomography (micro-ct) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber neopentadactylamixta (östergren, 1898) (echinodermata, holothuroidea, phyllophoridae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357699/
https://www.ncbi.nlm.nih.gov/pubmed/34393568
http://dx.doi.org/10.3897/zookeys.1054.67088
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