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Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine

Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance...

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Detalles Bibliográficos
Autores principales: Zhang, Jian, Fang, Xin, Mao, Yu, Qi, Haochen, Wu, Jayne, Liu, Xiaoru, You, Fangshuo, Zhao, Wenci, Chen, Ying, Zheng, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357935/
https://www.ncbi.nlm.nih.gov/pubmed/34075052
http://dx.doi.org/10.1038/s41538-021-00094-3
Descripción
Sumario:Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10(−6) ng/mL in 20 s, with a wide linear range of 10(−5)–10(−1) ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.