Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine

Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance...

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Autores principales: Zhang, Jian, Fang, Xin, Mao, Yu, Qi, Haochen, Wu, Jayne, Liu, Xiaoru, You, Fangshuo, Zhao, Wenci, Chen, Ying, Zheng, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357935/
https://www.ncbi.nlm.nih.gov/pubmed/34075052
http://dx.doi.org/10.1038/s41538-021-00094-3
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author Zhang, Jian
Fang, Xin
Mao, Yu
Qi, Haochen
Wu, Jayne
Liu, Xiaoru
You, Fangshuo
Zhao, Wenci
Chen, Ying
Zheng, Lei
author_facet Zhang, Jian
Fang, Xin
Mao, Yu
Qi, Haochen
Wu, Jayne
Liu, Xiaoru
You, Fangshuo
Zhao, Wenci
Chen, Ying
Zheng, Lei
author_sort Zhang, Jian
collection PubMed
description Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10(−6) ng/mL in 20 s, with a wide linear range of 10(−5)–10(−1) ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.
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spelling pubmed-83579352021-08-30 Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine Zhang, Jian Fang, Xin Mao, Yu Qi, Haochen Wu, Jayne Liu, Xiaoru You, Fangshuo Zhao, Wenci Chen, Ying Zheng, Lei NPJ Sci Food Article Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10(−6) ng/mL in 20 s, with a wide linear range of 10(−5)–10(−1) ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine. Nature Publishing Group UK 2021-06-01 /pmc/articles/PMC8357935/ /pubmed/34075052 http://dx.doi.org/10.1038/s41538-021-00094-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zhang, Jian
Fang, Xin
Mao, Yu
Qi, Haochen
Wu, Jayne
Liu, Xiaoru
You, Fangshuo
Zhao, Wenci
Chen, Ying
Zheng, Lei
Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
title Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
title_full Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
title_fullStr Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
title_full_unstemmed Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
title_short Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
title_sort real-time, selective, and low-cost detection of trace level sars-cov-2 spike-protein for cold-chain food quarantine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8357935/
https://www.ncbi.nlm.nih.gov/pubmed/34075052
http://dx.doi.org/10.1038/s41538-021-00094-3
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