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Identification of Novel miRNAs in the F8 Gene Via Bioinformatics Tools

BACKGROUND: Hemophilia A is an X-linked bleeding disorder resulting in a deficiency of plasma clotting factor VIII and caused by mutations in the FVIII gene (F8 gene). MicroRNAs (miRNAs) in body fluids are promising biomarker candidates for Hemophilia A, due to their stability in body fluids and acc...

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Detalles Bibliográficos
Autores principales: Rezaei, Halimeh, Motovali-Bashi, Majid, Khalilian, Sheyda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Institute of Genetic Engineering and Biotechnology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358171/
https://www.ncbi.nlm.nih.gov/pubmed/34435059
http://dx.doi.org/10.30498/IJB.2021.2700
Descripción
Sumario:BACKGROUND: Hemophilia A is an X-linked bleeding disorder resulting in a deficiency of plasma clotting factor VIII and caused by mutations in the FVIII gene (F8 gene). MicroRNAs (miRNAs) in body fluids are promising biomarker candidates for Hemophilia A, due to their stability in body fluids and accessibility by non- or minimally-invasive procedures. Therefore; Advances in miRNA analysis methods resulted in a wide range of publications on miRNAs as putative biomarkers. OBJECTIVE: Here we tried to scan the F8 gene region to predict a novel miRNA and identify it as a regulator of the F8 gene. MATERIALS AND METHODS: To this aim, the ability to express novel miRNAs in F8 locus was assessed via reliable bioinformatics databases such as SSCprofiler, RNAfold, miREval, FOMmiR, MaturBayes, miRFIND, UCSC genome browser, Deep Sequencing, and miRBase. RESULTS: Data analysis from the relevant databases offers one stem-loop structure that is predicted to express a novel miRNA CONCLUSIONS: The diagnosis of Hemophilia A with the help of these types of biomarkers is a non-invasive procedure that has been demonstrated to have a significant role in the early diagnosis of the disease. Hopefully, the proposed candidate sequence will be confirmed in vitro and become a non-invasive biomarker in the near future