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Identification of Novel miRNAs in the F8 Gene Via Bioinformatics Tools
BACKGROUND: Hemophilia A is an X-linked bleeding disorder resulting in a deficiency of plasma clotting factor VIII and caused by mutations in the FVIII gene (F8 gene). MicroRNAs (miRNAs) in body fluids are promising biomarker candidates for Hemophilia A, due to their stability in body fluids and acc...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Institute of Genetic Engineering and Biotechnology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358171/ https://www.ncbi.nlm.nih.gov/pubmed/34435059 http://dx.doi.org/10.30498/IJB.2021.2700 |
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author | Rezaei, Halimeh Motovali-Bashi, Majid Khalilian, Sheyda |
author_facet | Rezaei, Halimeh Motovali-Bashi, Majid Khalilian, Sheyda |
author_sort | Rezaei, Halimeh |
collection | PubMed |
description | BACKGROUND: Hemophilia A is an X-linked bleeding disorder resulting in a deficiency of plasma clotting factor VIII and caused by mutations in the FVIII gene (F8 gene). MicroRNAs (miRNAs) in body fluids are promising biomarker candidates for Hemophilia A, due to their stability in body fluids and accessibility by non- or minimally-invasive procedures. Therefore; Advances in miRNA analysis methods resulted in a wide range of publications on miRNAs as putative biomarkers. OBJECTIVE: Here we tried to scan the F8 gene region to predict a novel miRNA and identify it as a regulator of the F8 gene. MATERIALS AND METHODS: To this aim, the ability to express novel miRNAs in F8 locus was assessed via reliable bioinformatics databases such as SSCprofiler, RNAfold, miREval, FOMmiR, MaturBayes, miRFIND, UCSC genome browser, Deep Sequencing, and miRBase. RESULTS: Data analysis from the relevant databases offers one stem-loop structure that is predicted to express a novel miRNA CONCLUSIONS: The diagnosis of Hemophilia A with the help of these types of biomarkers is a non-invasive procedure that has been demonstrated to have a significant role in the early diagnosis of the disease. Hopefully, the proposed candidate sequence will be confirmed in vitro and become a non-invasive biomarker in the near future |
format | Online Article Text |
id | pubmed-8358171 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | National Institute of Genetic Engineering and Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-83581712021-08-24 Identification of Novel miRNAs in the F8 Gene Via Bioinformatics Tools Rezaei, Halimeh Motovali-Bashi, Majid Khalilian, Sheyda Iran J Biotechnol Brief Report BACKGROUND: Hemophilia A is an X-linked bleeding disorder resulting in a deficiency of plasma clotting factor VIII and caused by mutations in the FVIII gene (F8 gene). MicroRNAs (miRNAs) in body fluids are promising biomarker candidates for Hemophilia A, due to their stability in body fluids and accessibility by non- or minimally-invasive procedures. Therefore; Advances in miRNA analysis methods resulted in a wide range of publications on miRNAs as putative biomarkers. OBJECTIVE: Here we tried to scan the F8 gene region to predict a novel miRNA and identify it as a regulator of the F8 gene. MATERIALS AND METHODS: To this aim, the ability to express novel miRNAs in F8 locus was assessed via reliable bioinformatics databases such as SSCprofiler, RNAfold, miREval, FOMmiR, MaturBayes, miRFIND, UCSC genome browser, Deep Sequencing, and miRBase. RESULTS: Data analysis from the relevant databases offers one stem-loop structure that is predicted to express a novel miRNA CONCLUSIONS: The diagnosis of Hemophilia A with the help of these types of biomarkers is a non-invasive procedure that has been demonstrated to have a significant role in the early diagnosis of the disease. Hopefully, the proposed candidate sequence will be confirmed in vitro and become a non-invasive biomarker in the near future National Institute of Genetic Engineering and Biotechnology 2021-04-01 /pmc/articles/PMC8358171/ /pubmed/34435059 http://dx.doi.org/10.30498/IJB.2021.2700 Text en Copyright: © 2021 The Author(s); Published by Iranian Journal of Biotechnology https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 Unported License, ( http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Report Rezaei, Halimeh Motovali-Bashi, Majid Khalilian, Sheyda Identification of Novel miRNAs in the F8 Gene Via Bioinformatics Tools |
title | Identification of Novel miRNAs in the F8 Gene Via Bioinformatics Tools |
title_full | Identification of Novel miRNAs in the F8 Gene Via Bioinformatics Tools |
title_fullStr | Identification of Novel miRNAs in the F8 Gene Via Bioinformatics Tools |
title_full_unstemmed | Identification of Novel miRNAs in the F8 Gene Via Bioinformatics Tools |
title_short | Identification of Novel miRNAs in the F8 Gene Via Bioinformatics Tools |
title_sort | identification of novel mirnas in the f8 gene via bioinformatics tools |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358171/ https://www.ncbi.nlm.nih.gov/pubmed/34435059 http://dx.doi.org/10.30498/IJB.2021.2700 |
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