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Optimizing the Preparation Procedure of Recombinant PSCA, as a Practical Biomarker in Prostate Cancer

BACKGROUND: The unique expression pattern of prostate stem cell antigen (PSCA) in a number of prevalent neoplasms has made the antigen a great target for cancer researches, and many clinical methods have been developed based on the application of this tumor marker. Hence, optimal PSCA laboratory pro...

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Autores principales: Shahrabi Farahani, Mahboube, Saraygord-Afshari, Neda, M Farajollahi, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Institute of Genetic Engineering and Biotechnology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358172/
https://www.ncbi.nlm.nih.gov/pubmed/34435055
http://dx.doi.org/10.30498/IJB.2021.2631
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author Shahrabi Farahani, Mahboube
Saraygord-Afshari, Neda
M Farajollahi, Mohammad
author_facet Shahrabi Farahani, Mahboube
Saraygord-Afshari, Neda
M Farajollahi, Mohammad
author_sort Shahrabi Farahani, Mahboube
collection PubMed
description BACKGROUND: The unique expression pattern of prostate stem cell antigen (PSCA) in a number of prevalent neoplasms has made the antigen a great target for cancer researches, and many clinical methods have been developed based on the application of this tumor marker. Hence, optimal PSCA laboratory production can be considered a hallmark for many researchers. OBJECTIVE: An analytical study was designed to improve the quality and quantity of PSCA production. MATERIALS AND METHODS: The effects of different compositions of lysis buffers and some ultrasound durations were assessed by calculation of the protein recovery followed by PSCA specific blotting experiments. Then, based on the results of the web-based characterization, interference removal, followed by re-solubilization of the protein in various buffers, was designed, applied, and assessed. RESULTS: Since the selection of an appropriate methodology depends merely on the research purposes, we tried to discuss the pros and cons of the investigated methods according to the hydrophobic nature of PSCA as well as its dramatic tendency to aggregate in the form of inclusion bodies in the expression hosts. CONCLUSIONS: We introduced a newly designed method to fit the delicate immunological surveys and overcome some limiting factors in PSCA production.
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spelling pubmed-83581722021-08-24 Optimizing the Preparation Procedure of Recombinant PSCA, as a Practical Biomarker in Prostate Cancer Shahrabi Farahani, Mahboube Saraygord-Afshari, Neda M Farajollahi, Mohammad Iran J Biotechnol Research Article BACKGROUND: The unique expression pattern of prostate stem cell antigen (PSCA) in a number of prevalent neoplasms has made the antigen a great target for cancer researches, and many clinical methods have been developed based on the application of this tumor marker. Hence, optimal PSCA laboratory production can be considered a hallmark for many researchers. OBJECTIVE: An analytical study was designed to improve the quality and quantity of PSCA production. MATERIALS AND METHODS: The effects of different compositions of lysis buffers and some ultrasound durations were assessed by calculation of the protein recovery followed by PSCA specific blotting experiments. Then, based on the results of the web-based characterization, interference removal, followed by re-solubilization of the protein in various buffers, was designed, applied, and assessed. RESULTS: Since the selection of an appropriate methodology depends merely on the research purposes, we tried to discuss the pros and cons of the investigated methods according to the hydrophobic nature of PSCA as well as its dramatic tendency to aggregate in the form of inclusion bodies in the expression hosts. CONCLUSIONS: We introduced a newly designed method to fit the delicate immunological surveys and overcome some limiting factors in PSCA production. National Institute of Genetic Engineering and Biotechnology 2021-04-01 /pmc/articles/PMC8358172/ /pubmed/34435055 http://dx.doi.org/10.30498/IJB.2021.2631 Text en Copyright: © 2021 The Author(s); Published by Iranian Journal of Biotechnology https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 Unported License, ( http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shahrabi Farahani, Mahboube
Saraygord-Afshari, Neda
M Farajollahi, Mohammad
Optimizing the Preparation Procedure of Recombinant PSCA, as a Practical Biomarker in Prostate Cancer
title Optimizing the Preparation Procedure of Recombinant PSCA, as a Practical Biomarker in Prostate Cancer
title_full Optimizing the Preparation Procedure of Recombinant PSCA, as a Practical Biomarker in Prostate Cancer
title_fullStr Optimizing the Preparation Procedure of Recombinant PSCA, as a Practical Biomarker in Prostate Cancer
title_full_unstemmed Optimizing the Preparation Procedure of Recombinant PSCA, as a Practical Biomarker in Prostate Cancer
title_short Optimizing the Preparation Procedure of Recombinant PSCA, as a Practical Biomarker in Prostate Cancer
title_sort optimizing the preparation procedure of recombinant psca, as a practical biomarker in prostate cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358172/
https://www.ncbi.nlm.nih.gov/pubmed/34435055
http://dx.doi.org/10.30498/IJB.2021.2631
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