CEACAM1 Activation by CbpF-Expressing E. coli

Recent studies on the oral, anaerobic, gram-negative bacterium Fusobacterium nucleatum revealed its presence and involvement in colorectal, esophageal and breast cancer. We previously demonstrated that F. nucleatum binds and activates the human inhibitory receptors TIGIT and CEACAM1 leading to inhib...

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Autores principales: Shhadeh, Amjad, Galaski, Johanna, Alon-Maimon, Tamar, Fahoum, Jamal, Wiener, Reuven, Slade, Daniel J., Mandelboim, Ofer, Bachrach, Gilad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Cellular and Infection Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358318/
https://www.ncbi.nlm.nih.gov/pubmed/34395310
http://dx.doi.org/10.3389/fcimb.2021.699015
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author Shhadeh, Amjad
Galaski, Johanna
Alon-Maimon, Tamar
Fahoum, Jamal
Wiener, Reuven
Slade, Daniel J.
Mandelboim, Ofer
Bachrach, Gilad
author_facet Shhadeh, Amjad
Galaski, Johanna
Alon-Maimon, Tamar
Fahoum, Jamal
Wiener, Reuven
Slade, Daniel J.
Mandelboim, Ofer
Bachrach, Gilad
author_sort Shhadeh, Amjad
collection PubMed
description Recent studies on the oral, anaerobic, gram-negative bacterium Fusobacterium nucleatum revealed its presence and involvement in colorectal, esophageal and breast cancer. We previously demonstrated that F. nucleatum binds and activates the human inhibitory receptors TIGIT and CEACAM1 leading to inhibition of T and NK cell anti-tumor immunity. CEACAM1 was found to be bound and activated by the fusobacterial trimeric autotransporter adhesin CbpF. Here we report the generation of a recombinant E. coli expressing full-length CbpF that efficiently binds and activates CEACAM1.
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spelling pubmed-83583182021-08-13 CEACAM1 Activation by CbpF-Expressing E. coli Shhadeh, Amjad Galaski, Johanna Alon-Maimon, Tamar Fahoum, Jamal Wiener, Reuven Slade, Daniel J. Mandelboim, Ofer Bachrach, Gilad Front Cell Infect Microbiol Cellular and Infection Microbiology Recent studies on the oral, anaerobic, gram-negative bacterium Fusobacterium nucleatum revealed its presence and involvement in colorectal, esophageal and breast cancer. We previously demonstrated that F. nucleatum binds and activates the human inhibitory receptors TIGIT and CEACAM1 leading to inhibition of T and NK cell anti-tumor immunity. CEACAM1 was found to be bound and activated by the fusobacterial trimeric autotransporter adhesin CbpF. Here we report the generation of a recombinant E. coli expressing full-length CbpF that efficiently binds and activates CEACAM1. Frontiers Media S.A. 2021-07-29 /pmc/articles/PMC8358318/ /pubmed/34395310 http://dx.doi.org/10.3389/fcimb.2021.699015 Text en Copyright © 2021 Shhadeh, Galaski, Alon-Maimon, Fahoum, Wiener, Slade, Mandelboim and Bachrach https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Shhadeh, Amjad
Galaski, Johanna
Alon-Maimon, Tamar
Fahoum, Jamal
Wiener, Reuven
Slade, Daniel J.
Mandelboim, Ofer
Bachrach, Gilad
CEACAM1 Activation by CbpF-Expressing E. coli
title CEACAM1 Activation by CbpF-Expressing E. coli
title_full CEACAM1 Activation by CbpF-Expressing E. coli
title_fullStr CEACAM1 Activation by CbpF-Expressing E. coli
title_full_unstemmed CEACAM1 Activation by CbpF-Expressing E. coli
title_short CEACAM1 Activation by CbpF-Expressing E. coli
title_sort ceacam1 activation by cbpf-expressing e. coli
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358318/
https://www.ncbi.nlm.nih.gov/pubmed/34395310
http://dx.doi.org/10.3389/fcimb.2021.699015
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