Fluorescent in situ mRNA detection in the adult mouse cochlea

Ossification and the delicateness of the cochlear duct make histologic assessments of the mature cochlea a challenging endeavor. Treatments to soften the bone facilitate sectioning and dissection of the cochlear duct but limit in situ mRNA detection in such specimens. Here, we provide a protocol for...

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Detalles Bibliográficos
Autores principales: Diaz, Giovanni H., Heller, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358468/
https://www.ncbi.nlm.nih.gov/pubmed/34401777
http://dx.doi.org/10.1016/j.xpro.2021.100711
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author Diaz, Giovanni H.
Heller, Stefan
author_facet Diaz, Giovanni H.
Heller, Stefan
author_sort Diaz, Giovanni H.
collection PubMed
description Ossification and the delicateness of the cochlear duct make histologic assessments of the mature cochlea a challenging endeavor. Treatments to soften the bone facilitate sectioning and dissection of the cochlear duct but limit in situ mRNA detection in such specimens. Here, we provide a protocol for in situ mRNA detection using hybridization chain reaction in whole-mount preparations of the adult mouse cochlea. We show examples for multi-probe detection of different mRNAs and describe combination of this method with conventional immunohistochemistry.
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spelling pubmed-83584682021-08-15 Fluorescent in situ mRNA detection in the adult mouse cochlea Diaz, Giovanni H. Heller, Stefan STAR Protoc Protocol Ossification and the delicateness of the cochlear duct make histologic assessments of the mature cochlea a challenging endeavor. Treatments to soften the bone facilitate sectioning and dissection of the cochlear duct but limit in situ mRNA detection in such specimens. Here, we provide a protocol for in situ mRNA detection using hybridization chain reaction in whole-mount preparations of the adult mouse cochlea. We show examples for multi-probe detection of different mRNAs and describe combination of this method with conventional immunohistochemistry. Elsevier 2021-08-07 /pmc/articles/PMC8358468/ /pubmed/34401777 http://dx.doi.org/10.1016/j.xpro.2021.100711 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Diaz, Giovanni H.
Heller, Stefan
Fluorescent in situ mRNA detection in the adult mouse cochlea
title Fluorescent in situ mRNA detection in the adult mouse cochlea
title_full Fluorescent in situ mRNA detection in the adult mouse cochlea
title_fullStr Fluorescent in situ mRNA detection in the adult mouse cochlea
title_full_unstemmed Fluorescent in situ mRNA detection in the adult mouse cochlea
title_short Fluorescent in situ mRNA detection in the adult mouse cochlea
title_sort fluorescent in situ mrna detection in the adult mouse cochlea
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358468/
https://www.ncbi.nlm.nih.gov/pubmed/34401777
http://dx.doi.org/10.1016/j.xpro.2021.100711
work_keys_str_mv AT diazgiovannih fluorescentinsitumrnadetectionintheadultmousecochlea
AT hellerstefan fluorescentinsitumrnadetectionintheadultmousecochlea

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