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Tissue remodelling and increased DNA damage in patients with incompetent valves in chronic venous insufficiency

Chronic venous insufficiency (CVI), in which blood return to the heart is impaired, is a prevalent condition worldwide. Valve incompetence is a complication of CVI that results in blood reflux, thereby aggravating venous hypertension. While CVI has a complex course and is known to produce alteration...

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Autores principales: Ortega, Miguel A., Fraile‐Martínez, Oscar, García‐Montero, Cielo, Pekarek, Leonel, Alvarez‐Mon, Miguel A., Guijarro, Luis G., del Carmen Boyano, Maria, Sainz, Felipe, Álvarez‐Mon, Melchor, Buján, Julia, García‐Honduvilla, Natalio, Asúnsolo, Ángel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358866/
https://www.ncbi.nlm.nih.gov/pubmed/34148301
http://dx.doi.org/10.1111/jcmm.16711
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author Ortega, Miguel A.
Fraile‐Martínez, Oscar
García‐Montero, Cielo
Pekarek, Leonel
Alvarez‐Mon, Miguel A.
Guijarro, Luis G.
del Carmen Boyano, Maria
Sainz, Felipe
Álvarez‐Mon, Melchor
Buján, Julia
García‐Honduvilla, Natalio
Asúnsolo, Ángel
author_facet Ortega, Miguel A.
Fraile‐Martínez, Oscar
García‐Montero, Cielo
Pekarek, Leonel
Alvarez‐Mon, Miguel A.
Guijarro, Luis G.
del Carmen Boyano, Maria
Sainz, Felipe
Álvarez‐Mon, Melchor
Buján, Julia
García‐Honduvilla, Natalio
Asúnsolo, Ángel
author_sort Ortega, Miguel A.
collection PubMed
description Chronic venous insufficiency (CVI), in which blood return to the heart is impaired, is a prevalent condition worldwide. Valve incompetence is a complication of CVI that results in blood reflux, thereby aggravating venous hypertension. While CVI has a complex course and is known to produce alterations in the vein wall, the underlying pathological mechanisms remain unclear. This study examined the presence of DNA damage, pro‐inflammatory cytokines and extracellular matrix remodelling in CVI‐related valve incompetence. One hundred and ten patients with CVI were reviewed and divided into four groups according to age (<50 and ≥50 years) and a clinical diagnosis of venous reflux indicating venous system valve incompetence (R) (n = 81) or no reflux (NR) (n = 29). In vein specimens (greater saphenous vein) from each group, PARP, IL‐17, COL‐I, COL‐III, MMP‐2 and TIMP‐2 expression levels were determined by RT‐qPCR and immunohistochemistry. The younger patients with valve incompetence showed significantly higher PARP, IL‐17, COL‐I, COL‐III, MMP‐2 and reduced TIMP‐2 expression levels and a higher COL‐I/III ratio. Young CVI patients with venous reflux suffer chronic DNA damage, with consequences at both the local tissue and systemic levels, possibly associated with ageing.
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spelling pubmed-83588662021-08-15 Tissue remodelling and increased DNA damage in patients with incompetent valves in chronic venous insufficiency Ortega, Miguel A. Fraile‐Martínez, Oscar García‐Montero, Cielo Pekarek, Leonel Alvarez‐Mon, Miguel A. Guijarro, Luis G. del Carmen Boyano, Maria Sainz, Felipe Álvarez‐Mon, Melchor Buján, Julia García‐Honduvilla, Natalio Asúnsolo, Ángel J Cell Mol Med Original Articles Chronic venous insufficiency (CVI), in which blood return to the heart is impaired, is a prevalent condition worldwide. Valve incompetence is a complication of CVI that results in blood reflux, thereby aggravating venous hypertension. While CVI has a complex course and is known to produce alterations in the vein wall, the underlying pathological mechanisms remain unclear. This study examined the presence of DNA damage, pro‐inflammatory cytokines and extracellular matrix remodelling in CVI‐related valve incompetence. One hundred and ten patients with CVI were reviewed and divided into four groups according to age (<50 and ≥50 years) and a clinical diagnosis of venous reflux indicating venous system valve incompetence (R) (n = 81) or no reflux (NR) (n = 29). In vein specimens (greater saphenous vein) from each group, PARP, IL‐17, COL‐I, COL‐III, MMP‐2 and TIMP‐2 expression levels were determined by RT‐qPCR and immunohistochemistry. The younger patients with valve incompetence showed significantly higher PARP, IL‐17, COL‐I, COL‐III, MMP‐2 and reduced TIMP‐2 expression levels and a higher COL‐I/III ratio. Young CVI patients with venous reflux suffer chronic DNA damage, with consequences at both the local tissue and systemic levels, possibly associated with ageing. John Wiley and Sons Inc. 2021-06-20 2021-08 /pmc/articles/PMC8358866/ /pubmed/34148301 http://dx.doi.org/10.1111/jcmm.16711 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Ortega, Miguel A.
Fraile‐Martínez, Oscar
García‐Montero, Cielo
Pekarek, Leonel
Alvarez‐Mon, Miguel A.
Guijarro, Luis G.
del Carmen Boyano, Maria
Sainz, Felipe
Álvarez‐Mon, Melchor
Buján, Julia
García‐Honduvilla, Natalio
Asúnsolo, Ángel
Tissue remodelling and increased DNA damage in patients with incompetent valves in chronic venous insufficiency
title Tissue remodelling and increased DNA damage in patients with incompetent valves in chronic venous insufficiency
title_full Tissue remodelling and increased DNA damage in patients with incompetent valves in chronic venous insufficiency
title_fullStr Tissue remodelling and increased DNA damage in patients with incompetent valves in chronic venous insufficiency
title_full_unstemmed Tissue remodelling and increased DNA damage in patients with incompetent valves in chronic venous insufficiency
title_short Tissue remodelling and increased DNA damage in patients with incompetent valves in chronic venous insufficiency
title_sort tissue remodelling and increased dna damage in patients with incompetent valves in chronic venous insufficiency
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358866/
https://www.ncbi.nlm.nih.gov/pubmed/34148301
http://dx.doi.org/10.1111/jcmm.16711
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