Cargando…

The lncRNA HOTAIR regulates autophagy and affects lipopolysaccharide‐induced acute lung injury through the miR‐17‐5p/ATG2/ATG7/ATG16 axis

Long non‐coding ribonucleic acids (lncRNAs) play critical roles in acute lung injury (ALI). We aimed to explore the involvement of lncRNA HOX transcript antisense intergenic ribonucleic acid (HOTAIR) in regulating autophagy in lipopolysaccharide (LPS)‐induced ALI. We obtained 1289 differentially exp...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Yujun, Liang, Zhike, He, Hua, Huang, Xiaomei, Mo, Zexun, Tan, Jinwen, Guo, Weihong, Zhao, Ziwen, Wei, Shuquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358883/
https://www.ncbi.nlm.nih.gov/pubmed/34180119
http://dx.doi.org/10.1111/jcmm.16737
_version_ 1783737431990730752
author Li, Yujun
Liang, Zhike
He, Hua
Huang, Xiaomei
Mo, Zexun
Tan, Jinwen
Guo, Weihong
Zhao, Ziwen
Wei, Shuquan
author_facet Li, Yujun
Liang, Zhike
He, Hua
Huang, Xiaomei
Mo, Zexun
Tan, Jinwen
Guo, Weihong
Zhao, Ziwen
Wei, Shuquan
author_sort Li, Yujun
collection PubMed
description Long non‐coding ribonucleic acids (lncRNAs) play critical roles in acute lung injury (ALI). We aimed to explore the involvement of lncRNA HOX transcript antisense intergenic ribonucleic acid (HOTAIR) in regulating autophagy in lipopolysaccharide (LPS)‐induced ALI. We obtained 1289 differentially expressed lncRNAs or messenger RNAs (mRNAs) via microarray analysis. HOTAIR was significantly upregulated in the LPS stimulation experimental group. HOTAIR knockdown (si‐HOTAIR) promoted cell proliferation in LPS‐stimulated A549 and BEAS‐2B cells, suppressing the protein expression of autophagy marker light chain 3B and Beclin‐1. Inhibition of HOTAIR suppressed LPS‐induced cell autophagy, apoptosis and arrested cells in the G0/G1 phase prior to S phase entry. Further, si‐HOTAIR alleviated LPS‐induced lung injury in vivo. We predicted the micro‐ribonucleic acid miR‐17‐5p to target HOTAIR and confirmed this via RNA pull‐down and dual luciferase reporter assays. miR‐17‐5p inhibitor treatment reversed the HOTAIR‐mediated effects on autophagy, apoptosis, cell proliferation and cell cycle. Finally, we predicted autophagy‐related genes (ATGs) ATG2, ATG7 and ATG16 as targets of miR‐17‐5p, which reversed their HOTAIR‐mediated protein upregulation in LPS‐stimulated A549 and BEAS‐2B cells. Taken together, our results indicate that HOTAIR regulated apoptosis, the cell cycle, proliferation and autophagy through the miR‐17‐5p/ATG2/ATG7/ATG16 axis, thus driving LPS‐induced ALI.
format Online
Article
Text
id pubmed-8358883
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-83588832021-08-15 The lncRNA HOTAIR regulates autophagy and affects lipopolysaccharide‐induced acute lung injury through the miR‐17‐5p/ATG2/ATG7/ATG16 axis Li, Yujun Liang, Zhike He, Hua Huang, Xiaomei Mo, Zexun Tan, Jinwen Guo, Weihong Zhao, Ziwen Wei, Shuquan J Cell Mol Med Original Articles Long non‐coding ribonucleic acids (lncRNAs) play critical roles in acute lung injury (ALI). We aimed to explore the involvement of lncRNA HOX transcript antisense intergenic ribonucleic acid (HOTAIR) in regulating autophagy in lipopolysaccharide (LPS)‐induced ALI. We obtained 1289 differentially expressed lncRNAs or messenger RNAs (mRNAs) via microarray analysis. HOTAIR was significantly upregulated in the LPS stimulation experimental group. HOTAIR knockdown (si‐HOTAIR) promoted cell proliferation in LPS‐stimulated A549 and BEAS‐2B cells, suppressing the protein expression of autophagy marker light chain 3B and Beclin‐1. Inhibition of HOTAIR suppressed LPS‐induced cell autophagy, apoptosis and arrested cells in the G0/G1 phase prior to S phase entry. Further, si‐HOTAIR alleviated LPS‐induced lung injury in vivo. We predicted the micro‐ribonucleic acid miR‐17‐5p to target HOTAIR and confirmed this via RNA pull‐down and dual luciferase reporter assays. miR‐17‐5p inhibitor treatment reversed the HOTAIR‐mediated effects on autophagy, apoptosis, cell proliferation and cell cycle. Finally, we predicted autophagy‐related genes (ATGs) ATG2, ATG7 and ATG16 as targets of miR‐17‐5p, which reversed their HOTAIR‐mediated protein upregulation in LPS‐stimulated A549 and BEAS‐2B cells. Taken together, our results indicate that HOTAIR regulated apoptosis, the cell cycle, proliferation and autophagy through the miR‐17‐5p/ATG2/ATG7/ATG16 axis, thus driving LPS‐induced ALI. John Wiley and Sons Inc. 2021-06-27 2021-08 /pmc/articles/PMC8358883/ /pubmed/34180119 http://dx.doi.org/10.1111/jcmm.16737 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Li, Yujun
Liang, Zhike
He, Hua
Huang, Xiaomei
Mo, Zexun
Tan, Jinwen
Guo, Weihong
Zhao, Ziwen
Wei, Shuquan
The lncRNA HOTAIR regulates autophagy and affects lipopolysaccharide‐induced acute lung injury through the miR‐17‐5p/ATG2/ATG7/ATG16 axis
title The lncRNA HOTAIR regulates autophagy and affects lipopolysaccharide‐induced acute lung injury through the miR‐17‐5p/ATG2/ATG7/ATG16 axis
title_full The lncRNA HOTAIR regulates autophagy and affects lipopolysaccharide‐induced acute lung injury through the miR‐17‐5p/ATG2/ATG7/ATG16 axis
title_fullStr The lncRNA HOTAIR regulates autophagy and affects lipopolysaccharide‐induced acute lung injury through the miR‐17‐5p/ATG2/ATG7/ATG16 axis
title_full_unstemmed The lncRNA HOTAIR regulates autophagy and affects lipopolysaccharide‐induced acute lung injury through the miR‐17‐5p/ATG2/ATG7/ATG16 axis
title_short The lncRNA HOTAIR regulates autophagy and affects lipopolysaccharide‐induced acute lung injury through the miR‐17‐5p/ATG2/ATG7/ATG16 axis
title_sort lncrna hotair regulates autophagy and affects lipopolysaccharide‐induced acute lung injury through the mir‐17‐5p/atg2/atg7/atg16 axis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358883/
https://www.ncbi.nlm.nih.gov/pubmed/34180119
http://dx.doi.org/10.1111/jcmm.16737
work_keys_str_mv AT liyujun thelncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT liangzhike thelncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT hehua thelncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT huangxiaomei thelncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT mozexun thelncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT tanjinwen thelncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT guoweihong thelncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT zhaoziwen thelncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT weishuquan thelncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT liyujun lncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT liangzhike lncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT hehua lncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT huangxiaomei lncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT mozexun lncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT tanjinwen lncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT guoweihong lncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT zhaoziwen lncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis
AT weishuquan lncrnahotairregulatesautophagyandaffectslipopolysaccharideinducedacutelunginjurythroughthemir175patg2atg7atg16axis