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Transcriptome Analysis of Peripheral Blood Mononuclear Cells Reveals Distinct Immune Response in Asymptomatic and Re-Detectable Positive COVID-19 Patients

The existence of asymptomatic and re-detectable positive coronavirus disease 2019 (COVID-19) patients presents the disease control challenges of COVID-19. Most studies on immune responses in COVID-19 have focused on moderately or severely symptomatic patients; however, little is known about the immu...

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Autores principales: Zhang, Jiaqi, Lin, Dongzi, Li, Kui, Ding, Xiangming, Li, Lin, Liu, Yuntao, Liu, Dongdong, Lin, Jing, Teng, Xiangyun, Li, Yizhe, Liu, Ming, Shen, Jian, Wang, Xiaodan, He, Dan, Shi, Yaling, Wang, Dawei, Xu, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359015/
https://www.ncbi.nlm.nih.gov/pubmed/34394120
http://dx.doi.org/10.3389/fimmu.2021.716075
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author Zhang, Jiaqi
Lin, Dongzi
Li, Kui
Ding, Xiangming
Li, Lin
Liu, Yuntao
Liu, Dongdong
Lin, Jing
Teng, Xiangyun
Li, Yizhe
Liu, Ming
Shen, Jian
Wang, Xiaodan
He, Dan
Shi, Yaling
Wang, Dawei
Xu, Jianhua
author_facet Zhang, Jiaqi
Lin, Dongzi
Li, Kui
Ding, Xiangming
Li, Lin
Liu, Yuntao
Liu, Dongdong
Lin, Jing
Teng, Xiangyun
Li, Yizhe
Liu, Ming
Shen, Jian
Wang, Xiaodan
He, Dan
Shi, Yaling
Wang, Dawei
Xu, Jianhua
author_sort Zhang, Jiaqi
collection PubMed
description The existence of asymptomatic and re-detectable positive coronavirus disease 2019 (COVID-19) patients presents the disease control challenges of COVID-19. Most studies on immune responses in COVID-19 have focused on moderately or severely symptomatic patients; however, little is known about the immune response in asymptomatic and re-detectable positive (RP) patients. Here we performed a comprehensive analysis of the transcriptomic profiles of peripheral blood mononuclear cells (PBMCs) from 48 COVID-19 patients which included 8 asymptomatic, 13 symptomatic, 15 recovered and 12 RP patients. The weighted gene co-expression network analysis (WGCNA) identified six co-expression modules, of which the turquoise module was positively correlated with the asymptomatic, symptomatic, and recovered COVID-19 patients. The red module positively correlated with symptomatic patients only and the blue and brown modules positively correlated with the RP patients. The analysis by single sample gene set enrichment analysis (ssGSEA) revealed a lower level of IFN response and complement activation in the asymptomatic patients compared with the symptomatic, indicating a weaker immune response of the PBMCs in the asymptomatic patients. In addition, gene set enrichment analysis (GSEA) analysis showed the enrichment of TNFα/NF-κB and influenza infection in the RP patients compared with the recovered patients, indicating a hyper-inflammatory immune response in the PBMC of RP patients. Thus our findings could extend our understanding of host immune response during the progression of COVID-19 disease and assist clinical management and the immunotherapy development for COVID-19.
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spelling pubmed-83590152021-08-13 Transcriptome Analysis of Peripheral Blood Mononuclear Cells Reveals Distinct Immune Response in Asymptomatic and Re-Detectable Positive COVID-19 Patients Zhang, Jiaqi Lin, Dongzi Li, Kui Ding, Xiangming Li, Lin Liu, Yuntao Liu, Dongdong Lin, Jing Teng, Xiangyun Li, Yizhe Liu, Ming Shen, Jian Wang, Xiaodan He, Dan Shi, Yaling Wang, Dawei Xu, Jianhua Front Immunol Immunology The existence of asymptomatic and re-detectable positive coronavirus disease 2019 (COVID-19) patients presents the disease control challenges of COVID-19. Most studies on immune responses in COVID-19 have focused on moderately or severely symptomatic patients; however, little is known about the immune response in asymptomatic and re-detectable positive (RP) patients. Here we performed a comprehensive analysis of the transcriptomic profiles of peripheral blood mononuclear cells (PBMCs) from 48 COVID-19 patients which included 8 asymptomatic, 13 symptomatic, 15 recovered and 12 RP patients. The weighted gene co-expression network analysis (WGCNA) identified six co-expression modules, of which the turquoise module was positively correlated with the asymptomatic, symptomatic, and recovered COVID-19 patients. The red module positively correlated with symptomatic patients only and the blue and brown modules positively correlated with the RP patients. The analysis by single sample gene set enrichment analysis (ssGSEA) revealed a lower level of IFN response and complement activation in the asymptomatic patients compared with the symptomatic, indicating a weaker immune response of the PBMCs in the asymptomatic patients. In addition, gene set enrichment analysis (GSEA) analysis showed the enrichment of TNFα/NF-κB and influenza infection in the RP patients compared with the recovered patients, indicating a hyper-inflammatory immune response in the PBMC of RP patients. Thus our findings could extend our understanding of host immune response during the progression of COVID-19 disease and assist clinical management and the immunotherapy development for COVID-19. Frontiers Media S.A. 2021-07-29 /pmc/articles/PMC8359015/ /pubmed/34394120 http://dx.doi.org/10.3389/fimmu.2021.716075 Text en Copyright © 2021 Zhang, Lin, Li, Ding, Li, Liu, Liu, Lin, Teng, Li, Liu, Shen, Wang, He, Shi, Wang and Xu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Zhang, Jiaqi
Lin, Dongzi
Li, Kui
Ding, Xiangming
Li, Lin
Liu, Yuntao
Liu, Dongdong
Lin, Jing
Teng, Xiangyun
Li, Yizhe
Liu, Ming
Shen, Jian
Wang, Xiaodan
He, Dan
Shi, Yaling
Wang, Dawei
Xu, Jianhua
Transcriptome Analysis of Peripheral Blood Mononuclear Cells Reveals Distinct Immune Response in Asymptomatic and Re-Detectable Positive COVID-19 Patients
title Transcriptome Analysis of Peripheral Blood Mononuclear Cells Reveals Distinct Immune Response in Asymptomatic and Re-Detectable Positive COVID-19 Patients
title_full Transcriptome Analysis of Peripheral Blood Mononuclear Cells Reveals Distinct Immune Response in Asymptomatic and Re-Detectable Positive COVID-19 Patients
title_fullStr Transcriptome Analysis of Peripheral Blood Mononuclear Cells Reveals Distinct Immune Response in Asymptomatic and Re-Detectable Positive COVID-19 Patients
title_full_unstemmed Transcriptome Analysis of Peripheral Blood Mononuclear Cells Reveals Distinct Immune Response in Asymptomatic and Re-Detectable Positive COVID-19 Patients
title_short Transcriptome Analysis of Peripheral Blood Mononuclear Cells Reveals Distinct Immune Response in Asymptomatic and Re-Detectable Positive COVID-19 Patients
title_sort transcriptome analysis of peripheral blood mononuclear cells reveals distinct immune response in asymptomatic and re-detectable positive covid-19 patients
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359015/
https://www.ncbi.nlm.nih.gov/pubmed/34394120
http://dx.doi.org/10.3389/fimmu.2021.716075
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