Cargando…
Summary of reference chemicals evaluated by the fish short‐term reproduction assay, OECD TG229, using Japanese Medaka, Oryzias latipes
Under the Organisation for Economic Co‐operation and Development (OECD), the Ministry of the Environment of Japan (MOE) added Japanese medaka (Oryzias latipes) to the test guideline fish short‐term reproduction assay (FSTRA) developed by the United States Environmental Protection Agency (US EPA) usi...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359193/ https://www.ncbi.nlm.nih.gov/pubmed/33486801 http://dx.doi.org/10.1002/jat.4104 |
_version_ | 1783737494022389760 |
---|---|
author | Onishi, Yuta Tatarazako, Norihisa Koshio, Masaaki Okamura, Tetsuro Watanabe, Haruna Sawai, Atsushi Yamamoto, Jun Ishikawa, Hidenori Sato, Tomomi Kawashima, Yukio Yamazaki, Kunihiko Iguchi, Taisen |
author_facet | Onishi, Yuta Tatarazako, Norihisa Koshio, Masaaki Okamura, Tetsuro Watanabe, Haruna Sawai, Atsushi Yamamoto, Jun Ishikawa, Hidenori Sato, Tomomi Kawashima, Yukio Yamazaki, Kunihiko Iguchi, Taisen |
author_sort | Onishi, Yuta |
collection | PubMed |
description | Under the Organisation for Economic Co‐operation and Development (OECD), the Ministry of the Environment of Japan (MOE) added Japanese medaka (Oryzias latipes) to the test guideline fish short‐term reproduction assay (FSTRA) developed by the United States Environmental Protection Agency (US EPA) using fathead minnow (Pimephales promelas). The FSTRA was designed to detect endocrine disrupting effects of chemicals interacting with the hypothalamic–pituitary–gonadal axis (HPG axis) such as agonists or antagonists on the estrogen receptor (Esr) and/or the androgen receptor (AR) and steroidogenesis inhibitors. We conducted the FSTRA with Japanese medaka, in accordance with OECD test guideline number 229 (TG229), for 16 chemicals including four Esr agonists, two Esr antagonists, three AR agonists, two AR antagonists, two steroidogenesis inhibitors, two progesterone receptor agonists, and a negative substance, and evaluated the usability and the validity of the FSTRA (TG229) protocol. In addition, in vitro reporter gene assays (RGAs) using Esr1 and ARβ of Japanese medaka were performed for the 16 chemicals, to support the interpretation of the in vivo effects observed in the FSTRA. In the present study, all the test chemicals, except an antiandrogenic chemical and a weak Esr agonist, significantly reduced the reproductive status of the test fish, that is, fecundity or fertility, at concentrations where no overt toxicity was observed. Moreover, vitellogenin (VTG) induction in males and formation of secondary sex characteristics (SSC), papillary processes on the anal fin, in females was sensitive endpoints to Esr and AR agonistic effects, respectively, and might be indicators of the effect concentrations in long‐term exposure. Overall, it is suggested that the in vivo FSTRA supported by in vitro RGA data can adequately detect effects on the test fish, O. latipes, and probably identify the mode of action (MOA) of the chemicals tested. |
format | Online Article Text |
id | pubmed-8359193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83591932021-08-17 Summary of reference chemicals evaluated by the fish short‐term reproduction assay, OECD TG229, using Japanese Medaka, Oryzias latipes Onishi, Yuta Tatarazako, Norihisa Koshio, Masaaki Okamura, Tetsuro Watanabe, Haruna Sawai, Atsushi Yamamoto, Jun Ishikawa, Hidenori Sato, Tomomi Kawashima, Yukio Yamazaki, Kunihiko Iguchi, Taisen J Appl Toxicol Research Articles Under the Organisation for Economic Co‐operation and Development (OECD), the Ministry of the Environment of Japan (MOE) added Japanese medaka (Oryzias latipes) to the test guideline fish short‐term reproduction assay (FSTRA) developed by the United States Environmental Protection Agency (US EPA) using fathead minnow (Pimephales promelas). The FSTRA was designed to detect endocrine disrupting effects of chemicals interacting with the hypothalamic–pituitary–gonadal axis (HPG axis) such as agonists or antagonists on the estrogen receptor (Esr) and/or the androgen receptor (AR) and steroidogenesis inhibitors. We conducted the FSTRA with Japanese medaka, in accordance with OECD test guideline number 229 (TG229), for 16 chemicals including four Esr agonists, two Esr antagonists, three AR agonists, two AR antagonists, two steroidogenesis inhibitors, two progesterone receptor agonists, and a negative substance, and evaluated the usability and the validity of the FSTRA (TG229) protocol. In addition, in vitro reporter gene assays (RGAs) using Esr1 and ARβ of Japanese medaka were performed for the 16 chemicals, to support the interpretation of the in vivo effects observed in the FSTRA. In the present study, all the test chemicals, except an antiandrogenic chemical and a weak Esr agonist, significantly reduced the reproductive status of the test fish, that is, fecundity or fertility, at concentrations where no overt toxicity was observed. Moreover, vitellogenin (VTG) induction in males and formation of secondary sex characteristics (SSC), papillary processes on the anal fin, in females was sensitive endpoints to Esr and AR agonistic effects, respectively, and might be indicators of the effect concentrations in long‐term exposure. Overall, it is suggested that the in vivo FSTRA supported by in vitro RGA data can adequately detect effects on the test fish, O. latipes, and probably identify the mode of action (MOA) of the chemicals tested. John Wiley and Sons Inc. 2021-01-24 2021-08 /pmc/articles/PMC8359193/ /pubmed/33486801 http://dx.doi.org/10.1002/jat.4104 Text en © 2021 The Authors. Journal of Applied Toxicology published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Onishi, Yuta Tatarazako, Norihisa Koshio, Masaaki Okamura, Tetsuro Watanabe, Haruna Sawai, Atsushi Yamamoto, Jun Ishikawa, Hidenori Sato, Tomomi Kawashima, Yukio Yamazaki, Kunihiko Iguchi, Taisen Summary of reference chemicals evaluated by the fish short‐term reproduction assay, OECD TG229, using Japanese Medaka, Oryzias latipes |
title | Summary of reference chemicals evaluated by the fish short‐term reproduction assay, OECD TG229, using Japanese Medaka, Oryzias latipes
|
title_full | Summary of reference chemicals evaluated by the fish short‐term reproduction assay, OECD TG229, using Japanese Medaka, Oryzias latipes
|
title_fullStr | Summary of reference chemicals evaluated by the fish short‐term reproduction assay, OECD TG229, using Japanese Medaka, Oryzias latipes
|
title_full_unstemmed | Summary of reference chemicals evaluated by the fish short‐term reproduction assay, OECD TG229, using Japanese Medaka, Oryzias latipes
|
title_short | Summary of reference chemicals evaluated by the fish short‐term reproduction assay, OECD TG229, using Japanese Medaka, Oryzias latipes
|
title_sort | summary of reference chemicals evaluated by the fish short‐term reproduction assay, oecd tg229, using japanese medaka, oryzias latipes |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359193/ https://www.ncbi.nlm.nih.gov/pubmed/33486801 http://dx.doi.org/10.1002/jat.4104 |
work_keys_str_mv | AT onishiyuta summaryofreferencechemicalsevaluatedbythefishshorttermreproductionassayoecdtg229usingjapanesemedakaoryziaslatipes AT tatarazakonorihisa summaryofreferencechemicalsevaluatedbythefishshorttermreproductionassayoecdtg229usingjapanesemedakaoryziaslatipes AT koshiomasaaki summaryofreferencechemicalsevaluatedbythefishshorttermreproductionassayoecdtg229usingjapanesemedakaoryziaslatipes AT okamuratetsuro summaryofreferencechemicalsevaluatedbythefishshorttermreproductionassayoecdtg229usingjapanesemedakaoryziaslatipes AT watanabeharuna summaryofreferencechemicalsevaluatedbythefishshorttermreproductionassayoecdtg229usingjapanesemedakaoryziaslatipes AT sawaiatsushi summaryofreferencechemicalsevaluatedbythefishshorttermreproductionassayoecdtg229usingjapanesemedakaoryziaslatipes AT yamamotojun summaryofreferencechemicalsevaluatedbythefishshorttermreproductionassayoecdtg229usingjapanesemedakaoryziaslatipes AT ishikawahidenori summaryofreferencechemicalsevaluatedbythefishshorttermreproductionassayoecdtg229usingjapanesemedakaoryziaslatipes AT satotomomi summaryofreferencechemicalsevaluatedbythefishshorttermreproductionassayoecdtg229usingjapanesemedakaoryziaslatipes AT kawashimayukio summaryofreferencechemicalsevaluatedbythefishshorttermreproductionassayoecdtg229usingjapanesemedakaoryziaslatipes AT yamazakikunihiko summaryofreferencechemicalsevaluatedbythefishshorttermreproductionassayoecdtg229usingjapanesemedakaoryziaslatipes AT iguchitaisen summaryofreferencechemicalsevaluatedbythefishshorttermreproductionassayoecdtg229usingjapanesemedakaoryziaslatipes |