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Label-free methods for optical in vitro characterization of protein–protein interactions
Protein–protein interactions are involved in the regulation and function of the majority of cellular processes. As a result, much effort has been aimed at the development of methodologies capable of quantifying protein–protein interactions, with label-free methods being of particular interest due to...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359934/ https://www.ncbi.nlm.nih.gov/pubmed/34342317 http://dx.doi.org/10.1039/d1cp01072g |
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author | Soltermann, Fabian Struwe, Weston B. Kukura, Philipp |
author_facet | Soltermann, Fabian Struwe, Weston B. Kukura, Philipp |
author_sort | Soltermann, Fabian |
collection | PubMed |
description | Protein–protein interactions are involved in the regulation and function of the majority of cellular processes. As a result, much effort has been aimed at the development of methodologies capable of quantifying protein–protein interactions, with label-free methods being of particular interest due to the associated simplified workflows and minimisation of label-induced perturbations. Here, we review recent advances in optical technologies providing label-free in vitro measurements of affinities and kinetics. We provide an overview and comparison of existing techniques and their principles, discussing advantages, limitations, and recent applications. |
format | Online Article Text |
id | pubmed-8359934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-83599342021-08-25 Label-free methods for optical in vitro characterization of protein–protein interactions Soltermann, Fabian Struwe, Weston B. Kukura, Philipp Phys Chem Chem Phys Chemistry Protein–protein interactions are involved in the regulation and function of the majority of cellular processes. As a result, much effort has been aimed at the development of methodologies capable of quantifying protein–protein interactions, with label-free methods being of particular interest due to the associated simplified workflows and minimisation of label-induced perturbations. Here, we review recent advances in optical technologies providing label-free in vitro measurements of affinities and kinetics. We provide an overview and comparison of existing techniques and their principles, discussing advantages, limitations, and recent applications. The Royal Society of Chemistry 2021-08-03 /pmc/articles/PMC8359934/ /pubmed/34342317 http://dx.doi.org/10.1039/d1cp01072g Text en This journal is © the Owner Societies https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Soltermann, Fabian Struwe, Weston B. Kukura, Philipp Label-free methods for optical in vitro characterization of protein–protein interactions |
title | Label-free methods for optical in vitro characterization of protein–protein interactions |
title_full | Label-free methods for optical in vitro characterization of protein–protein interactions |
title_fullStr | Label-free methods for optical in vitro characterization of protein–protein interactions |
title_full_unstemmed | Label-free methods for optical in vitro characterization of protein–protein interactions |
title_short | Label-free methods for optical in vitro characterization of protein–protein interactions |
title_sort | label-free methods for optical in vitro characterization of protein–protein interactions |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359934/ https://www.ncbi.nlm.nih.gov/pubmed/34342317 http://dx.doi.org/10.1039/d1cp01072g |
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