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Label-free methods for optical in vitro characterization of protein–protein interactions

Protein–protein interactions are involved in the regulation and function of the majority of cellular processes. As a result, much effort has been aimed at the development of methodologies capable of quantifying protein–protein interactions, with label-free methods being of particular interest due to...

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Detalles Bibliográficos
Autores principales: Soltermann, Fabian, Struwe, Weston B., Kukura, Philipp
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359934/
https://www.ncbi.nlm.nih.gov/pubmed/34342317
http://dx.doi.org/10.1039/d1cp01072g
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author Soltermann, Fabian
Struwe, Weston B.
Kukura, Philipp
author_facet Soltermann, Fabian
Struwe, Weston B.
Kukura, Philipp
author_sort Soltermann, Fabian
collection PubMed
description Protein–protein interactions are involved in the regulation and function of the majority of cellular processes. As a result, much effort has been aimed at the development of methodologies capable of quantifying protein–protein interactions, with label-free methods being of particular interest due to the associated simplified workflows and minimisation of label-induced perturbations. Here, we review recent advances in optical technologies providing label-free in vitro measurements of affinities and kinetics. We provide an overview and comparison of existing techniques and their principles, discussing advantages, limitations, and recent applications.
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spelling pubmed-83599342021-08-25 Label-free methods for optical in vitro characterization of protein–protein interactions Soltermann, Fabian Struwe, Weston B. Kukura, Philipp Phys Chem Chem Phys Chemistry Protein–protein interactions are involved in the regulation and function of the majority of cellular processes. As a result, much effort has been aimed at the development of methodologies capable of quantifying protein–protein interactions, with label-free methods being of particular interest due to the associated simplified workflows and minimisation of label-induced perturbations. Here, we review recent advances in optical technologies providing label-free in vitro measurements of affinities and kinetics. We provide an overview and comparison of existing techniques and their principles, discussing advantages, limitations, and recent applications. The Royal Society of Chemistry 2021-08-03 /pmc/articles/PMC8359934/ /pubmed/34342317 http://dx.doi.org/10.1039/d1cp01072g Text en This journal is © the Owner Societies https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Soltermann, Fabian
Struwe, Weston B.
Kukura, Philipp
Label-free methods for optical in vitro characterization of protein–protein interactions
title Label-free methods for optical in vitro characterization of protein–protein interactions
title_full Label-free methods for optical in vitro characterization of protein–protein interactions
title_fullStr Label-free methods for optical in vitro characterization of protein–protein interactions
title_full_unstemmed Label-free methods for optical in vitro characterization of protein–protein interactions
title_short Label-free methods for optical in vitro characterization of protein–protein interactions
title_sort label-free methods for optical in vitro characterization of protein–protein interactions
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359934/
https://www.ncbi.nlm.nih.gov/pubmed/34342317
http://dx.doi.org/10.1039/d1cp01072g
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