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Alteration of type I interferon response is associated with subclinical atherosclerosis in virologically suppressed HIV‐1‐infected male patients

Given human immunodeficiency virus‐1 (HIV‐1)‐infected patients have alterations in the type I interferon (IFN‐I) pathway and are also at elevated risk of atherosclerosis, we evaluated IFN‐I response and subclinical cardiovascular disease (CVD) association in HIV‐1‐infected patients. Transcript level...

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Autores principales: Santinelli, Letizia, De Girolamo, Gabriella, Borrazzo, Cristian, Vassalini, Paolo, Pinacchio, Claudia, Cavallari, Eugenio Nelson, Statzu, Maura, Frasca, Federica, Scordio, Mirko, Bitossi, Camilla, Viscido, Agnese, Ceccarelli, Giancarlo, Mancone, Massimo, Mastroianni, Claudio Maria, Antonelli, Guido, d'Ettorre, Gabriella, Scagnolari, Carolina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360015/
https://www.ncbi.nlm.nih.gov/pubmed/33913525
http://dx.doi.org/10.1002/jmv.27028
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author Santinelli, Letizia
De Girolamo, Gabriella
Borrazzo, Cristian
Vassalini, Paolo
Pinacchio, Claudia
Cavallari, Eugenio Nelson
Statzu, Maura
Frasca, Federica
Scordio, Mirko
Bitossi, Camilla
Viscido, Agnese
Ceccarelli, Giancarlo
Mancone, Massimo
Mastroianni, Claudio Maria
Antonelli, Guido
d'Ettorre, Gabriella
Scagnolari, Carolina
author_facet Santinelli, Letizia
De Girolamo, Gabriella
Borrazzo, Cristian
Vassalini, Paolo
Pinacchio, Claudia
Cavallari, Eugenio Nelson
Statzu, Maura
Frasca, Federica
Scordio, Mirko
Bitossi, Camilla
Viscido, Agnese
Ceccarelli, Giancarlo
Mancone, Massimo
Mastroianni, Claudio Maria
Antonelli, Guido
d'Ettorre, Gabriella
Scagnolari, Carolina
author_sort Santinelli, Letizia
collection PubMed
description Given human immunodeficiency virus‐1 (HIV‐1)‐infected patients have alterations in the type I interferon (IFN‐I) pathway and are also at elevated risk of atherosclerosis, we evaluated IFN‐I response and subclinical cardiovascular disease (CVD) association in HIV‐1‐infected patients. Transcript levels of IFN‐α/β and IFN‐stimulated gene 56 (ISG56) were evaluated by RT/real‐time PCR in peripheral blood mononuclear cells collected from asymptomatic HIV‐1‐positive male patients at high risk of developing CVD (n = 34) and healthy subjects (n = 21). Stenosis degree (≥ or <50%), calcium volume score, calcium Agatston score, and myocardial extracellular volume were examined by coronary computerized tomography scan. Carotid intima‐media thickness (cIMT), Framingham risk score, atherosclerotic cardiovascular disease (ASCVD) score, and risk score developed by data collection on adverse effects of anti‐HIV drugs (D:A:D) were also measured. Increased IFN‐α, IFN‐β, and ISG56 levels were observed in all HIV‐1‐infected males compared to healthy controls (p < .001 for all genes analyzed). HIV‐1‐infected patients with a stenosis degree ≥50% showed a higher Framingham risk score (p = .019), which was correlated with IFN‐β and ISG56 levels. HIV‐1‐infected males with enhanced IFN‐I levels and stenosis displayed a higher ASCVD calculated risk (p = .011) and D:A:D score (p = .004). Also, there was a trend toward higher IFN‐α and ISG56 mRNA levels in HIV‐1‐positive patients with an increased cIMT (p > .05). Dysregulation of IFN‐I response might participate in the pathogenesis of HIV‐1‐associated CVD.
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spelling pubmed-83600152021-08-17 Alteration of type I interferon response is associated with subclinical atherosclerosis in virologically suppressed HIV‐1‐infected male patients Santinelli, Letizia De Girolamo, Gabriella Borrazzo, Cristian Vassalini, Paolo Pinacchio, Claudia Cavallari, Eugenio Nelson Statzu, Maura Frasca, Federica Scordio, Mirko Bitossi, Camilla Viscido, Agnese Ceccarelli, Giancarlo Mancone, Massimo Mastroianni, Claudio Maria Antonelli, Guido d'Ettorre, Gabriella Scagnolari, Carolina J Med Virol Research Articles Given human immunodeficiency virus‐1 (HIV‐1)‐infected patients have alterations in the type I interferon (IFN‐I) pathway and are also at elevated risk of atherosclerosis, we evaluated IFN‐I response and subclinical cardiovascular disease (CVD) association in HIV‐1‐infected patients. Transcript levels of IFN‐α/β and IFN‐stimulated gene 56 (ISG56) were evaluated by RT/real‐time PCR in peripheral blood mononuclear cells collected from asymptomatic HIV‐1‐positive male patients at high risk of developing CVD (n = 34) and healthy subjects (n = 21). Stenosis degree (≥ or <50%), calcium volume score, calcium Agatston score, and myocardial extracellular volume were examined by coronary computerized tomography scan. Carotid intima‐media thickness (cIMT), Framingham risk score, atherosclerotic cardiovascular disease (ASCVD) score, and risk score developed by data collection on adverse effects of anti‐HIV drugs (D:A:D) were also measured. Increased IFN‐α, IFN‐β, and ISG56 levels were observed in all HIV‐1‐infected males compared to healthy controls (p < .001 for all genes analyzed). HIV‐1‐infected patients with a stenosis degree ≥50% showed a higher Framingham risk score (p = .019), which was correlated with IFN‐β and ISG56 levels. HIV‐1‐infected males with enhanced IFN‐I levels and stenosis displayed a higher ASCVD calculated risk (p = .011) and D:A:D score (p = .004). Also, there was a trend toward higher IFN‐α and ISG56 mRNA levels in HIV‐1‐positive patients with an increased cIMT (p > .05). Dysregulation of IFN‐I response might participate in the pathogenesis of HIV‐1‐associated CVD. John Wiley and Sons Inc. 2021-05-03 2021-08 /pmc/articles/PMC8360015/ /pubmed/33913525 http://dx.doi.org/10.1002/jmv.27028 Text en © 2021 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Santinelli, Letizia
De Girolamo, Gabriella
Borrazzo, Cristian
Vassalini, Paolo
Pinacchio, Claudia
Cavallari, Eugenio Nelson
Statzu, Maura
Frasca, Federica
Scordio, Mirko
Bitossi, Camilla
Viscido, Agnese
Ceccarelli, Giancarlo
Mancone, Massimo
Mastroianni, Claudio Maria
Antonelli, Guido
d'Ettorre, Gabriella
Scagnolari, Carolina
Alteration of type I interferon response is associated with subclinical atherosclerosis in virologically suppressed HIV‐1‐infected male patients
title Alteration of type I interferon response is associated with subclinical atherosclerosis in virologically suppressed HIV‐1‐infected male patients
title_full Alteration of type I interferon response is associated with subclinical atherosclerosis in virologically suppressed HIV‐1‐infected male patients
title_fullStr Alteration of type I interferon response is associated with subclinical atherosclerosis in virologically suppressed HIV‐1‐infected male patients
title_full_unstemmed Alteration of type I interferon response is associated with subclinical atherosclerosis in virologically suppressed HIV‐1‐infected male patients
title_short Alteration of type I interferon response is associated with subclinical atherosclerosis in virologically suppressed HIV‐1‐infected male patients
title_sort alteration of type i interferon response is associated with subclinical atherosclerosis in virologically suppressed hiv‐1‐infected male patients
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360015/
https://www.ncbi.nlm.nih.gov/pubmed/33913525
http://dx.doi.org/10.1002/jmv.27028
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