Intra‐individual differences in the effect of endurance versus resistance training on vascular function: A cross‐over study

We used a within‐subject, cross‐over design study to compare the impact of 4‐weeks' resistance (RT) versus endurance (END) training on vascular function. We subsequently explored the association of intra‐individual effects of RT versus END on vascular function with a single nucleotide polymorphism (SNP) of the NOS3 gene. Thirty‐five healthy males (21 ± 2 years old) were genotyped for the NOS3 rs2070744 SNP and completed both training modalities. Participants completed 12 sessions over a 4‐week period, either RT (leg‐extension) or END (cycling) training in a randomized, balanced cross‐over design with a 3‐week washout period. Participants performed peak oxygen uptake (peak VO(2)) and leg‐extension single‐repetition maximum (1‐RM) testing, and vascular function assessment using flow‐mediated dilation (FMD) on 3 separated days pre/post‐training. Peak VO(2) increased after END (p < 0.001), while 1‐RM increased after RT (p < 0.001). FMD improved after 4‐weeks’ training (time effect: p = 0.006), with no difference between exercise modalities (interaction effect: p = 0.92). No relation was found between individual changes (delta, pre‐post) in FMD to both types of training (R(2) = 0.06, p = 0.14). Intra‐individual changes in FMD following END and RT were associated with the NOS3 SNP, with TT homozygotes significantly favoring only END (p = 0.016) and TC/CC tending to favor RT only (p = 0.056). Although both training modes improved vascular function, significant intra‐individual variation in the adaptation of FMD was found. The association with NOS3 genotype suggests a genetic predisposition to FMD adapting to a specific mode of chronic exercise. This study therefore provides novel evidence for personalized exercise training to optimize vascular health.