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SLI1 confers broad‐spectrum resistance to phloem‐feeding insects
Resistance (R) genes usually compete in a coevolutionary arms race with reciprocal effectors to confer strain‐specific resistance to pathogens or herbivorous insects. Here, we investigate the specificity of SLI1, a recently identified R gene in Arabidopsis that encodes a small heat shock‐like protei...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Ltd.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360143/ https://www.ncbi.nlm.nih.gov/pubmed/33837973 http://dx.doi.org/10.1111/pce.14064 |
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author | Kloth, Karen J. Shah, Parth Broekgaarden, Colette Ström, Cecilia Albrectsen, Benedicte R. Dicke, Marcel |
author_facet | Kloth, Karen J. Shah, Parth Broekgaarden, Colette Ström, Cecilia Albrectsen, Benedicte R. Dicke, Marcel |
author_sort | Kloth, Karen J. |
collection | PubMed |
description | Resistance (R) genes usually compete in a coevolutionary arms race with reciprocal effectors to confer strain‐specific resistance to pathogens or herbivorous insects. Here, we investigate the specificity of SLI1, a recently identified R gene in Arabidopsis that encodes a small heat shock‐like protein involved in resistance to Myzus persicae aphids. In a panel with several aphid and whitefly species, SLI1 compromised reproductive rates of three species: the tobacco aphid M. persicae nicotianae, the cabbage aphid Brevicoryne brassicae and the cabbage whitefly Aleyrodes proletella. Electrical penetration graph recording of aphid behaviour, revealed shorter salivations and a 3‐to‐5‐fold increase in phloem feeding on sli1 loss‐of‐function plants. The mustard aphid Lipaphis erysimi and Bemisia tabaci whitefly were not affected by SLI1. Unlike the other two aphid species, L. erysimi exhibited repetitive salivations preceding successful phloem feeding, indicating a role of salivary effectors in overcoming SLI1‐mediated resistance. Microscopic characterization showed that SLI1 proteins localize in the sieve tubes of virtually all above‐ and below‐ground tissues and co‐localize with the aphid stylet tip after penetration of the sieve element plasma membrane. These observations reveal an unconventional R gene that escapes the paradigm of strain specificity and confers broad‐spectrum quantitative resistance to phloem‐feeding insects. |
format | Online Article Text |
id | pubmed-8360143 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley & Sons, Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83601432021-08-17 SLI1 confers broad‐spectrum resistance to phloem‐feeding insects Kloth, Karen J. Shah, Parth Broekgaarden, Colette Ström, Cecilia Albrectsen, Benedicte R. Dicke, Marcel Plant Cell Environ Original Articles Resistance (R) genes usually compete in a coevolutionary arms race with reciprocal effectors to confer strain‐specific resistance to pathogens or herbivorous insects. Here, we investigate the specificity of SLI1, a recently identified R gene in Arabidopsis that encodes a small heat shock‐like protein involved in resistance to Myzus persicae aphids. In a panel with several aphid and whitefly species, SLI1 compromised reproductive rates of three species: the tobacco aphid M. persicae nicotianae, the cabbage aphid Brevicoryne brassicae and the cabbage whitefly Aleyrodes proletella. Electrical penetration graph recording of aphid behaviour, revealed shorter salivations and a 3‐to‐5‐fold increase in phloem feeding on sli1 loss‐of‐function plants. The mustard aphid Lipaphis erysimi and Bemisia tabaci whitefly were not affected by SLI1. Unlike the other two aphid species, L. erysimi exhibited repetitive salivations preceding successful phloem feeding, indicating a role of salivary effectors in overcoming SLI1‐mediated resistance. Microscopic characterization showed that SLI1 proteins localize in the sieve tubes of virtually all above‐ and below‐ground tissues and co‐localize with the aphid stylet tip after penetration of the sieve element plasma membrane. These observations reveal an unconventional R gene that escapes the paradigm of strain specificity and confers broad‐spectrum quantitative resistance to phloem‐feeding insects. John Wiley & Sons, Ltd. 2021-05-05 2021-08 /pmc/articles/PMC8360143/ /pubmed/33837973 http://dx.doi.org/10.1111/pce.14064 Text en © 2021 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Kloth, Karen J. Shah, Parth Broekgaarden, Colette Ström, Cecilia Albrectsen, Benedicte R. Dicke, Marcel SLI1 confers broad‐spectrum resistance to phloem‐feeding insects |
title | SLI1 confers broad‐spectrum resistance to phloem‐feeding insects |
title_full | SLI1 confers broad‐spectrum resistance to phloem‐feeding insects |
title_fullStr | SLI1 confers broad‐spectrum resistance to phloem‐feeding insects |
title_full_unstemmed | SLI1 confers broad‐spectrum resistance to phloem‐feeding insects |
title_short | SLI1 confers broad‐spectrum resistance to phloem‐feeding insects |
title_sort | sli1 confers broad‐spectrum resistance to phloem‐feeding insects |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360143/ https://www.ncbi.nlm.nih.gov/pubmed/33837973 http://dx.doi.org/10.1111/pce.14064 |
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