Cargando…
The chemical compound ‘Heatin’ stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1‐subfamily of nitrilases
Temperature passively affects biological processes involved in plant growth. Therefore, it is challenging to study the dedicated temperature signalling pathways that orchestrate thermomorphogenesis, a suite of elongation growth‐based adaptations that enhance leaf‐cooling capacity. We screened a chem...
Autores principales: | , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360157/ https://www.ncbi.nlm.nih.gov/pubmed/33768644 http://dx.doi.org/10.1111/tpj.15250 |
_version_ | 1783737689699254272 |
---|---|
author | van der Woude, Lennard Piotrowski, Markus Klaasse, Gruson Paulus, Judith K. Krahn, Daniel Ninck, Sabrina Kaschani, Farnusch Kaiser, Markus Novák, Ondřej Ljung, Karin Bulder, Suzanne van Verk, Marcel Snoek, Basten L. Fiers, Martijn Martin, Nathaniel I. van der Hoorn, Renier A. L. Robert, Stéphanie Smeekens, Sjef van Zanten, Martijn |
author_facet | van der Woude, Lennard Piotrowski, Markus Klaasse, Gruson Paulus, Judith K. Krahn, Daniel Ninck, Sabrina Kaschani, Farnusch Kaiser, Markus Novák, Ondřej Ljung, Karin Bulder, Suzanne van Verk, Marcel Snoek, Basten L. Fiers, Martijn Martin, Nathaniel I. van der Hoorn, Renier A. L. Robert, Stéphanie Smeekens, Sjef van Zanten, Martijn |
author_sort | van der Woude, Lennard |
collection | PubMed |
description | Temperature passively affects biological processes involved in plant growth. Therefore, it is challenging to study the dedicated temperature signalling pathways that orchestrate thermomorphogenesis, a suite of elongation growth‐based adaptations that enhance leaf‐cooling capacity. We screened a chemical library for compounds that restored hypocotyl elongation in the pif4‐2–deficient mutant background at warm temperature conditions in Arabidopsis thaliana to identify modulators of thermomorphogenesis. The small aromatic compound ‘Heatin’, containing 1‐iminomethyl‐2‐naphthol as a pharmacophore, was selected as an enhancer of elongation growth. We show that ARABIDOPSIS ALDEHYDE OXIDASES redundantly contribute to Heatin‐mediated hypocotyl elongation. Following a chemical proteomics approach, the members of the NITRILASE1‐subfamily of auxin biosynthesis enzymes were identified among the molecular targets of Heatin. Our data reveal that nitrilases are involved in promotion of hypocotyl elongation in response to high temperature and Heatin‐mediated hypocotyl elongation requires the NITRILASE1‐subfamily members, NIT1 and NIT2. Heatin inhibits NIT1‐subfamily enzymatic activity in vitro and the application of Heatin accordingly results in the accumulation of NIT1‐subfamily substrate indole‐3‐acetonitrile in vivo. However, levels of the NIT1‐subfamily product, bioactive auxin (indole‐3‐acetic acid), were also significantly increased. It is likely that the stimulation of hypocotyl elongation by Heatin might be independent of its observed interaction with NITRILASE1‐subfamily members. However, nitrilases may contribute to the Heatin response by stimulating indole‐3‐acetic acid biosynthesis in an indirect way. Heatin and its functional analogues present novel chemical entities for studying auxin biology. |
format | Online Article Text |
id | pubmed-8360157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83601572021-08-17 The chemical compound ‘Heatin’ stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1‐subfamily of nitrilases van der Woude, Lennard Piotrowski, Markus Klaasse, Gruson Paulus, Judith K. Krahn, Daniel Ninck, Sabrina Kaschani, Farnusch Kaiser, Markus Novák, Ondřej Ljung, Karin Bulder, Suzanne van Verk, Marcel Snoek, Basten L. Fiers, Martijn Martin, Nathaniel I. van der Hoorn, Renier A. L. Robert, Stéphanie Smeekens, Sjef van Zanten, Martijn Plant J Original Articles Temperature passively affects biological processes involved in plant growth. Therefore, it is challenging to study the dedicated temperature signalling pathways that orchestrate thermomorphogenesis, a suite of elongation growth‐based adaptations that enhance leaf‐cooling capacity. We screened a chemical library for compounds that restored hypocotyl elongation in the pif4‐2–deficient mutant background at warm temperature conditions in Arabidopsis thaliana to identify modulators of thermomorphogenesis. The small aromatic compound ‘Heatin’, containing 1‐iminomethyl‐2‐naphthol as a pharmacophore, was selected as an enhancer of elongation growth. We show that ARABIDOPSIS ALDEHYDE OXIDASES redundantly contribute to Heatin‐mediated hypocotyl elongation. Following a chemical proteomics approach, the members of the NITRILASE1‐subfamily of auxin biosynthesis enzymes were identified among the molecular targets of Heatin. Our data reveal that nitrilases are involved in promotion of hypocotyl elongation in response to high temperature and Heatin‐mediated hypocotyl elongation requires the NITRILASE1‐subfamily members, NIT1 and NIT2. Heatin inhibits NIT1‐subfamily enzymatic activity in vitro and the application of Heatin accordingly results in the accumulation of NIT1‐subfamily substrate indole‐3‐acetonitrile in vivo. However, levels of the NIT1‐subfamily product, bioactive auxin (indole‐3‐acetic acid), were also significantly increased. It is likely that the stimulation of hypocotyl elongation by Heatin might be independent of its observed interaction with NITRILASE1‐subfamily members. However, nitrilases may contribute to the Heatin response by stimulating indole‐3‐acetic acid biosynthesis in an indirect way. Heatin and its functional analogues present novel chemical entities for studying auxin biology. John Wiley and Sons Inc. 2021-05-06 2021-06 /pmc/articles/PMC8360157/ /pubmed/33768644 http://dx.doi.org/10.1111/tpj.15250 Text en © 2021 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles van der Woude, Lennard Piotrowski, Markus Klaasse, Gruson Paulus, Judith K. Krahn, Daniel Ninck, Sabrina Kaschani, Farnusch Kaiser, Markus Novák, Ondřej Ljung, Karin Bulder, Suzanne van Verk, Marcel Snoek, Basten L. Fiers, Martijn Martin, Nathaniel I. van der Hoorn, Renier A. L. Robert, Stéphanie Smeekens, Sjef van Zanten, Martijn The chemical compound ‘Heatin’ stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1‐subfamily of nitrilases |
title | The chemical compound ‘Heatin’ stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1‐subfamily of nitrilases |
title_full | The chemical compound ‘Heatin’ stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1‐subfamily of nitrilases |
title_fullStr | The chemical compound ‘Heatin’ stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1‐subfamily of nitrilases |
title_full_unstemmed | The chemical compound ‘Heatin’ stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1‐subfamily of nitrilases |
title_short | The chemical compound ‘Heatin’ stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1‐subfamily of nitrilases |
title_sort | chemical compound ‘heatin’ stimulates hypocotyl elongation and interferes with the arabidopsis nit1‐subfamily of nitrilases |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360157/ https://www.ncbi.nlm.nih.gov/pubmed/33768644 http://dx.doi.org/10.1111/tpj.15250 |
work_keys_str_mv | AT vanderwoudelennard thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT piotrowskimarkus thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT klaassegruson thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT paulusjudithk thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT krahndaniel thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT nincksabrina thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT kaschanifarnusch thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT kaisermarkus thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT novakondrej thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT ljungkarin thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT buldersuzanne thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT vanverkmarcel thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT snoekbastenl thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT fiersmartijn thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT martinnathanieli thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT vanderhoornrenieral thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT robertstephanie thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT smeekenssjef thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT vanzantenmartijn thechemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT vanderwoudelennard chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT piotrowskimarkus chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT klaassegruson chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT paulusjudithk chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT krahndaniel chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT nincksabrina chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT kaschanifarnusch chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT kaisermarkus chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT novakondrej chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT ljungkarin chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT buldersuzanne chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT vanverkmarcel chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT snoekbastenl chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT fiersmartijn chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT martinnathanieli chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT vanderhoornrenieral chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT robertstephanie chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT smeekenssjef chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases AT vanzantenmartijn chemicalcompoundheatinstimulateshypocotylelongationandinterfereswiththearabidopsisnit1subfamilyofnitrilases |