Cargando…
Differential Proteomic Analysis of the Hippocampus in Rats with Neuropathic Pain to Investigate the Use of Electroacupuncture in Relieving Mechanical Allodynia and Cognitive Decline
Abnormal changes in hippocampal function and neuroplasticity are involved in neuropathic pain, which induces hyperalgesia and learning and memory deficits. Previous studies from our group have shown that electroacupuncture at Huantiao (GB30) and Yanglingquan (GB34) has an obvious analgesic effect on...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360723/ https://www.ncbi.nlm.nih.gov/pubmed/34394341 http://dx.doi.org/10.1155/2021/5597163 |
_version_ | 1783737800873476096 |
---|---|
author | Gong, Degui Yu, Xiangmei Jiang, Menghong Li, Changzheng Wang, Zhifu |
author_facet | Gong, Degui Yu, Xiangmei Jiang, Menghong Li, Changzheng Wang, Zhifu |
author_sort | Gong, Degui |
collection | PubMed |
description | Abnormal changes in hippocampal function and neuroplasticity are involved in neuropathic pain, which induces hyperalgesia and learning and memory deficits. Previous studies from our group have shown that electroacupuncture at Huantiao (GB30) and Yanglingquan (GB34) has an obvious analgesic effect on neuropathic pain. However, the central regulatory mechanism occurring in the hippocampus remains to be investigated. In this study, behavioral and proteomic analyses were performed to identify differentially expressed hippocampal proteins involved in electroacupuncture-induced analgesia. Our results showed both upregulated (TMEM126A, RDH13, and Luc7L) and downregulated proteins (Mettl7A, GGA1 RTKN, RSBN1, and CDKN1B). Further protein verification revealed for the first time that hippocampal TMEM126A plays an important anti-inflammatory role in the treatment of neuralgia by electroacupuncture. |
format | Online Article Text |
id | pubmed-8360723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-83607232021-08-13 Differential Proteomic Analysis of the Hippocampus in Rats with Neuropathic Pain to Investigate the Use of Electroacupuncture in Relieving Mechanical Allodynia and Cognitive Decline Gong, Degui Yu, Xiangmei Jiang, Menghong Li, Changzheng Wang, Zhifu Neural Plast Research Article Abnormal changes in hippocampal function and neuroplasticity are involved in neuropathic pain, which induces hyperalgesia and learning and memory deficits. Previous studies from our group have shown that electroacupuncture at Huantiao (GB30) and Yanglingquan (GB34) has an obvious analgesic effect on neuropathic pain. However, the central regulatory mechanism occurring in the hippocampus remains to be investigated. In this study, behavioral and proteomic analyses were performed to identify differentially expressed hippocampal proteins involved in electroacupuncture-induced analgesia. Our results showed both upregulated (TMEM126A, RDH13, and Luc7L) and downregulated proteins (Mettl7A, GGA1 RTKN, RSBN1, and CDKN1B). Further protein verification revealed for the first time that hippocampal TMEM126A plays an important anti-inflammatory role in the treatment of neuralgia by electroacupuncture. Hindawi 2021-08-04 /pmc/articles/PMC8360723/ /pubmed/34394341 http://dx.doi.org/10.1155/2021/5597163 Text en Copyright © 2021 Degui Gong et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gong, Degui Yu, Xiangmei Jiang, Menghong Li, Changzheng Wang, Zhifu Differential Proteomic Analysis of the Hippocampus in Rats with Neuropathic Pain to Investigate the Use of Electroacupuncture in Relieving Mechanical Allodynia and Cognitive Decline |
title | Differential Proteomic Analysis of the Hippocampus in Rats with Neuropathic Pain to Investigate the Use of Electroacupuncture in Relieving Mechanical Allodynia and Cognitive Decline |
title_full | Differential Proteomic Analysis of the Hippocampus in Rats with Neuropathic Pain to Investigate the Use of Electroacupuncture in Relieving Mechanical Allodynia and Cognitive Decline |
title_fullStr | Differential Proteomic Analysis of the Hippocampus in Rats with Neuropathic Pain to Investigate the Use of Electroacupuncture in Relieving Mechanical Allodynia and Cognitive Decline |
title_full_unstemmed | Differential Proteomic Analysis of the Hippocampus in Rats with Neuropathic Pain to Investigate the Use of Electroacupuncture in Relieving Mechanical Allodynia and Cognitive Decline |
title_short | Differential Proteomic Analysis of the Hippocampus in Rats with Neuropathic Pain to Investigate the Use of Electroacupuncture in Relieving Mechanical Allodynia and Cognitive Decline |
title_sort | differential proteomic analysis of the hippocampus in rats with neuropathic pain to investigate the use of electroacupuncture in relieving mechanical allodynia and cognitive decline |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360723/ https://www.ncbi.nlm.nih.gov/pubmed/34394341 http://dx.doi.org/10.1155/2021/5597163 |
work_keys_str_mv | AT gongdegui differentialproteomicanalysisofthehippocampusinratswithneuropathicpaintoinvestigatetheuseofelectroacupunctureinrelievingmechanicalallodyniaandcognitivedecline AT yuxiangmei differentialproteomicanalysisofthehippocampusinratswithneuropathicpaintoinvestigatetheuseofelectroacupunctureinrelievingmechanicalallodyniaandcognitivedecline AT jiangmenghong differentialproteomicanalysisofthehippocampusinratswithneuropathicpaintoinvestigatetheuseofelectroacupunctureinrelievingmechanicalallodyniaandcognitivedecline AT lichangzheng differentialproteomicanalysisofthehippocampusinratswithneuropathicpaintoinvestigatetheuseofelectroacupunctureinrelievingmechanicalallodyniaandcognitivedecline AT wangzhifu differentialproteomicanalysisofthehippocampusinratswithneuropathicpaintoinvestigatetheuseofelectroacupunctureinrelievingmechanicalallodyniaandcognitivedecline |