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Chemical Composition and Antimicrobial Activity of Subcritical CO(2) Extract of Lepidium latifolium L. (Brassicaceae)

The genus Lepidium L. from Brassicaceae Burnett. family covers over 150 species with an almost cosmopolitan spread. In Kazakhstan, 21 species are described, of which four species are characterized by medicinal properties (L. crassifolium Waldst. et Kit., L. perfoliatum L., L. ruderale L., and L. lat...

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Autores principales: Azimkhanova, Balzhan B., Ustenova, Gulbaram O., Sharipov, Kamalidin O., Rakhimov, Kayrolla D., Sayakova, Galiya M., Jumagaziyeva, Ardak B., Flisyuk, Elena V., Gemejiyeva, Nadezhda G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360737/
https://www.ncbi.nlm.nih.gov/pubmed/34394355
http://dx.doi.org/10.1155/2021/4389967
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author Azimkhanova, Balzhan B.
Ustenova, Gulbaram O.
Sharipov, Kamalidin O.
Rakhimov, Kayrolla D.
Sayakova, Galiya M.
Jumagaziyeva, Ardak B.
Flisyuk, Elena V.
Gemejiyeva, Nadezhda G.
author_facet Azimkhanova, Balzhan B.
Ustenova, Gulbaram O.
Sharipov, Kamalidin O.
Rakhimov, Kayrolla D.
Sayakova, Galiya M.
Jumagaziyeva, Ardak B.
Flisyuk, Elena V.
Gemejiyeva, Nadezhda G.
author_sort Azimkhanova, Balzhan B.
collection PubMed
description The genus Lepidium L. from Brassicaceae Burnett. family covers over 150 species with an almost cosmopolitan spread. In Kazakhstan, 21 species are described, of which four species are characterized by medicinal properties (L. crassifolium Waldst. et Kit., L. perfoliatum L., L. ruderale L., and L. latifolium L.), used in folk medicine as means of antibacterial, irritant, laxative, antitumor, analgesic, and anthelmintic action. Methods. Raw materials were collected from Almaty region (Republic of Kazakhstan). Lepidium latifolium L. herb's carbon dioxide extract (CO(2) extract) was obtained by subcritical carbon dioxide extraction. A gas chromatograph with a mass spectrometric detector was used to determine the component composition of the extract. Antimicrobial activity was determined by two methods: the micromethod of serial dilution and the disc-diffusion method. Four microbial test strains were used: Staphylococcus aureus ATCC 6538-P, Escherichia coli ATCC 8739, Klebsiella pneumonia ATCC 10031, and Candida albicans ATCC 10231. Results. The technology of carbon dioxide extraction has undoubted advantages over traditional methods of extraction: it has a controlled selectivity in relation to groups of biologically active substances, allows deep extraction, and maximizes the release of rich complexes of compounds contained in plants. In this study, firstly, the CO(2) extract was obtained under subcritical conditions from the aerial part of L. latifolium L., and the composition was determined. Hexane was the best solvent for CO(2) extract, and 40 components were identified. Screening of antimicrobial activity of the L. latifolium's CO(2) extract showed the essential activity of all clinically significant strains tested: Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, and Candida albicans. Conclusions. This research showed that the CO(2) extract of the raw material of Lepidium latifolium L. contains biologically active compounds exhibiting an essential antimicrobial effect, and therefore it is possible to recommend for the development of various drugs for use in medical practice.
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spelling pubmed-83607372021-08-13 Chemical Composition and Antimicrobial Activity of Subcritical CO(2) Extract of Lepidium latifolium L. (Brassicaceae) Azimkhanova, Balzhan B. Ustenova, Gulbaram O. Sharipov, Kamalidin O. Rakhimov, Kayrolla D. Sayakova, Galiya M. Jumagaziyeva, Ardak B. Flisyuk, Elena V. Gemejiyeva, Nadezhda G. Int J Biomater Research Article The genus Lepidium L. from Brassicaceae Burnett. family covers over 150 species with an almost cosmopolitan spread. In Kazakhstan, 21 species are described, of which four species are characterized by medicinal properties (L. crassifolium Waldst. et Kit., L. perfoliatum L., L. ruderale L., and L. latifolium L.), used in folk medicine as means of antibacterial, irritant, laxative, antitumor, analgesic, and anthelmintic action. Methods. Raw materials were collected from Almaty region (Republic of Kazakhstan). Lepidium latifolium L. herb's carbon dioxide extract (CO(2) extract) was obtained by subcritical carbon dioxide extraction. A gas chromatograph with a mass spectrometric detector was used to determine the component composition of the extract. Antimicrobial activity was determined by two methods: the micromethod of serial dilution and the disc-diffusion method. Four microbial test strains were used: Staphylococcus aureus ATCC 6538-P, Escherichia coli ATCC 8739, Klebsiella pneumonia ATCC 10031, and Candida albicans ATCC 10231. Results. The technology of carbon dioxide extraction has undoubted advantages over traditional methods of extraction: it has a controlled selectivity in relation to groups of biologically active substances, allows deep extraction, and maximizes the release of rich complexes of compounds contained in plants. In this study, firstly, the CO(2) extract was obtained under subcritical conditions from the aerial part of L. latifolium L., and the composition was determined. Hexane was the best solvent for CO(2) extract, and 40 components were identified. Screening of antimicrobial activity of the L. latifolium's CO(2) extract showed the essential activity of all clinically significant strains tested: Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, and Candida albicans. Conclusions. This research showed that the CO(2) extract of the raw material of Lepidium latifolium L. contains biologically active compounds exhibiting an essential antimicrobial effect, and therefore it is possible to recommend for the development of various drugs for use in medical practice. Hindawi 2021-08-04 /pmc/articles/PMC8360737/ /pubmed/34394355 http://dx.doi.org/10.1155/2021/4389967 Text en Copyright © 2021 Balzhan B. Azimkhanova et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Azimkhanova, Balzhan B.
Ustenova, Gulbaram O.
Sharipov, Kamalidin O.
Rakhimov, Kayrolla D.
Sayakova, Galiya M.
Jumagaziyeva, Ardak B.
Flisyuk, Elena V.
Gemejiyeva, Nadezhda G.
Chemical Composition and Antimicrobial Activity of Subcritical CO(2) Extract of Lepidium latifolium L. (Brassicaceae)
title Chemical Composition and Antimicrobial Activity of Subcritical CO(2) Extract of Lepidium latifolium L. (Brassicaceae)
title_full Chemical Composition and Antimicrobial Activity of Subcritical CO(2) Extract of Lepidium latifolium L. (Brassicaceae)
title_fullStr Chemical Composition and Antimicrobial Activity of Subcritical CO(2) Extract of Lepidium latifolium L. (Brassicaceae)
title_full_unstemmed Chemical Composition and Antimicrobial Activity of Subcritical CO(2) Extract of Lepidium latifolium L. (Brassicaceae)
title_short Chemical Composition and Antimicrobial Activity of Subcritical CO(2) Extract of Lepidium latifolium L. (Brassicaceae)
title_sort chemical composition and antimicrobial activity of subcritical co(2) extract of lepidium latifolium l. (brassicaceae)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360737/
https://www.ncbi.nlm.nih.gov/pubmed/34394355
http://dx.doi.org/10.1155/2021/4389967
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