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Natural microbial polysaccharides as effective factors for modification of the catalytic properties of fungal cellobiose dehydrogenase
Polysaccharides are biopolymers composed of simple sugars like glucose, galactose, mannose, fructose, etc. The major natural sources for the production of polysaccharides include plants and microorganisms. In the present work, four bacterial and two fungal polysaccharides (PS or EPS) were used for t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360876/ https://www.ncbi.nlm.nih.gov/pubmed/34132850 http://dx.doi.org/10.1007/s00203-021-02424-1 |
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author | Sulej, Justyna Jaszek, Magdalena Osińska-Jaroszuk, Monika Matuszewska, Anna Bancerz, Renata Janczarek, Monika |
author_facet | Sulej, Justyna Jaszek, Magdalena Osińska-Jaroszuk, Monika Matuszewska, Anna Bancerz, Renata Janczarek, Monika |
author_sort | Sulej, Justyna |
collection | PubMed |
description | Polysaccharides are biopolymers composed of simple sugars like glucose, galactose, mannose, fructose, etc. The major natural sources for the production of polysaccharides include plants and microorganisms. In the present work, four bacterial and two fungal polysaccharides (PS or EPS) were used for the modification and preservation of Pycnoporus sanguineus cellobiose dehydrogenase (CDH) activity. It was found that the presence of polysaccharide preparations clearly enhanced the stability of cellobiose dehydrogenase compared to the control value (4 °C). The highest stabilization effect was observed for CDH modified with Rh110EPS. Changes in the optimum pH in the samples of CDH incubated with the chosen polysaccharide modifiers were evidenced as well. The most significant effect was observed for Rh24EPS and Cu139PS (pH 3.5). Cyclic voltammetry used for the analysis of electrochemical parameters of modified CDH showed the highest peak values after 30 days of incubation with polysaccharides at 4 °C. In summary, natural polysaccharides seem to be an effective biotechnological tool for the modification of CDH activity to increase the possibilities of its practical applications in many fields of industry. |
format | Online Article Text |
id | pubmed-8360876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-83608762021-08-30 Natural microbial polysaccharides as effective factors for modification of the catalytic properties of fungal cellobiose dehydrogenase Sulej, Justyna Jaszek, Magdalena Osińska-Jaroszuk, Monika Matuszewska, Anna Bancerz, Renata Janczarek, Monika Arch Microbiol Original Paper Polysaccharides are biopolymers composed of simple sugars like glucose, galactose, mannose, fructose, etc. The major natural sources for the production of polysaccharides include plants and microorganisms. In the present work, four bacterial and two fungal polysaccharides (PS or EPS) were used for the modification and preservation of Pycnoporus sanguineus cellobiose dehydrogenase (CDH) activity. It was found that the presence of polysaccharide preparations clearly enhanced the stability of cellobiose dehydrogenase compared to the control value (4 °C). The highest stabilization effect was observed for CDH modified with Rh110EPS. Changes in the optimum pH in the samples of CDH incubated with the chosen polysaccharide modifiers were evidenced as well. The most significant effect was observed for Rh24EPS and Cu139PS (pH 3.5). Cyclic voltammetry used for the analysis of electrochemical parameters of modified CDH showed the highest peak values after 30 days of incubation with polysaccharides at 4 °C. In summary, natural polysaccharides seem to be an effective biotechnological tool for the modification of CDH activity to increase the possibilities of its practical applications in many fields of industry. Springer Berlin Heidelberg 2021-06-16 2021 /pmc/articles/PMC8360876/ /pubmed/34132850 http://dx.doi.org/10.1007/s00203-021-02424-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Paper Sulej, Justyna Jaszek, Magdalena Osińska-Jaroszuk, Monika Matuszewska, Anna Bancerz, Renata Janczarek, Monika Natural microbial polysaccharides as effective factors for modification of the catalytic properties of fungal cellobiose dehydrogenase |
title | Natural microbial polysaccharides as effective factors for modification of the catalytic properties of fungal cellobiose dehydrogenase |
title_full | Natural microbial polysaccharides as effective factors for modification of the catalytic properties of fungal cellobiose dehydrogenase |
title_fullStr | Natural microbial polysaccharides as effective factors for modification of the catalytic properties of fungal cellobiose dehydrogenase |
title_full_unstemmed | Natural microbial polysaccharides as effective factors for modification of the catalytic properties of fungal cellobiose dehydrogenase |
title_short | Natural microbial polysaccharides as effective factors for modification of the catalytic properties of fungal cellobiose dehydrogenase |
title_sort | natural microbial polysaccharides as effective factors for modification of the catalytic properties of fungal cellobiose dehydrogenase |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360876/ https://www.ncbi.nlm.nih.gov/pubmed/34132850 http://dx.doi.org/10.1007/s00203-021-02424-1 |
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