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Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force
Techniques used to clear biological tissue for fluorescence microscopy are essential to connect anatomical principles at levels ranging from subcellular to the whole animal. Here we report a simple and straightforward approach to efficiently render opaque tissue samples transparent and show that thi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360944/ https://www.ncbi.nlm.nih.gov/pubmed/34385489 http://dx.doi.org/10.1038/s41598-021-95692-2 |
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author | Dwyer, Joseph Ramirez, M. Desmond Katz, Paul S. Karlstrom, Rolf O. Bergan, Joseph |
author_facet | Dwyer, Joseph Ramirez, M. Desmond Katz, Paul S. Karlstrom, Rolf O. Bergan, Joseph |
author_sort | Dwyer, Joseph |
collection | PubMed |
description | Techniques used to clear biological tissue for fluorescence microscopy are essential to connect anatomical principles at levels ranging from subcellular to the whole animal. Here we report a simple and straightforward approach to efficiently render opaque tissue samples transparent and show that this approach can be modified to rapidly label intact tissue samples with antibodies for large volume fluorescence microscopy. This strategy applies a magnetohydrodynamic (MHD) force to accelerate the removal of lipids from tissue samples at least as large as an intact adult mouse brain. We also show that MHD force can be used to accelerate antibody penetration into tissue samples. This strategy complements a growing array of tools that enable high-resolution 3-dimensional anatomical analyses in intact tissues using fluorescence microscopy. MHD-accelerated clearing is simple, fast, reliable, inexpensive, provides good thermal regulation, and is compatible with existing strategies for high-quality fluorescence microscopy of intact tissues. |
format | Online Article Text |
id | pubmed-8360944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83609442021-08-13 Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force Dwyer, Joseph Ramirez, M. Desmond Katz, Paul S. Karlstrom, Rolf O. Bergan, Joseph Sci Rep Article Techniques used to clear biological tissue for fluorescence microscopy are essential to connect anatomical principles at levels ranging from subcellular to the whole animal. Here we report a simple and straightforward approach to efficiently render opaque tissue samples transparent and show that this approach can be modified to rapidly label intact tissue samples with antibodies for large volume fluorescence microscopy. This strategy applies a magnetohydrodynamic (MHD) force to accelerate the removal of lipids from tissue samples at least as large as an intact adult mouse brain. We also show that MHD force can be used to accelerate antibody penetration into tissue samples. This strategy complements a growing array of tools that enable high-resolution 3-dimensional anatomical analyses in intact tissues using fluorescence microscopy. MHD-accelerated clearing is simple, fast, reliable, inexpensive, provides good thermal regulation, and is compatible with existing strategies for high-quality fluorescence microscopy of intact tissues. Nature Publishing Group UK 2021-08-12 /pmc/articles/PMC8360944/ /pubmed/34385489 http://dx.doi.org/10.1038/s41598-021-95692-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Dwyer, Joseph Ramirez, M. Desmond Katz, Paul S. Karlstrom, Rolf O. Bergan, Joseph Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force |
title | Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force |
title_full | Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force |
title_fullStr | Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force |
title_full_unstemmed | Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force |
title_short | Accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force |
title_sort | accelerated clearing and molecular labeling of biological tissues using magnetohydrodynamic force |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360944/ https://www.ncbi.nlm.nih.gov/pubmed/34385489 http://dx.doi.org/10.1038/s41598-021-95692-2 |
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